TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free

Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Analysis using the Biotin conjugate of NBP2-00409. Staining of TWEAK R in murine 603B cell lines using anti-TWEAK R antibody. Image from verified customer review.

Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a K Isotype Control Purified (open histogram) or 0.5 ug Anti-Human/Mouse CD266 (TWEAK Receptor) Purified (filled histogram) followed byAnti-Mouse IgG FITC. Total viable cells were used for analysis.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC
• Clone: ITEM-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free Summary

• Description: Novus Biologicals Mouse TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free (NBP2-00409) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody is TWEAKR.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF12A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This has been tested by flow cytometric analysis on human or mouse Fn14 transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Use in WB and IHC-F was reported in the scientific literature (PMID: 19473660).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free

• CD266 antigen
• CD266
• FGF-inducible 14
• Fibroblast growth factor-inducible immediate-early response protein 14
• Fn14
• FN14tumor necrosis factor receptor superfamily member 12A
• TNFRSF12
• TNFRSF12A
• tumor necrosis factor receptor superfamily, member 12A
• TWEAK R
• TWEAKR
• tweak-receptor
• type I transmembrane protein Fn14

Background

The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF12A