1199-TW) inhibits Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human TWEAK/TNFSF12 (20 ng/mL) activity elicited by Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1199). The ND50 is typically 0.3-1.2 µg/mL." alt="Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199-TW) inhibits Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human TWEAK/TNFSF12 (20 ng/mL) activity elicited by Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1199). The ND50 is typically 0.3-1.2 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1199-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35085-1mg | |||
| Carrier-Free | NBP2-35085-100ug | |||
| Carrier-Free | NBP2-35085-500ug | |||
CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="TWEAK R/TNFRSF12 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1610) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1610-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1199-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85770-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34499-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB16101-SP | |||
| Carrier Free | FAB16101V-100UG | |||
| Carrier Free | FAB16101U-100UG | |||
| Carrier Free | FAB16101T-100UG | |||
| Carrier Free | FAB16101S-100UG | |||
| Carrier Free | FAB16101R-100UG | |||
| Carrier Free | FAB16101N-100UG | |||
| Carrier Free | FAB16101G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1610-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-00409-25ug | |||
![Expression of TWEAK and Fn14 in renal biopsies from IgAN patients was examine. Immunohistochemistry for both TWEAK (A, B) and Fn14 (D, E) were detected in glomerular crescents (the areas delineated by the squares). In the controls [renal biopsies from patients with minimal change disease (C, F)], there was very slight staining for TWEAK and Fn14 in glomeruli, while intense staining was observed in renal tubular cells. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25885587), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af1199_human-tweak-r-tnfrsf12-affinity-purified-pab-8220249505954.jpg)
![Expression of TWEAK and Fn14 in renal biopsies from IgAN patients was examine. Immunohistochemistry for both TWEAK (A, B) and Fn14 (D, E) were detected in glomerular crescents (the areas delineated by the squares). In the controls [renal biopsies from patients with minimal change disease (C, F)], there was very slight staining for TWEAK and Fn14 in glomeruli, while intense staining was observed in renal tubular cells. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25885587), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af1199_human-tweak-r-tnfrsf12-affinity-purified-pab-8220249504645.jpg)
![Expression of TWEAK and Fn14 in renal biopsies from IgAN patients was examine. Immunohistochemistry for both TWEAK (A, B) and Fn14 (D, E) were detected in glomerular crescents (the areas delineated by the squares). In the controls [renal biopsies from patients with minimal change disease (C, F)], there was very slight staining for TWEAK and Fn14 in glomeruli, while intense staining was observed in renal tubular cells. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25885587), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af1199_human-tweak-r-tnfrsf12-affinity-purified-pab-8220249505937.jpg)
![Expression of TWEAK and Fn14 in renal biopsies from IgAN patients was examine. Immunohistochemistry for both TWEAK (A, B) and Fn14 (D, E) were detected in glomerular crescents (the areas delineated by the squares). In the controls [renal biopsies from patients with minimal change disease (C, F)], there was very slight staining for TWEAK and Fn14 in glomeruli, while intense staining was observed in renal tubular cells. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25885587), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af1199_human-tweak-r-tnfrsf12-affinity-purified-pab-44202415425083.jpg)
![SDS-Page: Recombinant Human TWEAK R/TNFRSF12 Protein [NBP2-35085]](http://images.novusbio.com/fullsize/Recombinant-Human-TWEAK-R-TNFRSF12-Protein-SDS-Page-NBP2-35085-img0001.jpg "SDS-Page: Recombinant Human TWEAK R/TNFRSF12 Protein [NBP2-35085]")
1610-TW) was serially diluted and captured by Goat Anti-Mouse TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1610) coated on a Clear Polystyrene Microplate (Catalog # 
1610-TW) was serially diluted and captured by Goat Anti-Mouse TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # ![Western Blot: TWEAK R/TNFRSF12 Antibody [NB100-56636] - Analysis of TweakR in A) human liver lysate , B) mouse liver tissue lysate , and C) rat liver lysate using this antibody at 1:500.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-Western-Blot-NB100-56636-img0005.jpg "Western Blot: TWEAK R/TNFRSF12 Antibody [NB100-56636] - Analysis of TweakR in A) human liver lysate , B) mouse liver tissue lysate , and C) rat liver lysate using this antibody at 1:500.")
![Western Blot: TWEAK R/TNFRSF12 Antibody [NB100-56636] - Analysis of TweakR in 293 cells. Untransfected (lane 1) or transfectd with human TweakR (lane 2). Photograph courtesy of Dr. Taly Spivakk, The MD Anderson Cancer Center, Houston, TX.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-Western-Blot-NB100-56636-img0004.jpg)
![Immunohistochemistry-Paraffin: TWEAK R/TNFRSF12 Antibody [NBP1-85770] - Staining of human kidney shows moderate membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-Immunohistochemistry-Paraffin-NBP1-85770-img0005.jpg "Immunohistochemistry-Paraffin: TWEAK R/TNFRSF12 Antibody [NBP1-85770] - Staining of human kidney shows moderate membranous positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: TWEAK R/TNFRSF12 Antibody [NBP1-85770] - Staining of human stomach shows strong membranous positivity in glanular cells.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-Immunohistochemistry-Paraffin-NBP1-85770-img0006.jpg)
![Western Blot: TWEAK R/TNFRSF12 Antibody [NBP2-34499] - Analysis of Fn14 in HepG2 cells with Fn14 antibody at (A) 2 and (B) 4 ug/mL.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-Western-Blot-NBP2-34499-img0006.jpg "Western Blot: TWEAK R/TNFRSF12 Antibody [NBP2-34499] - Analysis of Fn14 in HepG2 cells with Fn14 antibody at (A) 2 and (B) 4 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: TWEAK R/TNFRSF12 Antibody - BSA Free [NBP2-34499] - Immunofluorescence of TWEAK R/TNFRSF12 in Human Liver cells with TWEAK R/TNFRSF12 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-34499_rabbit-polyclonal-tweak-r-tnfrsf12-antibody-2711202419201527.jpg)
![Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Analysis using the Biotin conjugate of NBP2-00409. Staining of TWEAK R in murine 603B cell lines using anti-TWEAK R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-ITEM-4-Flow-Cytometry-NBP2-00409-img0003.jpg "Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Analysis using the Biotin conjugate of NBP2-00409. Staining of TWEAK R in murine 603B cell lines using anti-TWEAK R antibody. Image from verified customer review.")
![Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a K Isotype Control Purified (open histogram) or 0.5 ug Anti-Human/Mouse CD266 (TWEAK Receptor) Purified (filled histogram) followed byAnti-Mouse IgG FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-ITEM-4-Flow-Cytometry-NBP2-00409-img0001.jpg)
