UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA
Clone
31A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free Summary

Immunogen
Native UchL1 (PGP9.5) protein from brain was used as immunogen to generate the antibody (Day & Thompson, 1986).
Localization
Cytoplasmic, Endoplasmic Reticulum membrane
Marker
pan-Neuronal Marker
Specificity
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UCHL1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
Application Notes
Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Specimens which have not been fixed in acetic acid/alcohol require pre-treatment with citrate buffer: for example, unmask of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min. This antibody has been reported to work in IHC-Frozen as reported in the literature (Bottner et al. Drew et al.) and (Sugimoto et al. J Hyperten)
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UCHL1
Entrez