Integrin alpha L/CD11a Antibody (MEM-25)

Images

 
Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) [NB500-328] - Analysis using the PE conjugate of NB500-328. Surface staining of human peripheral blood cells with anti-human CD11a (MEM-25) PE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IP, CyTOF-ready
Clone
MEM-25
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Integrin alpha L/CD11a Antibody (MEM-25) Summary

Immunogen
Leucocytes from a patient suffering from a LGL-type leukemia (NM_001114380.1).
Specificity
The antibody MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. HLDA IV; WS Code NL 209
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGAL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2 ug/ml
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Purity is > 95% (by SDS-PAGE). Immunoprecipitation - excellent antibody for immunoaffinity purification of LFA-1 complex Functional Application - The antibody MEM-25 partially blocks binding of LFA-1 complex to ICAM-1.
Positive Control
Integrin alpha L/CD11a Lysate (NBL1-12068)
Publications
Read Publications using NB500-328.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Integrin alpha L/CD11a Antibody (MEM-25)

  • CD11 antigen-like family member A
  • CD11a antigen
  • CD11a
  • CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
  • Integrin alpha L
  • integrin alpha-L
  • integrin gene promoter
  • integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
  • ITGAL
  • Leukocyte adhesion glycoprotein LFA-1 alpha chain
  • Leukocyte function-associated molecule 1 alpha chain
  • LFA-1 alpha
  • LFA-1
  • LFA1A
  • LFA-1A
  • Ly-15
  • Ly-21
  • lymphocyte function-associated antigen 1

Background

CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha L/CD11a Antibody (NB500-328)(18)


Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB500-328 Applications Species
Aubert M, Yoon M, Sloan DD et al. The virological synapse facilitates herpes simplex virus entry into T cells J Virol 2009 Jun [PMID: 19339346]

Details:
This citation used the FITC version of this antibody.
Wang JH, Kwas C, Wu L. Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission J Virol 2009 May [PMID: 19211748]

Details:
This citation used the FITC version of this antibody.
Ottonello L, Epstein AL, Mancini M et al. Monoclonal LYM-1 antibody-dependent cytolysis by human neutrophils exposed to GM-CSF: auto-regulation of target cell attack by cathepsin G J Leukoc Biol 2004 Jan [PMID: 14525961]

Details:
This citation used the FITC version of this antibody.
Mathison RD, Befus AD, Davison JS, Woodman RC. Modulation of neutrophil function by the tripeptide feG BMC Immunol 2003 Mar [PMID: 12659660]

Details:
This citation used the FITC version of this antibody.
Hajishengallis G, Martin M, Sojar HT et al. Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction Clin Diagn Lab Immunol 2002 Mar [PMID: 11874886]

Details:
This citation used the FITC version of this antibody.
Garnotel R, Rittie L, Poitevin S et al. Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95) J Immunol 2000 Jun 1 [PMID: 10820275]

Details:
This citation used the FITC version of this antibody.
Simon SI, Cherapanov V, Nadra I et al. Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18 J Immunol 1999 Sep 1 [PMID: 10453036]

Details:
This citation used the FITC version of this antibody.
Ottonello L, Epstein AL, Dapino P et al. Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins Blood [PMID: 10233903]

Details:
Used the FITC form of this antibody.
Bazil V, Stefanova I, Hilgert I et al. Monoclonal antibodies against human leucocyte antigens IV Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein Folia Biol (Praha) 1990 [PMID: 1971601]

Details:
This citation used the FITC version of this antibody.
Ottonello L, Epstein AL, Mancini M et al. Monoclonal LYM-1 antibody-dependent cytolysis by human neutrophils exposed to GM-CSF: auto-regulation of target cell attack by cathepsin G J Leukoc Biol

Details:
This publication used the FITC conjugated form of this antibody (Cat# NB500-636).
Show All 18 Publications.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-328AF405
Alexa Fluor 488 Labeled NB500-328AF488
Alexa Fluor 647 Labeled NB500-328AF647
Alexa Fluor 700 Labeled NB500-328AF700
Biotin Labeled NB110-81625

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Bioinformatics

Gene Symbol ITGAL
Entrez