VEGF Antibody [Unconjugated] - 164


Western blot shows 25 ng of Recombinant Human VEGF165 (Catalog # 293-VE), Recombinant Mouse VEGF164 (Catalog # 493-MV), Recombinant Mouse VEGF120 (Catalog # 494-VE), Recombinant Mouse VEGF‑B167 (Catalog # more
Vascular Endothelial Growth Factor 164 (VEGF164) was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse VEGF164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA) at 15 more
Recombinant Mouse VEGF164 (Catalog # 493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by more

Product Details

Reactivity MuSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap)

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VEGF Antibody [Unconjugated] - 164 Summary

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF164
Accession # AAA40547
Detects mouse VEGF in ELISAs and Western blots. In Western blots, detection of recombinant mouse (rm) VEGF164, rmVEGF120, rmVEGF188, and recombinant rat VEGF164 is observered but no cross-reactivity with rmVEGF-B167,rmVEGF-B186 , or recombinant human (rh) VEGF165 is observed. In sandwich immunoassays, less than 0.04% cross-reactivity with rhVEGF121, rhVEGF165, and rhVEGF/PlGF is observed.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization <0.15 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse VEGF164 Antibody (Catalog # AF-493-NA)
ELISA Detection: Mouse VEGF Biotinylated Antibody (Catalog # BAF493)
Standard:Recombinant Mouse VEGF164 (Catalog # 493-MV)
Reviewed Applications
Read 2 Reviews rated 3.5
AF-493-NA in the following applications:

Read Publications using
AF-493-NA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF Antibody [Unconjugated] - 164

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor


Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Mouse VEGF164 is an approximately 50 kDa molecular weight homodimer sharing 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 90% with feline, equine and canine, and 88% with bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGF Antibody (AF-493-NA)(86)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 14 applications: Blocking, ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), Functional Assay, ICC, IHC, IHC-Fr, IHC-P, In Vivo, Luminex Development, Neutralization, Western Blot.

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Showing Publications 1 - 10 of 86. Show All 86 Publications.
Publications using AF-493-NA Applications Species
J Huang, Y Mao, Q Li, H Hong, N Tang, X Kang, Y Huang, J Liu, Q Gong, Y Yao, L Li Kallistatin prevents ovarian hyperstimulation syndrome by regulating vascular leakage Journal of Cellular and Molecular Medicine, 2022;26(16):4613-4623. 2022 [PMID: 35866203] (Neutralization, Mouse) Neutralization Mouse
J Fu, T Shinjo, Q Li, R St-Louis, K Park, MG Yu, H Yokomizo, F Simao, Q Huang, IH Wu, GL King Regenerating glomerular metabolism and function by podocyte pyruvate kinase M2 in diabetic nephropathy JCI Insight, 2022;0(0):. 2022 [PMID: 35133981] (Neutralization, Mouse) Neutralization Mouse
LJ Fan, HM Kan, XT Chen, YY Sun, LP Chen, W Shen Vascular endothelial growth factor-A/vascular endothelial growth factor2 signaling in spinal neurons contributes to bone cancer pain Molecular pain, 2022;18(0):1744806922107. 2022 [PMID: 35083936] (IHC, Mouse) IHC Mouse
A Yaguchi, M Oshikawa, G Watanabe, H Hiramatsu, N Uchida, C Hara, N Kaneko, K Sawamoto, T Muraoka, I Ajioka Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration Nature Communications, 2021;12(1):6623. 2021 [PMID: 34799548] (ELISA Capture, Mouse) ELISA Capture Mouse
D Philipp, M Holthaus, V Basoah, K Pfannkuche, L Suhr, T Wahlers, A Paunel-Gör VEGF Contributes to Mesenchymal Stem Cell-Mediated Reversion of Nor1-Dependent Hypertrophy in iPS Cell-Derived Cardiomyocytes Stem Cells International, 2021;2021(0):8888575. 2021 [PMID: 33927770] (Western Blot, Mouse) Western Blot Mouse
N Díaz-Lezam, A Wolf, S Koch, AM Pfaller, J Biber, X Guillonnea, T Langmann, A Grosche PDGF Receptor Alpha Signaling Is Key for M�ller Cell Homeostasis Functions International Journal of Molecular Sciences, 2021;22(3):. 2021 [PMID: 33503976] (Neutralization, Mouse) Neutralization Mouse
S Li, M Liu, MH Do, C Chou, EG Stamatiade, BG Nixon, W Shi, X Zhang, P Li, S Gao, KJ Capistrano, H Xu, NV Cheung, MO Li Cancer immunotherapy via targeted TGF-&amp;beta signalling blockade in TH cells Nature, 2020;0(0):. 2020 [PMID: 33087933] (IHC, Mouse) IHC Mouse
PL Mulcrone, SKE Edwards, DN Petrusca, LS Haneline, J Delgado-Ca, GD Roodman Osteocyte Vegf-a contributes to myeloma-associated angiogenesis and is regulated by Fgf23 Sci Rep, 2020;10(1):17319. 2020 [PMID: 33057033] (Neutralization, Human) Neutralization Human
Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1 Cancer Cell Int, 2020;20(0):476. 2020 [PMID: 33024414] (Western Blot, Human) Western Blot Human
K Ichikawa, S Watanabe M, Y Minoshima, J Matsui, Y Funahashi Activated FGF2 signaling pathway in tumor vasculature is essential for acquired resistance to anti-VEGF therapy Sci Rep, 2020;10(1):2939. 2020 [PMID: 32076044] (Western Blot, Mouse) Western Blot Mouse
Show All 86 Publications.

Reviews for VEGF Antibody (AF-493-NA) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF-493-NA:
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Images Ratings Applications Species Date Details
reviewed by:
Anonymous -
WB Mouse 01/08/2015


ApplicationWestern Blot
Sample TestedSee PMID 23670941
reviewed by:
cynthia morimoto
IF Mouse 12/19/2014


Sample TestedPrimary mouse cortical astrocytes

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FAQs for VEGF Antibody (AF-493-NA). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Blogs on VEGF. Showing 1-10 of 22 blog posts - Show all blog posts.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit.  ...  Read full blog post.

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Recent Reviews


Anonymous -
Application: WB
Species: Mouse

cynthia morimoto
Application: IF
Species: Mouse


Gene Symbol VEGFA