UCP2 Antibody [Alexa Fluor® 647]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

UCP2 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human UCP2
  Met1-Phe309
  Accession # P55851
• Specificity: Detects human and mouse UCP2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) UCP1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UCP2 Antibody [Alexa Fluor® 647]

• mitochondrial uncoupling protein 2
• SLC25A8
• SLC25A8BMIQ4
• Solute carrier family 25 member 8
• UCP 2
• UCP2
• UCPH
• uncoupling protein 2 (mitochondrial, proton carrier)

Background

Mitochondrial Uncoupling Protein 2 (UCP2) is a 33 kDa member of the mitochondrial carrier protein family. UCP2 uncouples respiration from oxidative phosphorylation, principally in fat and skeletal muscle. Unlike UCP1, which generates heat in response to cold, UCP2 generates heat in response to dietary fluctuations. Human UCP2 is 309 amino acid (aa) in length. It contains six transmembrane domains (aa’s 11‑291) and is embedded in the inner mitochondrial membrane. Here, it dimerizes, forming a proton channel. There is one nucleotide binding site (aa 276‑298). Full‑length UCP2 shares 94%, 97% and 96% aa identity with porcine, canine and mouse UCP2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for UCP2 Antibody (AF4739R). (Showing 1 - 1 of 1 FAQ).

1. Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.
   • Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.

Bioinformatics

• Uniprot:
  • P55851()