VEGFR2/KDR/Flk-1 Antibody

Images

 
Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-530] - Detection of VEGF R2/KDR/Flk-1 doublet in VEGF R2/KDR/Flk-1 induced HUVEC lysate (50 ug) using NB100-530 (1 ug/mL). ECL detection 1 minute.
Immunocytochemistry/ Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody [NB100-530] - V6.5 mouse embryonic stem cells (cat# NBP1-41162) stained with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more
Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-530] - Detection of VEGF R2/KDR/Flk-1 doublet in CSF-1 receptor/VEGF R2/KDR/Flk-1 chimera transfected lysate (20 ug) using NB100-530 (0.5 ug/mL). ECL detection 10 seconds.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.1 mg/ml

VEGFR2/KDR/Flk-1 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of the mouse VEGF Receptor 2 protein (between residues 1300-1367). [Swiss-Prot# P35918]
Localization
Type 1 membrane protein
Marker
Endothelial Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250 - 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry
Application Notes
In Western blot a doublet is seen at ~150 kDa. In ICC/IF nuclear and membrane staining can be seen in V6.5 mouse embryonic stem cells (Catalog No. NBP1-41162). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW reported in scientific literature (PMID: 29873146). Use in Immunohistochemistry reported in scientific literature (PMID: 30941660).
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
VEGFR2/KDR/Flk-1 Overexpression Lysate
v6.5 Mouse embryonic stem cells
Publications
Read Publications using
NB100-530 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28183816).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VEGFR2/KDR/Flk-1 Antibody

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGFs such as VEGFA, VEGFC, VEGFD etc. activate RTKs namely VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4), that regulates angiogenic as well as lymphangiogenic transduction signals. VEGF Receptor 2 (VEGFR2) is the most prominent cell-surface receptor for VEGF ligands and binding of VEGFs to isoform 1 leads to multiple signaling cascades activation. PLCG1 activation results in production of signaling molecules (diacylglycerol, inositol 1,4,5-trisphosphate etc) and PKC activation. VEGFR2 is involved in the activation of MAPK1/ERK2, MAPK3/ERK1, and MAPK as well as AKT signaling pathways. It facilitates phosphorylation of PIK3R1 (regulatory subunit of PI3K), cytoskeletal actin reorganization and PTK2/FAK1 activation. VEGFR2 is essential for VEGFA-mediated induction of NOS2/NOS3, leading to the production of NO by endothelial cells and is involved in phosphorylation of FYN, NCK1, NOS3, PIK3R1 and SRC. Isoforms -2 and -3 function as decoy receptors for VEGFs, and isoform -2 plays as negative regulator of VEGFA/VEGFC-mediated lymphangiogenesis by limiting free VEGFA and/or VEGFC levels alongwith preventing their binding to VEGFR3. Versatile signaling effects by VEGFR2 leads to its regulatory role in processes such as angiogenesis, vascular development, vascular permeability, embryonic hematopoiesis, and promotes proliferation, survival, migration and differentiation of endothelial cells alongwith facilitating reorganization of the actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR2/KDR/Flk-1 Antibody (NB100-530)(3)

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Product General Protocols

View specific protocols for VEGFR2/KDR/Flk-1 Antibody (NB100-530): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VEGFR2/KDR/Flk-1 Antibody (NB100-530) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-530AF350
Alexa Fluor 405 NB100-530AF405
Alexa Fluor 488 NB100-530AF488
Alexa Fluor 532 NB100-530AF532
Alexa Fluor 594 NB100-530AF594
Alexa Fluor 647 NB100-530AF647
Alexa Fluor 700 NB100-530AF700
Alexa Fluor 750 NB100-530AF750
Biotin NB100-530B
DyLight 350 NB100-530UV
DyLight 405 NB100-530V
DyLight 488 NB100-530G
DyLight 550 NB100-530R
DyLight 594 NB100-530DL594
DyLight 650 NB100-530C
DyLight 680 NB100-530FR
DyLight 755 NB100-530IR
FITC NB100-530F
HRP NB100-530H
Janelia Fluor 549 NB100-530JF549
Janelia Fluor 646 NB100-530JF646

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Bioinformatics Tool for VEGFR2/KDR/Flk-1 Antibody (NB100-530)

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Diseases for VEGFR2/KDR/Flk-1 Antibody (NB100-530)

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Bioinformatics

Gene Symbol KDR
Entrez