Human ADAMTS13 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human ADAMTS13 Quantikine ELISA Kit Summary

• Background: The Quantikine Human ADAMTS13 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ADAMTS13 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human ADAMTS13 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ADAMTS13 showed linear curves that were parallel to the standard curves obt...ained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ADAMTS13.
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• Specificity: Natural and recombinant human ADAMTS13. This kit also recognizes a truncated version of recombinant human ADAMTS13 (amino acids Gln34-Trp688; Accession # NP_620594).
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ADAMTS13

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human ADAMTS13 Quantikine ELISA Kit

• A disintegrin and metalloproteinase with thrombospondin motifs 13
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13
• ADAM metallopeptidase with thrombospondin type 1 motif, 13
• ADAMTS13
• ADAM-TS13
• ADAMTS-13
• DKFZp434C2322
• EC 3.4.24.14
• EC 3.4.24.82
• EC 3.4.24.87
• FLJ42993
• MGC118899
• MGC118900
• TTP
• TTPADAM-TS 13
• vWF-cleaving protease
• vWF-CP
• vWF-CPC9orf8
• VWFCPvon Willebrand factor-cleaving protease

Background

Members of the ADAMTS family contain pro, catalytic, disintegrin-like, cysteine-rich, spacer and a variable number of TS type 1 motifs. In contrast to membrane-anchored ADAMs, ADAMTSs are secreted and are all active proteases. They are essential for aggrecan turnover and procollagen processing, regulation of which is important in many physiological and pathological processes such as angiogenesis and arthritis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for ADAMTS13 (DADT130)(11)

Publications using DADT130

• Zhang, Q;Bignotti, A;Yada, N;Ye, Z;Liu, S;Han, Z;Zheng, XL; Dynamic Assessment of Plasma von Willebrand Factor and ADAMTS13 Predicts Mortality in Hospitalized Patients with SARS-CoV-2 Infection Journal of clinical medicine 2023-11-19 [PMID: 38002786] (Human)
• Y Bi, J Su, S Zhou, Y Zhao, Y Zhang, H Zhang, M Liu, A Zhou, J Xu, M Pan, Y Zhao, F Li Distinct impact of IgG subclass on autoantibody pathogenicity in different IgG4-mediated diseases Elife, 2022-08-03;11(0):. 2022-08-03 [PMID: 35920621] (Human)
• K Singh, AC Kwong, H Madarati, S Kunasekara, T Sparring, AE Fox-Robich, PC Liaw, CA Kretz Characterization of ADAMTS13 and von Willebrand factor levels in septic and non-septic ICU patients PLoS ONE, 2021-02-19;16(2):e0247017. 2021-02-19 [PMID: 33606732] (Human)
• T Pascreau, S Zia-Chahab, B Zuber, C Tcherakian, E Farfour, M Vasse ADAMTS 13 deficiency is associated with abnormal distribution of von Willebrand factor multimers in patients with COVID-19 Thrombosis Research, 2021-02-09;0(0):. 2021-02-09 [PMID: 33583574] (Human)
• B Setiawan, CO Permatadew, B de Samakto, A Bugis, RM Naibaho, EA Pangarsa, D Santosa, C Suharti Von Willebrand factor:antigen and ADAMTS-13 level, but not soluble P-selectin, are risk factors for the first asymptomatic deep vein thrombosis in cancer patients undergoing chemotherapy Thrombosis journal, 2020-11-11;18(1):33. 2020-11-11 [PMID: 33292287] (Human)
• T Miku?a, J Koz?owska, W Sta?czak, M Sapu?a, A Ró?yk, A Wierci?ska Serum ADAMTS-13 Levels as an Indicator of Portal Vein Thrombosis Gastroenterol Res Pract, 2018-04-18;2018(0):3287491. 2018-04-18 [PMID: 29849584] (Human)
• S Liu-Chen, B Connolly, L Cheng, RR Subramania, Z Han mRNA treatment produces sustained expression of enzymatically active human ADAMTS13 in mice Sci Rep, 2018-05-18;8(1):7859. 2018-05-18 [PMID: 29777164] (Human, Mouse)
• NA Khalifa, HH Gawish, NA Khalifa, DM Tawfeek, SM Morsy ADAMTS-13 level in children with severe diarrhea-associated hemolytic uremic syndrome: Unmasking new association Saudi J Kidney Dis Transpl, 2018-03-01;29(2):303-309. 2018-03-01 [PMID: 29657197] (Human)
• Clinical and prognostic significance of serum levels of von Willebrand factor and ADAMTS-13 antigens in AL amyloidosis Blood, 2016-05-10;0(0):. 2016-05-10 [PMID: 27166361] (Human)
• Muroi C, Fujioka M, Mishima K, Irie K, Fujimura Y, Nakano T, Fandino J, Keller E, Iwasaki K, Fujiwara M Effect of ADAMTS-13 on cerebrovascular microthrombosis and neuronal injury after experimental subarachnoid hemorrhage. J Thromb Haemost, 2014-04-01;12(4):505-14. 2014-04-01 [PMID: 24476338] (Mouse)
• Kraisin S, Palasuwan A, Popruk S, Nantakomol D Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model. Malar J, 2014-01-03;13(0):3. 2014-01-03 [PMID: 24386898] (Human)

FAQs for ADAMTS13 (DADT130). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ADAMTS13