Tubulin [ac Lys40] Antibody (6-11B-1)

Images

 
Western Blot: Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - WB validation of monoclonal anti-Acetylated Tubulin antibody clone: 6-11B-1 at 1:2000 dilution with goat anti-Mouse IgG-Peroxidase secondary antibody - ...read more
Immunocytochemistry/ Immunofluorescence: Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - HeLa cells were fixed with 4% paraformaldehyde followed permeabilization with 0.5% Triton X-100. Fixed cells were stained with ...read more
Immunohistochemistry: Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - Nerve pathways in the embryonic Zebrafish brain and spinal cord revealed by Monoclonal Anti-Acetylated Tubulin (cat#NB600-567). Immunoperoxidase ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Am, Bv, Ca, Ch, Ha, In, Pa, Pl, Pm, PrSpecies Glossary
Applications WB, DB, ELISA, EM, ICC/IF, IHC, RIA
Clone
6-11B-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Tubulin [ac Lys40] Antibody (6-11B-1) Summary

Immunogen
Acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin)
Modification
ac Lys40
Localization
Centrosomal.
Marker
Microtubule Marker
Specificity
The antibody recognizes an epitope located on the alpha 3 isoform of Chlamydomonas axonemal alpha-tubulin, within four residues of Lys-40 when this amino acid is acetylated
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TUBA1A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Dot Blot 1:100-1:2000
  • Electron Microscopy 1:10-1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:10-1:500
  • Radioimmunoassay 1:100-1:2000
Publications
Read Publication using
NB600-567 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Tubulin [ac Lys40] Antibody (6-11B-1)

  • Alpha-tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

The major building block of microtubules is tubulin, an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta, that assemble into microtubules. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta tubulins consist of various isotypes. In addition, both undergo post-translational modifications, including acetylation, phosphorylation detyrosination, polyglutamylation, and polyglycylation. Polyglutamylation of tubulin consists of the addition of a lateral chain of glutamyl units on the C-terminal region of tubulin polypeptides. This modification was shown to regulate the interaction between tau, one of the major neuronal microtubule associated proteins, and tubulin. Furthermore, it was shown that injection of monoclonal antibodies specific to the polyglutamylated form of tubulin into HeLa cells caused the disappearance of centrioles, an organelle known to have the most stable microtubules. The detection, localization, and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport, and cytoskeletal functions. Antibodies reacting specifically with the modified forms of alpha and beta tubulin isotypes serve as essential tools in the detection and study of the functional significance of these molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-567
Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Ch, Ha, In, Pa, Pl, Pm, Pr
Applications: WB, DB, ELISA, EM, ICC/IF, IHC, RIA

Publications for Tubulin Antibody (NB600-567)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tubulin Antibody (NB600-567). (Showing 1 - 2 of 2 FAQ).

  1. I am interested in buying a Tubulin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Tubulin Antibody (YL1/2) catalog # NB600-506. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.
  2. The data sheet for Tubulin [ac Lys40] antibody (6-11B-1) (catalog number for Novus Biologicals: NB600-567) indicates that this antibody works in ELISA and I would like to request either the protocol or the literature reference for it at your earliest convenience. Do you also have the information on the capture antibody that you used and the input quantity available?
    • Here is a link to our ELISA protocol. NB600-567 was tested and validated for use in an indirect ELISA, and as such was used as the detection antibody. For the testing of this antibody, the ELISA plate wells were coated with the immunogen, acetylated a-tubulin from the outer arm of sea urchin sperm axonemes, and no capture antibody was used. Unfortunately I don't have any information regarding the input quantity, but please let me know if you have additional questions.

Secondary Antibodies

 

Isotype Controls

Additional Tubulin Products

Tubulin NB600-567

Bioinformatics Tool for Tubulin Antibody (NB600-567)

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Diseases for Tubulin Antibody (NB600-567)

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Blogs on Tubulin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

The GAPDH Antibody in Western blot Assays
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Who Needs Antibodies for Tubulin Modifications?
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Bioinformatics

Gene Symbol TUBA1A
Entrez