Thrombomodulin/BDCA-3 Antibody [Unconjugated]


Thrombomodulin/BDCA‑3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Thrombomodulin/BDCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3894) at 10 µg/mL for more
Thrombomodulin/BDCA‑3 was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Goat Anti-Mouse Thrombomodulin/BDCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, ICC/IF

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Thrombomodulin/BDCA-3 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse Thrombomodulin/BDCA-3
Accession # P15306
Detects mouse Thrombomodulin/BDCA-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
2% cross‑reactivity with recombinant human Thrombomodulin is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF3894 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thrombomodulin/BDCA-3 Antibody [Unconjugated]

  • BDCA3
  • BDCA-3
  • CD141 antigen
  • CD141
  • Fetomodulin
  • THBD
  • THRM
  • Thrombomodulin
  • TM


Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid sequence of mouse THBD predicts a signal peptide (aa 1 to 16) and a mature chain (aa 17 to 577) that consists of the following domains: C-type lectin (aa 31 to 167), EGF-like (aa 240 to 280, aa 283 to 323, aa 324 to 362, aa 364 to 404, aa 405 to 439, and aa 440 to 480), transmembrane (aa 518 to 541) and cytoplasmic (aa 542 to 577) (1). The R&D Systems recombinant mouse THBD consists of aa 17 to 517, corresponding to the extracellular portion of the type I membrane protein.

Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (2‑4). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal amino acid residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (5). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (6).

  1. Dittman, W.A. and P.W. Majerus (1989) Nucleic Acids Res. 17:802.
  2. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
  3. Wu, K.K. et al. (2000) Ann Med. 32:73.
  4. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
  5. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
  6. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
Species: Ca, Fe, Hu, Mu
Applications: Dual ISH-IHC, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: ELISA
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IP, WB
Species: Hu, Rt
Applications: ELISA, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: IHC, WB
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: BA

Publications for Thrombomodulin/BDCA-3 Antibody (AF3894)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow Cytometry, IHC, IHC-P, Neutralization.

Filter By Application
Flow Cytometry
All Applications
Filter By Species
All Species
Showing Publications 1 - 6 of 6.
Publications using AF3894 Applications Species
B Fields, A DeLaForest, M Zogg, J May, C Hagen, K Komnick, J Wieser, A Lundberg, H Weiler, MA Battle, KS Carlson The Adult Murine Intestine is Dependent on Constitutive Laminin-gamma1 Synthesis Sci Rep, 2019;9(1):19303. 2019 [PMID: 31848396] (IHC-P, Mouse) IHC-P Mouse
XY Liu, HX Xu, JK Li, D Zhang, XH Ma, LN Huang, JH Lü, XZ Wang Neferine Protects Endothelial Glycocalyx via Mitochondrial ROS in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome Front Physiol, 2018;9(0):102. 2018 [PMID: 29520236] (IHC-P, Mouse) IHC-P Mouse
BM Nagy, C Nagaraj, B Egemnazaro, G Kwapiszews, RE Stauber, A Avian, H Olschewski, A Olschewski Lack of ABCG2 Leads to Biventricular Dysfunction and Remodeling in Response to Hypoxia Front Physiol, 2017;8(0):98. 2017 [PMID: 28270772] (IHC, Mouse) IHC Mouse
Greineder , Colin F, Chacko , Ann-Mari, Zaytsev , Sergei, Zern , Blaine J, Carnemolla , Ronald, Hood , Elizabet, Han , Jingyan, Ding , Bi-Sen, Esmon , Charles, Muzykantov , Vladimir Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor. PLoS ONE, 2013;8(11):e80110. 2013 [PMID: 24244621] (Neutralization, Mouse) Neutralization Mouse
The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis. Nat. Med., 2012;18(0):1217. 2012 [PMID: 22820644] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Graham BB, Mentink-Kane MM, El-Haddad H Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling. Am. J. Pathol., 2010;177(3):1549-61. 2010 [PMID: 20671265] (IHC-P, Mouse) IHC-P Mouse

Reviews for Thrombomodulin/BDCA-3 Antibody (AF3894) (0)

There are no reviews for Thrombomodulin/BDCA-3 Antibody (AF3894). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Thrombomodulin/BDCA-3 Antibody (AF3894) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Thrombomodulin/BDCA-3 Products

Bioinformatics Tool for Thrombomodulin/BDCA-3 Antibody (AF3894)

Discover related pathways, diseases and genes to Thrombomodulin/BDCA-3 Antibody (AF3894). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Thrombomodulin/BDCA-3 Antibody (AF3894)

Discover more about diseases related to Thrombomodulin/BDCA-3 Antibody (AF3894).

Pathways for Thrombomodulin/BDCA-3 Antibody (AF3894)

View related products by pathway.

PTMs for Thrombomodulin/BDCA-3 Antibody (AF3894)

Learn more about PTMs related to Thrombomodulin/BDCA-3 Antibody (AF3894).

Blogs on Thrombomodulin/BDCA-3.

Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation
Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Thrombomodulin/BDCA-3 Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol THBD