STIM1 Antibody (5A2)

Images

 
Western Blot: STIM1 Antibody (5A2) [H00006786-M01] - STIM1 monoclonal antibody (M01), clone 5A2. Analysis of STIM1 expression in human liver.
Immunocytochemistry/ Immunofluorescence: STIM1 Antibody (5A2) [H00006786-M01] - Analysis of monoclonal antibody to STIM1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: STIM1 Antibody (5A2) [H00006786-M01] - Analysis of monoclonal antibody to STIM1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. Antibody concentration 3 ...read more
Western Blot: STIM1 Antibody (5A2) [H00006786-M01] - STIM1 expression in HeLa ( Cat # L013V1 ).
Western Blot: STIM1 Antibody (5A2) [H00006786-M01] - STIM1 monoclonal antibody (M01), clone 5A2. Western Blot analysis of STIM1 expression in K-562 ( Cat # L009V1 ).
ELISA: STIM1 Antibody (5A2) [H00006786-M01] - Detection limit for recombinant GST tagged STIM1 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
5A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

STIM1 Antibody (5A2) Summary

Immunogen
STIM1 (AAH21300, 24 a.a. ~ 685 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGAVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK
Specificity
STIM1 (5A2)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
STIM1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. IT has been used for IF, IHC-P, and ELISA. IP usage reported in scientific literature (PMID: 24845346).
Publications
Read Publications using
H00006786-M01 in the following applications:

  • IP
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23076152). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STIM1 Antibody (5A2)

  • GOK
  • GOKD11S4896E
  • STIM1
  • stromal interaction molecule 1

Background

This gene encodes a type 1 transmembrane protein of unknown function. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for STIM1 Antibody (H00006786-M01)(23)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IP.


Filter By Application
IP
(1)
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(1)
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(1)
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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using H00006786-M01 Applications Species
Lisak D, Schacht T, Gawlitza A et al. BAX inhibitor-1 is a Ca2+ channel critically important for immune cell function and survival. Cell Death Differ. 2015 Oct 16 [PMID: 26470731]
Hong CH, Chang KL, Wang HJ et al. IL-9 induces IL-8 production via STIM1 activation and ERK phosphorylation in epidermal keratinocytes: A plausible mechanism of IL-9R in atopic dermatitis. J Dermatol Sci. 2015 Mar 16 [PMID: 25840641]
Duquette M, Nadler M, Okuhara D et al. Members of the thrombospondin gene family bind stromal interaction molecule 1 and regulate calcium channel activity. Matrix Biol. 2014 May 16 [PMID: 24845346] (IP, Human) IP Human
Ferdaus MZ, Xiao B, Ohara H et al. Identification of stim1 as a candidate gene for exaggerated sympathetic response to stress in the stroke-prone spontaneously hypertensive rat. PLoS One. 2014 Apr 15 [PMID: 24736434]
Umemura M, Baljinnyam E, Feske S et al. Store-Operated Ca(2) (+) Entry (SOCE) Regulates Melanoma Proliferation and Cell Migration. PLoS One. 2014 Feb 21 [PMID: 24586666]
Schmid E, Bhandaru M, Nurbaeva MK et al. SGK3 Regulates Ca Entry and Migration of Dendritic Cells. Cell Physiol Biochem. 2012 Nov 22 [PMID: 23171960]
Jans R, Mottram L, Johnson DL et al. Lysophosphatidic Acid Promotes Cell Migration through STIM1- and Orai1-Mediated Ca(2+)(i) Mobilization and NFAT2 Activation. J Invest Dermatol. 2012 Oct 25 [PMID: 23096711]
Henke N, Albrecht P, Pfeiffer A et al. Stromal interaction molecule 1 (STIM1) is involved in the regulation of mitochondrial shape and bioenergetics and plays a role in oxidative stress. J Biol Chem. 2012 Dec 7 [PMID: 23076152] (Mouse) Mouse
Elvers M, Herrmann A, Seizer P et al. Intracellular cyclophilin A is an important Ca2+ regulator in platelets and critically involved in arterial thrombus formation. Blood. 2012 Jun 27 [PMID: 22740452]
Eylenstein A, Schmidt S, Gu S et al. Transcription factor NF-kB regulates the expressions of a pore-forming unit, Orai1, and its activator, STIM1, to control Ca2+ entry and affect cellular functions. J Biol Chem. 2011 Nov 21 [PMID: 22110130]
Show All 23 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol STIM1
Entrez
OMIM
Uniprot