VCAM-1/CD106 Antibody (OTI3H10)

Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VCAM1 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VCAM1.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Human prostate tissue using anti-VCAM1 mouse monoclonal antibody.

Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VCAM1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - CD106 / VCAM1 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VCAM1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Human Kidney tissue using anti-VCAM1 mouse monoclonal antibody.

• Reactivity: Hu, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI3H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

VCAM-1/CD106 Antibody (OTI3H10) Summary

• Description: Novus Biologicals Mouse VCAM-1/CD106 Antibody (OTI3H10) (NBP2-03600) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human VCAM1 (NP_001069) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:1000-2000
• Theoretical MW: 78.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VCAM-1/CD106 Antibody (OTI3H10)

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (NBP2-03600)(1)

Publication using NBP2-03600

• Martin CA Pathology of complicated Babesia rossi-associated acute lung injury and respiratory distress syndrome in dogs Thesis 2020-01-01

FAQs for VCAM-1/CD106 Antibody (NBP2-03600). (Showing 1 - 1 of 1 FAQ).

1. I am doing a research product that require investigation of endothelial activation in canine lungs. I see that your VCAM-1 does have reactivity in canines according to your website. Will it work in parafin embedded sections? 
   • Our VCAM-1 antibody with catalogue number NBP2-03600 is covered by our 100% quality guarantee for detection of the canine protein, and for IHC with paraffin-embedded tissue samples. You can read more about our quality guarantee at the following link: quality assurance.

Bioinformatics

• Gene Symbol: VCAM1
• Entrez:
  • 7412(Human)