VCAM-1/CD106 Antibody (B-K9 (C106-K9)) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (B-K9 (C106-K9)) - Chimeric [NBP3-12081] - Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of B-K9 (C106-K9) (NBP3-12081) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12081, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: VCAM-1/CD106 Antibody (B-K9 (C106-K9)) - Chimeric - Azide and BSA Free [NBP3-12081] - Paraformaldehyde fixed K562 cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12081 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: Flow, ICC/IF, B/N
• Clone: B-K9 (C106-K9)
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

VCAM-1/CD106 Antibody (B-K9 (C106-K9)) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunizing Balb/c mice against activated human umbilical vein endothelial cells (HUVEC).
• Specificity: B-K9 antibody recognizes VCAM-1/CD106 (VCAM1). VCAM-1/CD106 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VCAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for VCAM-1/CD106 Antibody (B-K9 (C106-K9)) - Chimeric - Azide and BSA Free

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VCAM1