Myotubularin Antibody

Images

 
Western Blot: Myotubularin Antibody [NB300-949] - Staining of Mouse Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Myotubularin Antibody [NB300-949] - Staining of paraffin embedded Human Uterus. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Myotubularin Antibody [NB300-949] - Staining (0.3ug/ml) of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Western Blot: Myotubularin Antibody [NB300-949] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Western Blot: Myotubularin Antibody [NB300-949] - Staining of Mouse Heart (A) and Skeletal Muscle (B) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Western Blot: Myotubularin Antibody [NB300-949] - HEK293 overexpressing MTM1 and probed with (mock transfection in first lane).

Product Details

Summary
Reactivity Hu, Mu, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Myotubularin Antibody Summary

Immunogen
Peptide with sequence C-SSPSQMMPHVQTHF corresponding to C-Terminus according to NP_000243.1.
Predicted Species
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MTM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 10 ug/mL
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/mL
Application Notes
WB: Approx. 70 kDa band observed in lysates of human skeletal muscle, mouse heart and skeletal muscle and HepG2 (calculated MW of 69.9 kDa band according to NP_000243.1and 69.6 kDa band according to mouse NP_001157662.1).
Control
Myotubularin Overexpression Lysate
Publications
Read Publication using NB300-949.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Myotubularin Antibody

  • CG2
  • CNM
  • EC 3.1.3.48
  • MTMX
  • myotubular myopathy 1
  • myotubularin 1
  • myotubularin
  • XLMTM

Background

This gene is a member of a gene family that encodes tyrosine phosphatases. Myotubularin is required for muscle cell differentiation and mutations in this gene have been identified as being responsible for X-linked myotubular myopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myotubularin Antibody (NB300-949)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MTM1
Entrez
Uniprot