VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182] - Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 antibody at 1:100 using peroxidase-conjugate and DAB chromogen. Note membrane staining.

ELISA: VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free [NBP2-33182] - Analysis using In-cell ELISA with HDMVEC cells.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 1.4C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29413).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Clone 1.4C3 was used by HLDA to establish CD designation.
• Immunogen: Stimulated human umbilical vein endothelial cells (HUVEC)
• Localization: Cell surface
• Marker: Activated Endothelial Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for VCAM-1/CD106 Antibody (1.4C3) - Azide and BSA Free

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (NBP2-33182)(1)

Publication using NBP2-33182

• Troupin A, Shirley D, Londono-Renteria B et al. A Role for Human Skin Mast Cells in Dengue Virus Infection and Systemic Spread. J Immunol. 2016-12-01 [PMID: 27799312]
  
  Details:
  This citation used the HRP version of this antibody.

Bioinformatics

• Gene Symbol: VCAM1