UCH-L1/PGP9.5 Antibody

Images

 
Western Blot: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Blot of whole bovine brain extract stained with RPCA-UCHL1 showing a strong and clean band at about 24kDa.
Immunocytochemistry/ Immunofluorescence: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Shows rat mixed neuron/glial cultures stained with Rabbit anti-UCHL1 antibody (green). Blue is a DNA stain. Note that the UCHL1 stains ...read more
Simple Western: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Simple Western lane view shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

UCH-L1/PGP9.5 Antibody Summary

Immunogen
Recombinant full length human UCHL1 purified from E. coli. [UniProt# P09936]
Marker
pan-Neuronal Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UCHL1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:2000
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry-Paraffin
Application Notes
This UCHL1 antibody is useful for Immunocytochemistry/Immunofluorescence, ABC or other enzyme linked immunocytochemical procedures and Western blotting. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 22527779)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
UCH-L1/PGP9.5 Lysate (NBL1-17575)
Publications
Read Publication using
NB110-58874 in the following applications:

Reactivity Notes

Human, rat, mouse, cow and pig.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
10mM Sodium Azide
Purity
Unpurified

Alternate Names for UCH-L1/PGP9.5 Antibody

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

UCH-L1 (ubiquitin carboxyl-terminal hydrolase isozyme L1) was the first discovered de-ubiquitinating enzyme which implicates in processing of ubiquitin precursors and also of ubiquitinated proteins. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at C-terminal glycine of ubiquitin. It also binds to free monoubiquitin and prevents its degradation in lysosomes. Localized in cytoplasm and ER membrane as lipid-anchor, UCH-L1 expression is restricted to brain, peripheral nerves, endocrine tissues and gonads of both sexes etc. UCH-L1 deletion in mice leads to fatal neurodegenerative disorder known as gracile axonal dystrophy and it is down-regulated in brains from Parkinson as well as Alzheimer disease patients. Expression outside of neuro-endocrine tissues is found in various cancers including B-cell lymphoma, multiple myeloma, and lung cancer. In transgenic mouse model, UCH-L1 has been demonstrated as an oncogene that causes malignancies by boosting AKT signalling. Furthermore, UCH-L1 has been shown to interfere with ubiquitination of RAPTOR which is catalyzed by DDB1-Cul4 E3 ligase complex, leading to loss of mTORC1 integrity accompanied by a concurrent increase in mTORC2, likely due to increased availability of free mTOR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UCH-L1/PGP9.5 Antibody (NB110-58874)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Filter By Species
Human
(1)
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Showing Publication 1 - 1 of 1.
Publication using NB110-58874 Applications Species
Marmotti A, Cravino M, Germano M et al. Peroneal tendoscopy. Curr Rev Musculoskelet Med. 2012 Jun [PMID: 22527779] (IHC-P, Human) IHC-P Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UCHL1
Entrez
Uniprot