VCAM-1/CD106 Antibody [Unconjugated]

Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for VCAM-1/CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VCAM-1/CD106 was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Mouse bone marrow cells were stained with Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643, filled histogram) or control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107).

Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and 3T3-L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM-1/CD106 at approximately 116 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody ( HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

bEND.3 cells were stained with Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Markers of endothelial dysfunction in aorta.Expression of endoglin (A, B), eNOS (C, D), ICAM-1 (E, F) and VCAM-1 (G, H) in total protein extracts from mice aorta. Equal loading of samples was confirmed by immunodetection of GADPH (A, B). Top: densitometric analysis (control = 100%). Bottom: representative immunoblots. Data are shown as mean ± S.E.M. Mann-Whitney test, unpaired t-test. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119665), licensed under a CC-BY license. Not internally tested by R&D Systems.

Markers of endothelial dysfunction in aorta.Expression of endoglin (A, B), eNOS (C, D), ICAM-1 (E, F) and VCAM-1 (G, H) in total protein extracts from mice aorta. Equal loading of samples was confirmed by immunodetection of GADPH (A, B). Top: densitometric analysis (control = 100%). Bottom: representative immunoblots. Data are shown as mean ± S.E.M. Mann-Whitney test, unpaired t-test. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119665), licensed under a CC-BY license. Not internally tested by R&D Systems.

Markers of endothelial dysfunction in aorta.Expression of endoglin (A, B), eNOS (C, D), ICAM-1 (E, F) and VCAM-1 (G, H) in total protein extracts from mice aorta. Equal loading of samples was confirmed by immunodetection of GADPH (A, B). Top: densitometric analysis (control = 100%). Bottom: representative immunoblots. Data are shown as mean ± S.E.M. Mann-Whitney test, unpaired t-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25768936), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

VCAM-1/CD106 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse VCAM‑1/CD106
  Phe25-Glu698
  Accession # P29533
• Specificity: Detects mouse VCAM-1/CD106 in direct ELISAs and Western blots. In direct ELISA and Western blots, approximately 5% cross-reactivity with recombinant human VCAM-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VCAM1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VCAM-1/CD106 Antibody [Unconjugated]

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta , IL-4, TNF-alpha and IFN-gamma . VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7 . The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (AF643)(53)

Publications using AF643

• Johnson, CF;Schafer, CM;Burge, KY;Coon, BG;Chaaban, H;Griffin, CT; Endothelial RIPK3 minimizes organotypic inflammation and vascular permeability in ischemia-reperfusion injury bioRxiv : the preprint server for biology 2024-11-26 [PMID: 39651150] (Immunohistochemistry, Mouse)
• Muto, Y;Yoshimura, Y;Wu, H;Chang-Panesso, M;Ledru, N;Woodward, OM;Outeda, P;Cheng, T;Mahjoub, MR;Watnick, TJ;Humphreys, BD; Multi-omics profiling of mouse polycystic kidney disease progression at a single cell resolution bioRxiv : the preprint server for biology 2024-05-31 [PMID: 38854144] (Immunohistochemistry, Mouse)
• Manandhar, S;Gaddam, RR;Chambers, S;Bhatia, M; Kupffer Cell Inactivation Alters Endothelial Cell Adhesion Molecules in Cecal Ligation and Puncture-Induced Sepsis Biomolecules 2024-01-09 [PMID: 38254684] (IHC, Mouse)
• Manandhar, S;Chambers, S;Miller, A;Ishii, I;Bhatia, M; Pharmacological Inhibition and Genetic Deletion of Cystathionine Gamma-Lyase in Mice Protects against Organ Injury in Sepsis: A Key Role of Adhesion Molecules on Endothelial Cells International journal of molecular sciences 2023-09-04 [PMID: 37686458] (IHC, Transgenic Mouse, Mouse)
• Yu, L;Xu, L;Chu, H;Peng, J;Sacharidou, A;Hsieh, HH;Weinstock, A;Khan, S;Ma, L;Durán, JGB;McDonald, J;Nelson, ER;Park, S;McDonnell, DP;Moore, KJ;Huang, LJ;Fisher, EA;Mineo, C;Huang, L;Shaul, PW; Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice Nature communications 2023-07-25 [PMID: 37491347] (IHC, Mouse)
• Anderson R. A. Cerqueira, Leandro Rodrigues, Silvia Abigail Coavoy-Sánchez, Simone A. Teixeira, Karla B. Feitosa, Erika Y. Taniguchi et al. Aedes aegypti salivary gland extract alleviates acute itching by blocking TRPA1 channels Frontiers in Physiology 2023-06-27 [PMID: 37441000]
• Zhang, X;Li, S;Malik, I;Do, MH;Ji, L;Chou, C;Shi, W;Capistrano, KJ;Zhang, J;Hsu, TW;Nixon, BG;Xu, K;Wang, X;Ballabio, A;Schmidt, LS;Linehan, WM;Li, MO; Reprogramming tumour-associated macrophages to outcompete cancer cells Nature 2023-06-28 [PMID: 37380769] (IHC, Mouse)
• Kim, JY;Yi, MH;Kim, M;Choi, JH;Lee, S;Yong, TS; Production of Dermatophagoides farinae Having Low Bacterial Content Using Ampicillin Journal of immunology research 2023-05-18 [PMID: 37252681] (Immunohistochemistry, Mouse)
• Hyun-Jin An, Mi-Gyeong Gwon, Hyemin Gu, Seongjae Bae, Jaechan Leem, Jin Bae Lee et al. STAT3/NF‑ kappa B decoy oligodeoxynucleotides inhibit atherosclerosis through regulation of the STAT/NF‑ kappa B signaling pathway in a mouse model of atherosclerosis International Journal of Molecular Medicine 2023-03-31 [PMID: 37026512]
• Rebeca Uceda-Castro, Andreia S. Margarido, Lesley Cornet, Serena Vegna, Kerstin Hahn, Ji-Ying Song et al. Re-purposing the pro-senescence properties of doxorubicin to introduce immunotherapy in breast cancer brain metastasis Cell Reports Medicine 11/15/2022 [PMID: 36384097]
• Minzhi Yu, Kristen Hong, Reheman Adili, Ling Mei, Lisha Liu, Hongliang He et al. Development of activated endothelial targeted high-density lipoprotein nanoparticles Frontiers in Pharmacology 2022-08-29 [PMID: 36105190]
• Catherine S. Coleman, Bruce A. Stanley, Charles H. Lang Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-gamma Inflammation 2022-06-01 [PMID: 35028803]
• Philipp-Sebastian Koch, Kajetan Sandorski, Joschka Heil, Christian D. Schmid, Sina W. Kürschner, Johannes Hoffmann et al. Imbalanced Activation of Wnt-/ beta -Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation Frontiers in Physiology 2021-09-29 [PMID: 34658910] (Immunohistochemistry, Transgenic Mouse)
• Yecheng He, Huadong Li, Juan Yao, Hua Zhong, Yanbin Kuang, Xin Li et al. HO‑1 knockdown upregulates the expression of VCAM‑1 to induce neutrophil recruitment during renal ischemia‑reperfusion injury International Journal of Molecular Medicine 2021-08-03 [PMID: 34368855] (Western Blot, In vivo assay, Neutralization, Mouse)
• Minshu Wang, Colin E. Murdoch, Alison C. Brewer, Aleksandar Ivetic, Paul Evans, Ajay M. Shah et al. Endothelial NADPH oxidase 4 protects against angiotensin II‐induced cardiac fibrosis and inflammation ESC Heart Failure 2021-04-01 [PMID: 33511759]
• K Sato, A Tobo, C Mogi, M Tobo, N Yamane, M Tosaka, H Tomura, DS Im, F Okajima The protective role of proton-sensing TDAG8 in the brain injury in a mouse ischemia reperfusion model Sci Rep, 2020-10-14;10(1):17193. 2020-10-14 [PMID: 33057165] (Western Blot, Mouse)
• SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm Exp. Mol. Med., 2020-09-14;0(0):. 2020-09-14 [PMID: 32929220] (Western Blot, Mouse)
• Marcus Ruscetti, John P. Morris, Riccardo Mezzadra, James Russell, Josef Leibold, Paul B. Romesser et al. Senescence-Induced Vascular Remodeling Creates Therapeutic Vulnerabilities in Pancreas Cancer Cell 2020-04-01 [PMID: 32234521]
• S Colijn, V Muthukumar, J Xie, S Gao, CT Griffin Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis Dis Model Mech, 2020-01-24;13(1):. 2020-01-24 [PMID: 31953345] (Western Blot, Mouse)
• Hyehun Choi, Ryan J. Stark, Benjamin S. Raja, Anna Dikalova, Fred S. Lamb Apoptosis signal-regulating kinase 1 activation by Nox1-derived oxidants is required for TNF alpha receptor endocytosis American Journal of Physiology-Heart and Circulatory Physiology 2019-06-01 [PMID: 30925081]
• Xiaoyu Liu, Daniel P. Nemeth, Daniel B. McKim, Ling Zhu, Damon J. DiSabato, Olimpia Berdysz et al. Cell-Type-Specific Interleukin 1 Receptor 1 Signaling in the Brain Regulates Distinct Neuroimmune Activities Immunity 2019-02-01 [PMID: 30683620]
• P Wohlrab, L Soto-Gonza, T Benesch, MP Winter, IM Lang, K Markstalle, V Tretter, KU Klein Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells Front Physiol, 2018-12-06;9(0):1754. 2018-12-06 [PMID: 30574096] (Western Blot, Mouse)
• FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus Elife, 2018-09-06;7(0):. 2018-09-06 [PMID: 30187863] (IHC, Mouse)
• JA Giles, AD Greenhalgh, A Denes, B Nieswandt, G Coutts, BW McColl, SM Allan Neutrophil infiltration to the brain is platelet-dependent, and is reversed by blockade of platelet GPIb? Immunology, 2018-02-08;0(0):. 2018-02-08 [PMID: 29325217] (IHC, Mouse)
• Y Xu, S Xu, P Liu, M Koroleva, S Zhang, S Si, ZG Jin Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis J Am Heart Assoc, 2017-11-30;6(12):. 2017-11-30 [PMID: 29191808] (Western Blot, Mouse)
• Xiaoyu Li, Karen L. Kover, Daniel P. Heruth, Dara J. Watkins, Yanchun Guo, Wayne V. Moore et al. Thioredoxin-interacting protein promotes high-glucose-induced macrovascular endothelial dysfunction Biochemical and Biophysical Research Communications 2017-11-01 [PMID: 28890350]
• N Akbar, JE Digby, TJ Cahill, AN Tavare, AL Corbin, S Saluja, S Dawkins, L Edgar, N Rawlings, K Ziberna, E McNeill, E Johnson, AA Aljabali, RA Dragovic, M Rohling, TG Belgard, IA Udalova, DR Greaves, KM Channon, PR Riley, DC Anthony, RP Choudhury Endothelium-derived extracellular vesicles promote splenic monocyte mobilization in myocardial infarction JCI Insight, 2017-09-07;2(17):. 2017-09-07 [PMID: 28878126] (Cell Selection, Mouse)
• J Griger, R Schneider, I Lahmann, V Schöwel, C Keller, S Spuler, M Nazaré, C Birchmeier Loss of Ptpn11 (Shp2) drives satellite cells into quiescence Elife, 2017-05-02;6(0):. 2017-05-02 [PMID: 28463680] (IHC, Mouse)
• CC Wei, YY Kong, GQ Li, YF Guan, P Wang, CY Miao Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway Sci Rep, 2017-04-06;7(1):717. 4/6/2017 [PMID: 28386082]
• Mark Hepokoski, Joshua A. Englert, Rebecca M. Baron, Laura E. Crotty-Alexander, Mark M. Fuster, Jeremy R. Beitler et al. Ventilator-induced lung injury increases expression of endothelial inflammatory mediators in the kidney American Journal of Physiology-Renal Physiology 2017-04-01 [PMID: 28365585]
• YT Lin, L Barske, T DeFalco, B Capel Numb regulates somatic cell lineage commitment during early gonadogenesis in mice Development, 2017-03-30;0(0):. 2017-03-30 [PMID: 28360133] (IHC, Mouse)
• Edward J. Sanderlin, Nancy R. Leffler, Kvin Lertpiriyapong, Qi Cai, Heng Hong, Vasudevan Bakthavatchalu et al. GPR4 deficiency alleviates intestinal inflammation in a mouse model of acute experimental colitis Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2017-02-01 [PMID: 27940273]
• Hyehun Choi, Nicholas Ettinger, Jeffrey Rohrbough, Anna Dikalova, Hong N. Nguyen, Fred S. Lamb LRRC8A channels support TNF alpha -induced superoxide production by Nox1 which is required for receptor endocytosis Free Radical Biology and Medicine 2016-12-01 [PMID: 27838438]
• Di Cui, Houxuan Li, Lang Lei, Changxing Chen, Fuhua Yan Nonsurgical periodontal treatment reduced aortic inflammation in ApoE(-/-) mice with periodontitis SpringerPlus 2016-12-01 [PMID: 27386384]
• Park K, Mima A, Li Q et al. Insulin decreases atherosclerosis by inducing endothelin receptor B expression. JCI Insight. 5/5/2016 [PMID: 27200419]
• Ine Vandersmissen, Sander Craps, Maarten Depypere, Giulia Coppiello, Nick van Gastel, Frederik Maes et al. Endothelial Msx1 transduces hemodynamic changes into an arteriogenic remodeling response Journal of Cell Biology 2015-09-28 [PMID: 26391659] (Immunohistochemistry, Human, Mouse)
• Cedervall J, Zhang Y, Huang H, Zhang L, Femel J, Dimberg A, Olsson A Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals. Cancer Res, 2015-06-12;75(13):2653-62. 2015-06-12 [PMID: 26071254]
• Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw T, Boyce J Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors. J Immunol, 2015-04-22;194(11):5061-8. 2015-04-22 [PMID: 25904552] (Western Blot, Mouse)
• Nemeckova I, Serwadczak A, Oujo B, Jezkova K, Rathouska J, Fikrova P, Varejckova M, Bernabeu C, Lopez-Novoa J, Chlopicki S, Nachtigal P High soluble endoglin levels do not induce endothelial dysfunction in mouse aorta. PLoS ONE, 2015-03-13;10(3):e0119665. 2015-03-13 [PMID: 25768936] (Western Blot, Mouse)
• Clement M, Guedj K, Andreata F, Morvan M, Bey L, Khallou-Laschet J, Gaston A, Delbosc S, Alsac J, Bruneval P, Deschildre C, Le Borgne M, Castier Y, Kim H, Cantor H, Michel J, Caligiuri G, Nicoletti A Control of the T follicular helper-germinal center B-cell axis by CD8(+) regulatory T cells limits atherosclerosis and tertiary lymphoid organ development. Circulation, 2014-12-31;131(6):560-70. 2014-12-31 [PMID: 25552357] (IHC, Mouse)
• Caroline M. Sawicki, Daniel B. McKim, Eric S. Wohleb, Brant L. Jarrett, Brenda F. Reader, Diana M. Norden et al. Social defeat promotes a reactive endothelium in a brain region-dependent manner with increased expression of key adhesion molecules, selectins and chemokines associated with the recruitment of myeloid cells to the brain Neuroscience 2015-08-27 [PMID: 25445193]
• Ferrer M, Shuvaev V, Zern B, Composto R, Muzykantov V, Eckmann D Icam-1 targeted nanogels loaded with dexamethasone alleviate pulmonary inflammation. PLoS ONE, 2014-07-14;9(7):e102329. 2014-07-14 [PMID: 25019304] (Western Blot, Mouse)
• Rampanelli , Elena, Dessing , Mark C, Claessen , Nike, Teske , Gwendoli, Joosten , Sander P, Pals , Steven T, Leemans , Jaklien, Florquin , Sandrine CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction. PLoS ONE, 2013-12-23;8(12):e84479. 2013-12-23 [PMID: 24376813] (IHC, Mouse)
• Bianca Hemmeryckx, Marc F. Hoylaerts, Eveline Deloose, Cor E. Van Hove, Paul Fransen, Hidde Bult et al. Age-associated pro-inflammatory adaptations of the mouse thoracic aorta Thrombosis and Haemostasis 2013-01-10 [PMID: 23925372]
• Bianca Hemmeryckx, Marijke Gaekens, David J. Gallacher, Hua Rong Lu, Henri Roger Lijnen Effect of Rosiglitazone on Liver Structure and Function in Genetically Diabetic Akita Mice Basic & Clinical Pharmacology & Toxicology 2013-07-01 [PMID: 23789962]
• Henry S. Cheng, Nirojini Sivachandran, Andrew Lau, Emilie Boudreau, Jimmy L. Zhao, David Baltimore et al. Micro RNA ‐146 represses endothelial activation by inhibiting pro‐inflammatory pathways EMBO Molecular Medicine 2013-07-01 [PMID: 23733368]
• Makiko Takeichi, Keisuke Nimura, Masaki Mori, Hironori Nakagami, Yasufumi Kaneda The Transcription Factors Tbx18 and Wt1 Control the Epicardial Epithelial-Mesenchymal Transition through Bi-Directional Regulation of Slug in Murine Primary Epicardial Cells PLoS ONE 2013-02-28 [PMID: 23469079]
• Ko IK, Kim BG, Awadallah A Targeting improves MSC treatment of inflammatory bowel disease. Mol. Ther., 2010-04-13;18(7):1365-72. 2010-04-13 [PMID: 20389289] (Neutralization, Mouse)
• Katagiri K, Katakai T, Ebisuno Y, Ueda Y, Okada T, Kinashi T Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes. EMBO J., 2009-04-02;28(9):1319-31. 2009-04-02 [PMID: 19339990] (IHC-Fr, Mouse)
• Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice. Circulation, 2007-12-24;117(3):421-8. 2007-12-24 [PMID: 18158360] (IHC-P, Mouse)
• Nguyen M, Pace AJ, Koller BH Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia. J. Exp. Med., 2007-05-21;204(6):1383-93. 2007-05-21 [PMID: 17517966] (Western Blot, Mouse)
• Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S High pressure promotes monocyte adhesion to the vascular wall. Circ. Res., 2007-03-29;100(8):1226-33. 2007-03-29 [PMID: 17395876] (IHC, Mouse)
• Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells. Blood, 2007-01-16;109(9):3706-12. 2007-01-16 [PMID: 17227831] (Neutralization, Mouse)

Reviews for VCAM-1/CD106 Antibody (AF643) (3)

Average Rating: 3.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Omer Aydin IHC Mouse 03/27/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: 4T1 mouse breast cancer cell line,4t1 tissue section
• Species: Mouse
• Lot: CMX061608

Comments

• Comments: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). 4T1 tissues were cut 5 um. After deparaffin steps, the section was pre-treated using heat mediated antigen retrieval with Citrate buffer @ pH 6.0 (C9999, Thermo-Scientific) solution for 20 min at 100 C. Protein blocked was processed with superblock (#37515, Thermo Scientific) for 30 min. Afterwards, the section was then incubated with VCAM-1 pAb with 100X dilution of recommended concentration (100ug/500uL in PBS) for 1h at room temperature. A donkey anti-goat biotinylated secondary antibody (IgG H&L #ab7125, 1/200) was used to detect the primary and visualized using an HRP system (30 min incubation). DAB (#SK-4105, Vector) was used as the chromogen. The section was then counterstained with methyl green (#H-3402, Vector) and mounted with Cytoseal 60.

reviewed by: Verified Customer IHC-Fr Mouse 01/09/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23021255
• Species: Mouse

reviewed by: Verified Customer IF Mouse 01/09/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23232501
• Species: Mouse

Bioinformatics

• Gene Symbol: VCAM1
• Uniprot:
  • P29533()