Tyrosine Hydroxylase [p Ser40] Antibody

Images

 
Independent Antibodies: Immunohistochemistry: Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and phospho-specific tyrosine ...read more
Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. ...read more

Product Details

Summary
Reactivity Mu, Rt, MaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.1 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

Tyrosine Hydroxylase [p Ser40] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 conjugated to KLH
Modification
p Ser40
Marker
Neuronal Marker
Specificity
Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunofluorescence 1:10-1:500
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB300-173 in the following applications:

Publications
Read Publications using
NB300-173 in the following applications:

Reactivity Notes

Reactivity assumed based on sequence identity to a wide variety of mammalian and non-mammalian species.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
0.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tyrosine Hydroxylase [p Ser40] Antibody

  • DYT14
  • DYT5b
  • EC 1.14.16
  • EC 1.14.16.2
  • TH
  • TYH dystonia 14
  • TYH
  • Tyrosine 3-hydroxylase
  • tyrosine 3-monooxygenase
  • Tyrosine Hydroxylase

Background

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore, the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. TH has a large molecular diversity, resulting from differential splicing of its mRNA, which is tissue specific and might result in long term changes in activity of the enzyme and its availability of neurotransmitter at various synapses. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency, as low dopamine levels are a consistent neurochemical abnormality.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-173
Species: Mu, Rt, Ma
Applications: WB, ICC/IF, IHC, IHC-Fr, ICC/IF

Publications for Tyrosine Hydroxylase Antibody (NB300-173)(7)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-Fr
(2)
WB
(2)
All Applications
Filter By Species
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB300-173 Applications Species
Acosta G, Race N, Herr S et al. Acrolein-mediated alpha-synuclein pathology involvement in the early post-injury pathogenesis of mild blast-induced Parkinsonian neurodegeneration Mol. Cell. Neurosci. 2019 Jun 12 [PMID: 31201929] (WB, Rat) WB Rat
Acosta GHG. Susceptibility of parkinson's disease following mild blast traumatic brain injury Thesis. 2014 Aug 01 (WB, Rat) WB Rat
Gassmann M, Pfistner C, Doan VD et al. Impaired ventilatory acclimatization to hypoxia in female mice overexpressing erythropoietin: unexpected deleterious effect of estradiol in carotid bodies. Am J Physiol Regul Integr Comp Physiol 2010 Dec [PMID: 20861276] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Soliz J, Joseph V, Soulage C et al. Erythropoietin regulates hypoxic ventilation in mice by interacting with brainstem and carotid bodies J Physiol. 2005 Oct 15 [PMID: 16051624] (IHC, Mouse) IHC Mouse
Witkovsky P, Gabriel R, Haycock JW et al. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat. 2000 Jun [PMID: 10936746] (IHC-Fr) IHC-Fr
Salvatore, MF et al. Stoichiometry of tyrosine hydroxylase phosphorylation in the nigrostriatal mesolimbic systems in vivo: effects of acute haloperidol related compounds. J Neurochem, 75: 225-232. 2000 [PMID: 10854265]
Lew, JY et al. Increased site-specific phosphorylation of tyrosine hydroxylase accompanies stimulation of enzymatic activity induced by cessation of dopamine neuronal activity. Mol Pharm, 55: 202-209. 1998 [PMID: 9927609]

Review for Tyrosine Hydroxylase Antibody (NB300-173) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB300-173:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-Fr Mouse 03/23/2010
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse brain
SpeciesMouse
Lotajo1009

Blocking

Blocking DetailsBlocking Buffer: goat serum, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: tbs/ 0.1% triton x-100/ 1% goat serum, Incubation Time: overnight, Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: biotinylated anti rabbit

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tyrosine Hydroxylase Antibody (NB300-173) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Tyrosine Hydroxylase Antibody (NB300-173)

Discover related pathways, diseases and genes to Tyrosine Hydroxylase Antibody (NB300-173). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Tyrosine Hydroxylase Antibody (NB300-173)

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Blogs on Tyrosine Hydroxylase.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Tyrosine hydroxylase - a marker for dopaminergic neurons in the central nervous system
Tyrosine hydroxylase is a member of the aromatic amino acid hydroxylase (AAAH) family.  It is expressed throughout the central nervous system (CNS) and catalyzes the conversion of tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), which can be, thr...  Read full blog post.

Tyrosine Hydroxylase - rate-limiting enzyme in catecholamine synthesis
Catecholamines are tyrosine-derived hormones that are produced in the adrenal gland. They include epinephrine, norepinephrine, and dopamine and are used as neurotransmitters by the central and peripheral nervous system. The rate limiting enzyme in...  Read full blog post.

A Big Guy for the Catecholamine Synthesis - Tyrosine hydroxylase (TH)
In the synthesis pathway for the catecholamines - dopamine, epinephrine, and norepinephrine, tyrosine hydroxylase is the rate-limiting enzyme. Through alternative mRNA splicing, a wide molecular diversity of TH isoforms are generated that are tissue-s...  Read full blog post.

Tyrosine Hydroxylase Deficiencies and Neurodegeneration
Tyrosine hydroxylase is the rate-limiting enzyme in the synthesis pathway of the catecholamines dopamine, epinephrine, and norepinephrine. Alternative mRNA splicing generates a wide molecular diversity of TH isoforms that are tissue specific and produ...  Read full blog post.

Tyrosine Hydroxylase Deficiency and Brain Disorders
 Tyrosine hydroxylase catalyzes the rate-limiting step in the biosynthesis of the catecholamines dopamine, norepinephrine, and epinephrine. A hallmark of Parkinson's disease is the loss of dopaminergic neurons in the substantia nigra. Mutations in cas...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
03/23/2010
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol TH
Entrez
Uniprot