VEGFR2/KDR/Flk-1 Antibody

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody [NB100-686] - Formalin fixed paraffin embedded human angiosarcoma stained with Flk-1 antibody (NB100-686).

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

VEGFR2/KDR/Flk-1 Antibody Summary

• Immunogen: A synthetic peptide derived from the C-terminus of the precursor form of the mouse Flk-1.
• Localization: Type I membrane protein. Cytoplasmic.
• Marker: Endothelial Cell Marker
• Specificity: This antibody reacts with a 180 kDa protein, known as Flk-1, KDR or vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a cell membrane receptor kinase. Flk-1 is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDR
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Affinity purified

Alternate Names for VEGFR2/KDR/Flk-1 Antibody

• CD309 antigen
• CD309
• EC 2.7.10
• EC 2.7.10.1
• Fetal liver kinase 1
• fetal liver kinase-1
• Flk1
• Flk-1
• FLK1tyrosine kinase growth factor receptor
• KDR
• kinase insert domain receptor (a type III receptor tyrosine kinase)
• Kinase insert domain receptor
• KRD1
• Ly73
• Protein-tyrosine kinase receptor flk-1
• soluble VEGFR2
• vascular endothelial growth factor receptor 2
• VEGF R2
• VEGFR
• VEGFR2
• VEGFR-2

Background

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signaling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR2/KDR/Flk-1 Antibody (NB100-686)(1)

Publication using NB100-686

• Ostalska-Nowicka D, Malinska A, Zabel M et al. Nephrotic syndrome unfavorable course correlates with downregulation of podocyte vascular endothelial growth factor receptor (VEGFR)-2. Folia Histochem Cytobiol;49(3):472-8. 2011-01-01 [PMID: 22038228] (Human)

Bioinformatics

• Gene Symbol: KDR
• Entrez:
  • 3791(Human)
  • 16542(Mouse)
  • 25589(Rat)
• Uniprot:
  • P35918()