t-Plasminogen Activator/tPA Antibody

Images

 
Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - tPA antibody detects PLAT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGE tPA antibody dilution: 1:1000 (Rat)
Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Paraffin-embedded colon carcinoma. tPA antibody dilution: 1:250.
Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody diluted at 1:2000.
Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - tPA antibody detects PLAT protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract 7.5 % SDS-PAGE dilution: 1:1000 (Mouse)
Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Paraffin-embedded human hepatoma. tPA antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.15 mg/ml

Order Details

t-Plasminogen Activator/tPA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human tPA. The exact sequence is proprietary.
Localization
Secreted; extracellular space
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
Use in ELISA reported in scientific literature (PMID 28132883).
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-20648 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Porcine (82%), Bovine (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.15 mg/ml
Purity
Immunogen affinity purified

Alternate Names for t-Plasminogen Activator/tPA Antibody

  • Alteplase
  • DKFZp686I03148
  • EC 3.4.21
  • EC 3.4.21.68
  • plasminogen activator, tissue type
  • plasminogen activator, tissue
  • PLAT
  • Reteplase
  • tissue plasminogen activator (t-PA)
  • tissue-type plasminogen activator
  • TPA
  • T-PA
  • tPlasminogen Activator
  • t-Plasminogen Activator

Background

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for t-Plasminogen Activator/tPA Antibody (NBP2-20648)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA.


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ELISA
(1)
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Bioinformatics

Gene Symbol PLAT
Entrez
Uniprot