UCH-L1/PGP9.5 Antibody


Western Blot: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Blot of whole bovine brain extract stained with RPCA-UCHL1 showing a strong and clean band at about 24kDa.
Immunocytochemistry/ Immunofluorescence: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Shows rat mixed neuron/glial cultures stained with Rabbit anti-UCHL1 antibody (green). Blue is a DNA stain. Note that the UCHL1 stains ...read more
Simple Western: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Simple Western lane view shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions ...read more

Product Details

Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

UCH-L1/PGP9.5 Antibody Summary

Recombinant full length human UCHL1 purified from E. coli. [UniProt# P09936]
pan-Neuronal Marker
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
This UCHL1 antibody is useful for Immunocytochemistry/Immunofluorescence, ABC or other enzyme linked immunocytochemical procedures and Western blotting. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 22527779)In Simple Western only 10-15 uL of the recommended dilution is used per data point.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
24 kDa
Read Publication using
NB110-58874 in the following applications:

Reactivity Notes

Human, rat, mouse, cow and pig.

Alternate Names for UCH-L1/PGP9.5 Antibody

  • EC
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1


UCH-L1 (ubiquitin carboxyl-terminal hydrolase isozyme L1) was the first discovered de-ubiquitinating enzyme which implicates in processing of ubiquitin precursors and also of ubiquitinated proteins. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at C-terminal glycine of ubiquitin. It also binds to free monoubiquitin and prevents its degradation in lysosomes. Localized in cytoplasm and ER membrane as lipid-anchor, UCH-L1 expression is restricted to brain, peripheral nerves, endocrine tissues and gonads of both sexes etc. UCH-L1 deletion in mice leads to fatal neurodegenerative disorder known as gracile axonal dystrophy and it is down-regulated in brains from Parkinson as well as Alzheimer disease patients. Expression outside of neuro-endocrine tissues is found in various cancers including B-cell lymphoma, multiple myeloma, and lung cancer. In transgenic mouse model, UCH-L1 has been demonstrated as an oncogene that causes malignancies by boosting AKT signalling. Furthermore, UCH-L1 has been shown to interfere with ubiquitination of RAPTOR which is catalyzed by DDB1-Cul4 E3 ligase complex, leading to loss of mTORC1 integrity accompanied by a concurrent increase in mTORC2, likely due to increased availability of free mTOR.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, S-ELISA
Species: Hu, Mu, Rt, Rb, Xp, Ze
Applications: WB, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr
Species: Hu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Flow, IHC
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Dr, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF
Species: Mu
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo
Species: Hu
Applications: Func, PAGE
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Ch, Mk
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ch
Applications: WB, Simple Western, ICC/IF, IP, In vitro

Publications for UCH-L1/PGP9.5 Antibody (NB110-58874)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publication 1 - 1 of 1.
Publication using NB110-58874 Applications Species
Marmotti A, Cravino M, Germano M et al. Peroneal tendoscopy. Curr Rev Musculoskelet Med. 2012 Jun [PMID:22527779] (IHC-P, Human) IHC-P Human

Reviews for UCH-L1/PGP9.5 Antibody (NB110-58874) (0)

There are no reviews for UCH-L1/PGP9.5 Antibody (NB110-58874). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UCH-L1/PGP9.5 Antibody (NB110-58874) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Additional UCH-L1/PGP9.5 Antibody Products

Related Products by Gene

Bioinformatics Tool for UCH-L1/PGP9.5 Antibody (NB110-58874)

Discover related pathways, diseases and genes to UCH-L1/PGP9.5 Antibody (NB110-58874). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for UCH-L1/PGP9.5 Antibody (NB110-58874)

Discover more about diseases related to UCH-L1/PGP9.5 Antibody (NB110-58874).

Pathways for UCH-L1/PGP9.5 Antibody (NB110-58874)

View related products by pathway.

PTMs for UCH-L1/PGP9.5 Antibody (NB110-58874)

Learn more about PTMs related to UCH-L1/PGP9.5 Antibody (NB110-58874).

Research Areas for UCH-L1/PGP9.5 Antibody (NB110-58874)

Find related products by research area.

Blogs on UCH-L1/PGP9.5

There are no specific blogs for UCH-L1/PGP9.5, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol UCHL1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB110-58874 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought