Von Willebrand Factor Antibody (8O6D7)

Western Blot: Von Willebrand Factor Antibody (8O6D7) [NBP3-15465] - Western blot analysis of extracts of Mouse liver, using von Willebrand factor (Von Willebrand Factor) Rabbit mAb (NBP3-15465) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (8O6D7) [NBP3-15465] - Immunohistochemistry of paraffin-embedded human esophageal using von Willebrand factor (Von Willebrand Factor) Rabbit mAb (NBP3-15465) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 8O6D7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Von Willebrand Factor Antibody (8O6D7) Summary

• Description: Novus Biologicals Rabbit Von Willebrand Factor Antibody (8O6D7) (NBP3-15465) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1150-1300 of human Von Willebrand Factor (P04275). APACQVTCQHPEPLACPVQCVEGCHAHCPPGKILDELLQTCVDPEDCPVCEVAGRRFASGKKVTLNPSDPEHCQICHCDVVNLTCEACQEPGGLVVPPTDAPVSPTTLYVEDISEPPLHDFYCSRLLDLVFLLDGSSRLSEAEFEVLKAFV
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VWF
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Von Willebrand Factor Antibody (8O6D7)

• coagulation factor VIII VWF
• F8
• F8VWF
• von Willebrand factor
• VWD
• vWF

Background

The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VWF