Orai1 Antibody (3F6H5) - BSA Free

Images

 
Western Blot: Orai1 Antibody (3F6H5) [NBP1-75522] - analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: Orai1 Antibody (3F6H5) [NBP1-75522] - Human spleen tissue with ORAI1 antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: Orai1 Antibody (3F6H5) [NBP1-75522] - ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 ug/mL
Immunofluorescence: Orai1 Antibody (3F6H5) [NBP1-75522] - ORAI1 in human spleen tissue with ORAI1 antibody at 20 ug/ml.
Immunofluorescence: Orai1 Antibody (3F6H5) [NBP1-75522] - Human spleen tissue with ORAI1 antibody at 20 ug/ml. Red: ORAI1 Antibody [3F6H5] (NBP1-75522). Blue: DAPI staining

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P, ISH, PLA, ICC/IF
Clone
3F6H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Orai1 Antibody (3F6H5) - BSA Free Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.
Specificity
ORAI1 antibody is predicted to have no cross reactivity to other members in the ORAI family.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ORAI1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • In-situ Hybridization
  • Proximity Ligation Assay
  • Western Blot 1-2 ug/ml
Application Notes
Use in In-situ Hybridization reported in scientific literature (PMID: 27535226). Use in Proximity ligation assay reported in scientific literature (PMID: 30018568). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:32199855). ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-75522 in the following applications:

  • 1 publication
  • ISH
    1 publication
  • PLA
    1 publication
  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Orai1 Antibody (3F6H5) - BSA Free

  • CRACM1
  • CRACM1ORAT1
  • FLJ14466
  • ORAI calcium release-activated calcium modulator 1
  • Orai1
  • ORAT1
  • Protein orai-1
  • TMEM142A
  • TMEM142Acalcium release-activated calcium modulator 1
  • Transmembrane protein 142Acalcium release-activated calcium channel protein 1

Background

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Orai1 Antibody (NBP1-75522)(3)

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Bioinformatics

Gene Symbol ORAI1