TWEAK R/TNFRSF12 Antibody (ITEM-4)


Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Analysis using the Biotin conjugate of NBP2-00409. Staining of TWEAK R in murine 603B cell lines using anti-TWEAK R antibody. Image from verified more
Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a K Isotype Control Purified (open histogram) more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr
0.5 mg/ml

TWEAK R/TNFRSF12 Antibody (ITEM-4) Summary

The immunogen for this antibody is TWEAKR.
IgG2b Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


Application Notes
This has been tested by flow cytometric analysis on human or mouse Fn14 transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Use in WB and IHC-F was reported in the scientific literature (PMID: 19473660).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for TWEAK R/TNFRSF12 Antibody (ITEM-4)

  • CD266 antigen
  • CD266
  • FGF-inducible 14
  • Fibroblast growth factor-inducible immediate-early response protein 14
  • Fn14
  • FN14tumor necrosis factor receptor superfamily member 12A
  • TNFRSF12
  • tumor necrosis factor receptor superfamily, member 12A
  • tweak-receptor
  • type I transmembrane protein Fn14


The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP2-00409AF405
Alexa Fluor (R) 488 Labeled NBP2-00409AF488
Alexa Fluor (R) 647 Labeled NBP2-00409AF647
Alexa Fluor (R) 700 Labeled NBP2-00409AF700
Allophycocyanin Labeled NBP2-00409APC
Biotin Labeled NBP1-43483
DyLight 350 Labeled NBP2-00409UV
DyLight 405 Labeled NBP2-00409V
DyLight 405LS Labeled NBP2-00409V3
DyLight 488 Labeled NBP2-00409G
DyLight 680 Labeled NBP2-00409FR
DyLight 755 Labeled NBP2-00409IR
PE Labeled NBP2-00409PE
PerCP Labeled NBP2-00409PCP

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Contact Information

Product PDFs


Gene Symbol TNFRSF12A

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