Recombinant Human TWEAK/TNFSF12 Protein


1 μg/lane of Recombinant Human TWEAK/TNFSF12 was resolved withSDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 2‑8 ng/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TWEAK/TNFSF12 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.200-4.00 ng/mL.
E. coli-derived human TWEAK/TNFSF12 protein
Arg93-His249, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
18.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
1090-TW in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TWEAK/TNFSF12 Protein

  • APO3 ligand
  • APO3LMGC20669
  • DR3LGTWEAKAPO3/DR3 ligand
  • MGC129581
  • TNF-related weak inducer of apoptosis
  • TNFSF12
  • tumor necrosis factor (ligand) superfamily, member 12
  • tumor necrosis factor ligand superfamily member 12


TWEAK is a type II transmembrane protein belonging to the TNF superfamily (1). It contains a short cytoplasmic domain (aa 1‑18), the transmembrane domain
(aa 19-42) and an extracellular domain (aa 43-249). The extracellular domains of human and murine TWEAK share 89% aa sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage after Arg 93 suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells. At least two receptors that bind TWEAK have been identified (2-4). Death Receptor 3 (DR3), also known as TNFRSF12, Apo-3, LARD, WSL-1 or TRAMP, is a TNF receptor superfamily member that is expressed predominantly in tissues with high lymphocyte content (2). It has been suggested that induction of cell death by TWEAK-DR3 interaction involves the activation of NF-kappa B. In cells that lack DR3, alternate pathways of TWEAK-induced cell death mediated by receptors distinct from DR3 have been suggested (5, 6). TWEAK receptor (TWEAKR, alternatively known as FN14), is a novel TNF receptor superfamily member that also binds TWEAK (3, 4). It is a mitogen-inducible gene that is expressed in fibroblasts, hepetocellular carcinomas and endothelial cells. TWEAK-TWEAKR interaction has been shown to play a role in endothelial cell growth and migration. This effect of TWEAK is not mediated by an up-regulation of VEGF (7).

  1. Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
  2. Marsters, S. et al. (1998) Current Biol. 8:525.
  3. Wiley, S. R. et al. (2001) Immunity, 15:837.
  4. Feng, S.-L.Y. et al. (2000) Am J. Path. 156:1253.
  5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
  6. Schneider, P. et al. (1999) Eur. J. Immunol., 29:1785.
  7. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.

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Publications for TWEAK/TNFSF12 (1090-TW)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Bioassay, Surface Plasmon Resonance, Surface Plasmon Resonance (SPR.

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Surface Plasmon Resonance
Surface Plasmon Resonance (SPR
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 1090-TW Applications Species
M Badia-Vill, S Defaus, R Foj, D Andreu, B Oliva, A Sierra, N Fernandez- Evaluation of Computationally Designed Peptides against TWEAK, a Cytokine of the Tumour Necrosis Factor Ligand Family International Journal of Molecular Sciences, 2021;22(3):. 2021 [PMID: 33494438] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
X Wang, D Cheng, G Hu, L Liang, F Tan, T Xiao, S Xiao, Y Xia Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis Mediators Inflamm., 2019;2019(0):2945083. 2019 [PMID: 31885495] (Bioassay, Human) Bioassay Human
SJ Lee, J Kim, J Ko, EJ Lee, HJ Koh, JS Yoon Tumor necrosis factor-like weak inducer of apoptosis induces inflammation in Graves&#039; orbital fibroblasts PLoS ONE, 2018;13(12):e0209583. 2018 [PMID: 30576385] (Bioassay, Human) Bioassay Human
AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018;0(0):1-43. 2018 [PMID: 29436901] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
L Xue, L Liu, J Huang, J Wen, R Yang, L Bo, M Tang, Y Zhang, Z Liu Tumor Necrosis Factor-Like Weak Inducer of Apoptosis Activates Type I Interferon Signals in Lupus Nephritis Biomed Res Int, 2017;2017(0):4927376. 2017 [PMID: 29333443] (Bioassay, Human) Bioassay Human
HD Brightbill, E Suto, N Blaquiere, N Ramamoorth, S Sujatha-Bh, EB Gogol, GM Castanedo, BT Jackson, YC Kwon, S Haller, J Lesch, K Bents, C Everett, PB Kohli, S Linge, L Christian, K Barrett, A Jaochico, LM Berezhkovs, PW Fan, Z Modrusan, K Veliz, MJ Townsend, J DeVoss, AR Johnson, R Godemann, WP Lee, CD Austin, BS McKenzie, JA Hackney, JJ Crawford, ST Staben, MH Alaoui Ism, LC Wu, N Ghilardi NF-?B inducing kinase is a therapeutic target for systemic lupus erythematosus Nat Commun, 2018;9(1):179. 2018 [PMID: 29330524] (Bioassay, Human) Bioassay Human
D Sidler, P Wu, R Herro, M Claus, D Wolf, Y Kawakami, T Kawakami, L Burkly, M Croft TWEAK mediates inflammation in experimental atopic dermatitis and psoriasis Nat Commun, 2017;8(0):15395. 2017 [PMID: 28530223] (Bioassay, Human) Bioassay Human
A Wang, F Zhang, H Xu, M Xu, Y Cao, C Wang, Y Xu, M Su, M Zhang, Y Zhuge TWEAK/Fn14 promotes pro-inflammatory cytokine secretion in hepatic stellate cells via NF-?B/STAT3 pathways Mol. Immunol., 2017;87(0):67-75. 2017 [PMID: 28411440] (Bioassay, Human) Bioassay Human
N Yamaguchi, Y Nakayama, N Yamaguchi Down-regulation of Forkhead Box Protein A1 (FOXA1) Leads to Cancer-stem Cell-like Properties in Tamoxifen-resistant Breast Cancer Cells through Induction of Interleukin-6 J. Biol. Chem, 2017;0(0):. 2017 [PMID: 28270510] (Bioassay, Human) Bioassay Human
ME Guicciardi, A Krishnan, SF Bronk, P Hirsova, TS Griffith, GJ Gores Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice Cell Death Dis, 2017;8(1):e2535. 2017 [PMID: 28055006] (Bioassay, Human) Bioassay Human
Show All 14 Publications.

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Gene Symbol TNFSF12