UCP3 Antibody

Western Blot: UCP3 Antibody [NBP2-24608] - Analysis of UCP3 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart

• Reactivity: Hu, Mu, Rt, Pm, Po, Ca
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UCP3 Antibody Summary

• Immunogen: A portion of amino acids 125-175 of human UCP3 was used as the immunogen.
• Predicted Species: Porcine (94%), Canine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UCP3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2.0 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 84% homologous in bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for UCP3 Antibody

• mitochondrial uncoupling protein 3
• SLC25A9Solute carrier family 25 member 9
• UCP 3
• uncoupling protein 3 (mitochondrial, proton carrier)
• Uncoupling protein-3

Background

The uncoupling proteins (UCPs) are found in the inner mitochondrial membranes and belong to a family of mitochondrial anion transport proteins that include the phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP 1 is abundantly expressed in brown adipose tissue of mammals. UCP 1 allows proton re-entry into the mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is thought that UCP 2 and 3 also dissipate the mitochondrial proton gradient produced by the respiratory chain in cells and tissues within which they are expressed. Studies have shown UCP 2 to be expressed by a variety of tissues. UCP 3 is expressed predominantly in brown adipose tissue, skeletal muscle, at lower levels in heart and smooth muscle, and is absent in liver and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UCP3 Antibody (NBP2-24608)(3)

Publications using NBP2-24608

• Luo Z, Huang J, Li Z et al. Cajanolactone A, a Stilbenoid From Cajanus canjan (L.) Millsp, Prevents High-Fat Diet-Induced Obesity via Suppressing Energy Intake Frontiers in Pharmacology 2021-05-31 [PMID: 34135763] (Western Blot, Mouse)
• Rupert JE, Narasimhan A, Jengelley DHA et al. Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia The Journal of experimental medicine 2021-06-07 [PMID: 33851955] (WB, Mouse)
• Sekerci G Sicanlara meteorin-like protein uygulamasının hipotalamus-hipofiz-tiroid aksı ve periferal uncoupling proteinler Uzerindeki etkileri Thesis 1905-07-13

Bioinformatics

• Gene Symbol: UCP3
• Uniprot:
  • P55916(Human)