UCP2 Antibody [Alexa Fluor® 594]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

UCP2 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human UCP2
  Met1-Phe309
  Accession # P55851
• Specificity: Detects human and mouse UCP2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) UCP1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Mitochondrial Uncoupling Protein 2 (UCP2) is a 33 kDa member of the mitochondrial carrier protein family. UCP2 uncouples respiration from oxidative phosphorylation, principally in fat and skeletal muscle. Unlike UCP1, which generates heat in response to cold, UCP2 generates heat in response to dietary fluctuations. Human UCP2 is 309 amino acid (aa) in length. It contains six transmembrane domains (aa’s 11‑291) and is embedded in the inner mitochondrial membrane. Here, it dimerizes, forming a proton channel. There is one nucleotide binding site (aa 276‑298). Full‑length UCP2 shares 94%, 97% and 96% aa identity with porcine, canine and mouse UCP2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for UCP2 Antibody (AF4739T). (Showing 1 - 1 of 1 FAQ).

1. Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.
   • Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.