TRPC5 Antibody (N67/15)

Western Blot: TRPC5 Antibody (N67/15) [NBP2-12919] - Western Blot analysis of Mouse brain showing detection of 110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (NBP2-12919). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (NBP2-12919) at 1:1000 for Overnight at 4C. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:7000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 110 kDa.

Immunocytochemistry/Immunofluorescence: TRPC5 Antibody (N67/15) [NBP2-12919] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (NBP2-12919). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (NBP2-12919) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC5 Antibody (D) Composite.

Immunohistochemistry: TRPC5 Antibody (N67/15) [NBP2-12919] - Immunohistochemistry analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (NBP2-12919). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (NBP2-12919) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Nuclear staining. Magnification: 10X.

Western Blot: TRPC5 Antibody (N67/15) [NBP2-12919] - Rat Brain Membrane showing detection of ~110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-15. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody ) at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa. Other Band(s): 160kDa, 60kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, MA
• Clone: N67/15
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TRPC5 Antibody (N67/15) Summary

• Immunogen: Synthetic peptide amino acids 827-845 of human TrpC5 (also known as short transient receptor potential channel 5, and Htrp5)
• Localization: Membrane
• Specificity: Detects approx 110kDa.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRPC5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation
  • Microarray
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of TrpC5 Antibody was sufficient for detection of TrpC5 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for TRPC5 Antibody (N67/15)

• hTRP5
• hTRP-5
• short transient receptor potential channel 5
• transient receptor potential cation channel, subfamily C, member 5
• Transient receptor protein 5
• TRP5
• TRP-5
• TRP5transient receptor potential channel 5
• TRPC5

Background

1 ug/mL was sufficient for detection of TrpC5 in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRPC5
• Uniprot:
  • NP_036603.1(Human)