Thrombomodulin/BDCA-3 Antibody [Unconjugated]

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Thrombomodulin/BDCA‑3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Thrombomodulin/BDCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3894) at 10 µg/mL for ...read more
Thrombomodulin/BDCA‑3 was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Goat Anti-Mouse Thrombomodulin/BDCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Thrombomodulin/BDCA-3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Thrombomodulin/BDCA-3
Leu17-Ser517
Accession # P15306
Specificity
Detects mouse Thrombomodulin/BDCA-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
2% cross‑reactivity with recombinant human Thrombomodulin is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
THBD
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF3894 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thrombomodulin/BDCA-3 Antibody [Unconjugated]

  • BDCA3
  • BDCA-3
  • CD141 antigen
  • CD141
  • Fetomodulin
  • THBD
  • THRM
  • Thrombomodulin
  • TM

Background

Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid sequence of mouse THBD predicts a signal peptide (aa 1 to 16) and a mature chain (aa 17 to 577) that consists of the following domains: C-type lectin (aa 31 to 167), EGF-like (aa 240 to 280, aa 283 to 323, aa 324 to 362, aa 364 to 404, aa 405 to 439, and aa 440 to 480), transmembrane (aa 518 to 541) and cytoplasmic (aa 542 to 577) (1). The R&D Systems recombinant mouse THBD consists of aa 17 to 517, corresponding to the extracellular portion of the type I membrane protein.

Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (2‑4). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal amino acid residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (5). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (6).

  1. Dittman, W.A. and P.W. Majerus (1989) Nucleic Acids Res. 17:802.
  2. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
  3. Wu, K.K. et al. (2000) Ann Med. 32:73.
  4. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
  5. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
  6. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thrombomodulin/BDCA-3 Antibody (AF3894)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow Cytometry, IHC, IHC-P, Neutralization.


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Flow Cytometry
(1)
IHC
(1)
IHC-P
(3)
Neutralization
(1)
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Mouse
(6)
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Showing Publications 1 - 6 of 6.
Publications using AF3894 Applications Species
B Fields, A DeLaForest, M Zogg, J May, C Hagen, K Komnick, J Wieser, A Lundberg, H Weiler, MA Battle, KS Carlson The Adult Murine Intestine is Dependent on Constitutive Laminin-gamma1 Synthesis Sci Rep, 2019;9(1):19303. 2019 [PMID: 31848396] (IHC-P, Mouse) IHC-P Mouse
XY Liu, HX Xu, JK Li, D Zhang, XH Ma, LN Huang, JH Lü, XZ Wang Neferine Protects Endothelial Glycocalyx via Mitochondrial ROS in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome Front Physiol, 2018;9(0):102. 2018 [PMID: 29520236] (IHC-P, Mouse) IHC-P Mouse
BM Nagy, C Nagaraj, B Egemnazaro, G Kwapiszews, RE Stauber, A Avian, H Olschewski, A Olschewski Lack of ABCG2 Leads to Biventricular Dysfunction and Remodeling in Response to Hypoxia Front Physiol, 2017;8(0):98. 2017 [PMID: 28270772] (IHC, Mouse) IHC Mouse
Greineder, Colin F, Chacko, Ann-Mari, Zaytsev, Sergei, Zern, Blaine J, Carnemolla, Ronald, Hood, Elizabet, Han, Jingyan, Ding, Bi-Sen, Esmon, Charles, Muzykantov, Vladimir Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor. PLoS ONE, 2013;8(11):e80110. 2013 [PMID: 24244621] (Neutralization, Mouse) Neutralization Mouse
The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis. Nat. Med., 2012;18(0):1217. 2012 [PMID: 22820644] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Graham BB, Mentink-Kane MM, El-Haddad H Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling. Am. J. Pathol., 2010;177(3):1549-61. 2010 [PMID: 20671265] (IHC-P, Mouse) IHC-P Mouse

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF3894

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Blogs on Thrombomodulin/BDCA-3.

Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation
Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the...  Read full blog post.

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Bioinformatics

Gene Symbol THBD
Uniprot