UCP1 Antibody (536435) [Unconjugated]

Images

 
Western blot shows lysates of mouse brown adipose tissue and mouse adipose tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by ...read more
Western blot shows recombinant human UCP1, recombinant human UCP2, recombinant human UCP3, and recombinant human UCP4 (5 ng/lane). PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal ...read more
Simple Western lane view shows lysates of mouse adipose tissue and mouse brown adipose tissue, loaded at 0.5 mg/mL. A specific band was detected for UCP1 at approximately 37 kDa (as indicated) using 2.5 µg/mL of Mouse ...read more
UCP1 was detected in immersion fixed human mesenchymal stem cells undifferentiated (lower panel) or differentiated into adipocytes (upper panel) using Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) ...read more
3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF
Clone
536435
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

UCP1 Antibody (536435) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human UCP1
Met1-Thr307
Accession # P25874
Specificity
Detects human and mouse UCP1 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UCP1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 2.5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB6158 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UCP1 Antibody (536435) [Unconjugated]

  • SLC25A7
  • SLC25A7mitochondrial brown fat uncoupling protein 1
  • Solute carrier family 25 member 7
  • Thermogenin
  • UCP 1
  • UCP
  • UCP1
  • uncoupling protein 1 (mitochondrial, proton carrier)

Background

Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UCP1 Antibody (MAB6158)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(3)
IHC
(1)
IHC-Fr
(1)
Western Blot
(16)
All Applications
Filter By Species
Human
(10)
Mouse
(12)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using MAB6158 Applications Species
Y Takeda, P Dai Capsaicin directly promotes adipocyte browning in the chemical compound-induced brown adipocytes converted from human dermal fibroblasts Scientific Reports, 2022;12(1):6612. 2022 [PMID: 35459786] (Western Blot, Human) Western Blot Human
M Jumabay, L Zhang, J Yao, KI Boström Progenitor cells from brown adipose tissue undergo neurogenic differentiation Scientific Reports, 2022;12(1):5614. 2022 [PMID: 35379860] (ICC, Mouse) ICC Mouse
KM Choi, JH Kim, X Kong, M Isik, J Zhang, HW Lim, JC Yoon Defective brown adipose tissue thermogenesis and impaired glucose metabolism in mice lacking Letmd1 Cell Reports, 2021;37(11):110104. 2021 [PMID: 34910916] (Western Blot, Mouse) Western Blot Mouse
B B Tóth, Z Barta, ÁB Barta, L Fésüs Regulatory modules of human thermogenic adipocytes: functional genomics of large cohort and Meta-analysis derived marker-genes BMC Genomics, 2021;22(1):886. 2021 [PMID: 34895148] (Western Blot, Human) Western Blot Human
D Halbgebaue, J Roos, JB Funcke, H Neubauer, BS Hamilton, E Simon, EZ Amri, KM Debatin, M Wabitsch, P Fischer-Po, D Tews Latent TGFbeta-binding proteins regulate UCP1 expression and function via TGFbeta2 Molecular Metabolism, 2021;53(0):101336. 2021 [PMID: 34481123] (Western Blot, Human) Western Blot Human
J Sostre-Col, K Uehara, AE Garcia Whi, MJ Gavin, J Ishibashi, MJ Potthoff, P Seale, PM Titchenell Hepatic AKT orchestrates adipose tissue thermogenesis via FGF21-dependent and -independent mechanisms Cell Reports, 2021;35(7):109128. 2021 [PMID: 34010646] (Western Blot, Mouse) Western Blot Mouse
A Whitehead, FN Krause, A Moran, ADV MacCannell, JL Scragg, BD McNally, E Boateng, SA Murfitt, S Virtue, J Wright, J Garnham, GR Davies, J Dodgson, JE Schneider, AJ Murray, C Church, A Vidal-Puig, KK Witte, JL Griffin, LD Roberts Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis Nature Communications, 2021;12(1):1905. 2021 [PMID: 33772024] (ICC, Human) ICC Human
Y Takeda, T Yoshikawa, P Dai Transcriptome analysis reveals brown adipogenic reprogramming in chemical compound-induced brown adipocytes converted from human dermal fibroblasts Scientific Reports, 2021;11(1):5061. 2021 [PMID: 33658606] (Western Blot, Human) Western Blot Human
R Khatib Sha, J M Hoffmann, S Hedjazifar, L Bonnet, R K Baboota, S Heasman, C Church, I Elias, F Bosch, J Boucher, A Hammarsted, U Smith Adult mice are unresponsive to AAV8-Gremlin1 gene therapy targeting the liver PLoS ONE, 2021;16(2):e0247300. 2021 [PMID: 33606810] (Western Blot, Mouse) Western Blot Mouse
L Chen, J Zhang, Y Zou, F Wang, J Li, F Sun, X Luo, M Zhang, Y Guo, Q Yu, P Yang, Q Zhou, Z Chen, H Zhang, Q Gong, J Zhao, DL Eizirik, Z Zhou, F Xiong, S Zhang, CY Wang Kdm2a deficiency in macrophages enhances thermogenesis to protect mice against HFD-induced obesity by enhancing H3K36me2 at the Pparg locus Cell Death and Differentiation, 2021;0(0):. 2021 [PMID: 33462408] (IHC, Mouse) IHC Mouse
Show All 22 Publications.

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Bioinformatics

Gene Symbol UCP1
Uniprot