VCAM-1/CD106 Antibody (1.4C3) [HRP]

Western Blot: VCAM-1/CD106 Antibody (1.4C3) [HRP] [NBP2-33182H] - analysis of VCAM-1 in HDMVE cell lysate using anti-VCAM-1 antibody. Image from verified customer review.

ELISA: VCAM-1/CD106 Antibody (1.4C3) [HRP] [NBP2-33182H] - In-cell ELISA performed with HDMVE cells using HRP conjugated VCAM-1 antibody. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 1.4C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

VCAM-1/CD106 Antibody (1.4C3) [HRP] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Additional Information: Clone 1.4C3 was used by HLDA to establish CD designation.
• Immunogen: Stimulated human umbilical vein endothelial cells (HUVEC)
• Localization: Cell surface
• Marker: Activated Endothelial Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: ELISA reported by a customer review and from a citation (PMID:27799312). WB reported by a customer review.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for VCAM-1/CD106 Antibody (1.4C3) [HRP]

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (NBP2-33182H)(1)

Publication using NBP2-33182H

• Troupin A, Shirley D, Londono-Renteria B et al. A Role for Human Skin Mast Cells in Dengue Virus Infection and Systemic Spread. J Immunol. 2016-12-01 [PMID: 27799312] (ELISA, Human)
  
  Details:
  This citation used the HRP version of this antibody.

Reviews for VCAM-1/CD106 Antibody (NBP2-33182H) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 11/10/2015

Summary

• Application: Western Blot
• Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
• Species: Human

reviewed by: Verified Customer ELISA Human 11/10/2015

Summary

• Application: ELISA
• Sample Tested: Human Dermal Microvascular Endothelial cells - fixed cells used in In-cell ELISA
• Species: Human

Bioinformatics

• Gene Symbol: VCAM1