UCP1 Antibody (536435) [Unconjugated]


Western blot shows lysates of mouse brown adipose tissue and mouse adipose tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by ...read more
Western blot shows recombinant human UCP1, recombinant human UCP2, recombinant human UCP3, and recombinant human UCP4 (5 ng/lane). PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal ...read more
Simple Western lane view shows lysates of mouse adipose tissue and mouse brown adipose tissue, loaded at 0.5 mg/mL. A specific band was detected for UCP1 at approximately 37 kDa (as indicated) using 2.5 µg/mL of Mouse ...read more
3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more
UCP1 was detected in immersion fixed human mesenchymal stem cells undifferentiated (lower panel) or differentiated into adipocytes (upper panel) using Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF

Order Details

UCP1 Antibody (536435) [Unconjugated] Summary

E. coli-derived recombinant human UCP1
Accession # P25874
Detects human and mouse UCP1 in Western blots.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using
MAB6158 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UCP1 Antibody (536435) [Unconjugated]

  • SLC25A7
  • SLC25A7mitochondrial brown fat uncoupling protein 1
  • Solute carrier family 25 member 7
  • Thermogenin
  • UCP 1
  • UCP
  • UCP1
  • uncoupling protein 1 (mitochondrial, proton carrier)


Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UCP1 Antibody (MAB6158)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC-Fr, Western Blot.

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Flow Cytometry
Western Blot
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MAB6158 Applications Species
A Whitehead, FN Krause, A Moran, ADV MacCannell, JL Scragg, BD McNally, E Boateng, SA Murfitt, S Virtue, J Wright, J Garnham, GR Davies, J Dodgson, JE Schneider, AJ Murray, C Church, A Vidal-Puig, KK Witte, JL Griffin, LD Roberts Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis Nature Communications, 2021;12(1):1905. 2021 [PMID: 33772024] (ICC, Human) ICC Human
Y Takeda, T Yoshikawa, P Dai Transcriptome analysis reveals brown adipogenic reprogramming in chemical compound-induced brown adipocytes converted from human dermal fibroblasts Scientific Reports, 2021;11(1):5061. 2021 [PMID: 33658606] (Western Blot, Human) Western Blot Human
R Khatib Sha, J M Hoffmann, S Hedjazifar, L Bonnet, R K Baboota, S Heasman, C Church, I Elias, F Bosch, J Boucher, A Hammarsted, U Smith Adult mice are unresponsive to AAV8-Gremlin1 gene therapy targeting the liver PLoS ONE, 2021;16(2):e0247300. 2021 [PMID: 33606810] (Western Blot, Mouse) Western Blot Mouse
K Diallo, S Dussault, C Noll, AF Lopez, A Rivard, AC Carpentier, GE Lim 14-3-3? mediates an alternative, non-thermogenic mechanism in male mice to reduce heat loss and improve cold tolerance Mol Metab, 2020;41(0):101052. 2020 [PMID: 32668300] (ICC, Mouse) ICC Mouse
AR Angueira, SN Shapira, J Ishibashi, S Sampat, J Sostre-Col, MJ Emmett, PM Titchenell, MA Lazar, HW Lim, P Seale Early B Cell Factor Activity Controls Developmental and Adaptive Thermogenic Gene Programming in Adipocytes Cell Rep, 2020;30(9):2869-2878.e4. 2020 [PMID: 32130892] (Western Blot, Mouse) Western Blot Mouse
Y Takeda, P Dai A developed serum-free medium and an optimized chemical cocktail for direct conversion of human dermal fibroblasts into brown adipocytes Sci Rep, 2020;10(1):3775. 2020 [PMID: 32111895] (Western Blot, Human) Western Blot Human
JM Hoffmann, JR Grünberg, A Hammarsted, T Kroon, TU Greiner, S Maurer, I Elias, V Palsdottir, F Bosch, J Boucher, S Hedjazifar, U Smith BMP4 gene therapy enhances insulin sensitivity but not adipose tissue browning in obese mice Mol Metab, 2020;32(0):15-26. 2020 [PMID: 32029225] (Western Blot, Mouse) Western Blot Mouse
Á Klusóczki, Z Veréb, A Vámos, P Fischer-Po, M Wabitsch, Z Bacso, L Fésüs, E Kristóf Differentiating SGBS adipocytes respond to PPAR? stimulation, irisin and BMP7 by functional browning and beige characteristics Sci Rep, 2019;9(1):5823. 2019 [PMID: 30967578] (Western Blot, Human) Western Blot Human
MJ Harms, Q Li, S Lee, C Zhang, B Kull, S Hallen, A Thorell, I Alexanders, CE Hagberg, XR Peng, A Mardinoglu, KL Spalding, J Boucher Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes Cell Rep, 2019;27(1):213-225.e5. 2019 [PMID: 30943403] (Western Blot, Human) Western Blot Human
S Su, AR Guntur, DC Nguyen, SS Fakory, CC Doucette, C Leech, H Lotana, M Kelley, J Kohli, J Martino, S Sims-Lucas, L Liaw, C Vary, CJ Rosen, AC Brown A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome Cell Rep, 2018;25(11):3215-3228.e9. 2018 [PMID: 30540952] (Western Blot, Mouse) Western Blot Mouse
Show All 15 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 IC6158U
Alexa Fluor 405 IC6158V
Alexa Fluor 488 IC6158G
Alexa Fluor 532 FAB6158X
Alexa Fluor 594 IC6158T
Alexa Fluor 647 IC6158R
Alexa Fluor 700 IC6158N
Alexa Fluor 750 IC6158S
Allophycocyanin FAB6158A
Biotin FAB6158B
DyLight 350 FAB6158D
DyLight 405 FAB6158E
DyLight 488 FAB6158K
DyLight 550 FAB6158L
DyLight 594 FAB6158M
DyLight 650 FAB6158W
DyLight 680 FAB6158Y
DyLight 755 FAB6158Z
Janelia Fluor 549 FAB6158I
Janelia Fluor 646 FAB6158J
PerCP FAB6158C
Phycoerythrin IC6158P
Phycoerythrin IC6158P

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Gene Symbol UCP1