UCP2 Antibody [Unconjugated]

Western blot shows lysates of mouse brown adipose tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse UCP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human UCP1, UCP2, UCP3, and UCP4 (5 ng/lane) were included. A specific band for UCP2 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Simple Western lane view shows lysates of mouse brown adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for UCP2 at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse UCP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4739) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Adiponectin enhances the mRNA stability of UCP2 and promotes its protein synthesis.Two inhibitors, actinomycin D (ActD, A, B and C) or cycloheximide (CHX, A, B and D), were administered together with or without adiponectin protein into liver tissues of AKO mice livers. The protein (A) and mRNA (B) abundance of UCP2 was monitored by Western blotting and QPCR, respectively. The relative protein expression was also monitored in PCs/NPCs lysates (C and D). *, P<0.05 vs corresponding controls, n = 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.

Acute treatment with adiponectin elevates UCP2 expression in NPCs.PBS or 50 µg of murine adiponectin protein was injected into the portal vein of AKO mice livers. Liver tissues were collected for evaluation of UCP2 expression. The protein abundance in mitochondria (A), mRNA expression in total tissue lysates (B), and the protein content in PC and NPC fractions (C) were analyzed as in Figure 1. *, P<0.05 vs vehicle treated samples, n = 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.

Acute treatment with adiponectin elevates UCP2 expression in NPCs.PBS or 50 µg of murine adiponectin protein was injected into the portal vein of AKO mice livers. Liver tissues were collected for evaluation of UCP2 expression. The protein abundance in mitochondria (A), mRNA expression in total tissue lysates (B), and the protein content in PC and NPC fractions (C) were analyzed as in Figure 1. *, P<0.05 vs vehicle treated samples, n = 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.

Adiponectin promotes UCP2 expression in hepatic endothelial cells.AKO mice were treated as in Figure 1B. The NPCs were used for further fractionation to collect those enriched with Kupffer (K)- and sinusoidal endothelial (E) cells. The enrichment of the two cell types were confirmed by Western blotting using macrophage marker F4/80 and sinusoidal endothelial marker SE-1, respectively (A). UCP2 expression was monitored as in Figure 1. After densitometry analysis, the protein ratio of UCP2/ beta -actin was calculated and presented as fold changes against Luci Kupffer samples (B). UCP2 gene expression was also quantified in four types of cells treated with or without adiponectin (10 µg/ml) (C). *, P<0.05 and **, P<0.01 vs corresponding controls, n = 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

UCP2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human UCP2
  Met1-Phe309
  Accession # P55851
• Specificity: Detects human and mouse UCP2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) UCP1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UCP2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UCP2 Antibody [Unconjugated]

• mitochondrial uncoupling protein 2
• SLC25A8
• SLC25A8BMIQ4
• Solute carrier family 25 member 8
• UCP 2
• UCP2
• UCPH
• uncoupling protein 2 (mitochondrial, proton carrier)

Background

Mitochondrial Uncoupling Protein 2 (UCP2) is a 33 kDa member of the mitochondrial carrier protein family. UCP2 uncouples respiration from oxidative phosphorylation, principally in fat and skeletal muscle. Unlike UCP1, which generates heat in response to cold, UCP2 generates heat in response to dietary fluctuations. Human UCP2 is 309 amino acid (aa) in length. It contains six transmembrane domains (aa’s 11‑291) and is embedded in the inner mitochondrial membrane. Here, it dimerizes, forming a proton channel. There is one nucleotide binding site (aa 276‑298). Full‑length UCP2 shares 94%, 97% and 96% aa identity with porcine, canine and mouse UCP2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UCP2 Antibody (AF4739)(4)

Publications using AF4739

• Y Takeda, T Yoshikawa, P Dai Transcriptome analysis reveals brown adipogenic reprogramming in chemical compound-induced brown adipocytes converted from human dermal fibroblasts Scientific Reports, 2021-03-03;11(1):5061. 2021-03-03 [PMID: 33658606] (Western Blot, Human)
• Yang Zhang, Jian Liu, Jiang-Yun Luo, Xiao Yu Tian, Wai San Cheang, Jian Xu et al. Upregulation of Angiotensin (1-7)-Mediated Signaling Preserves Endothelial Function Through Reducing Oxidative Stress in Diabetes Antioxidants & Redox Signaling 10/10/2015 [PMID: 25867182]
• Rose , Shannon, Frye , Richard, Slattery , John, Wynne , Rebecca, Tippett , Marie, Pavliv , Oleksand, Melnyk , Stepan, James , S Jill Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort. PLoS ONE, 2014-01-08;9(1):e85436. 2014-01-08 [PMID: 24416410] (Western Blot, Human)
• Zhou M, Xu A, Tam PK, Lam KS, Huang B, Liang Y, Lee IK, Wu D, Wang Y. Upregulation of UCP2 by Adiponectin: The Involvement of Mitochondrial Superoxide and hnRNP K. PLoS One;7(2):e32349. 1/1/2012 [PMID: 22359684]

FAQs for UCP2 Antibody (AF4739). (Showing 1 - 1 of 1 FAQs).

1. Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.
   • Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.

Bioinformatics

• Gene Symbol: UCP2
• Uniprot:
  • P55851()