Nephrin Antibody [Unconjugated]

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Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. ...read more
Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Nephrin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Nephrin
Gln37-Thr1049
Accession # NP_062332
Specificity
Detects mouse Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Nephrin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPHS1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF3159 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephrin Antibody [Unconjugated]

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHNCNF
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor

Background

Nephrin is a 185 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse Nephrin consists of a 1042 amino acid (aa) extracellular domain (ECD) with eight Ig-like C2-set domains and one fibronectin type III domain, a 22 aa transmembrane segment, and a 156 aa cytoplasmic tail (2, 3). Within the ECD, mouse Nephrin shares 84% and 95% aa sequence identity with human and rat Nephrin, respectively. Usage of the alternate exon 1B results in a distinct N-terminal sequence that lacks a clearly defined signal peptide cleavage site (4). Nephrin is expressed primarily on podocytes in the renal glomerulus and to a lesser extent in the brain and pancreas (3, 5). The 1B isoform is not expressed in the kidney (4). Nephrin localizes to intercellular junctions between podocyte foot processes where it functions as a homophilic adhesion molecule (2, 6). Nephrin is required for formation and maintenance of the slit diaphragm between these processes (7). It associates with Neph1, podicin, P-cadherin, and multiple scaffolding proteins which couple it to the actin cytoskeleton (8-12). Nephrin expression is required for the anti-apoptotic effect of VEGF on podocytes as well as for the ability of podocytes to upregulate Glut1 and Glut4 glucose transporters in response to insulin (13, 14). Nephrin downregulation contributes to diabetic nephropathy, and Nephrin mutations underlie the lethal congenital nephritic syndrome NPHS1 (5, 15).

  1. Kawachi, H. et al. (2006) Nephrology 11:274.
  2. Holzman, L.B. et al. (1999) Kidney Int. 56:1481.
  3. Putaala, H. et al. (2000) J. Am. Soc. Nephrol. 11:991.
  4. Beltcheva, O. et al. (2003) J. Am. Soc. Nephrol. 14:352.
  5. Putaala, H. et al. (2001) Hum. Mol. Genet. 10:1.
  6. Khoshnoodi, J. et al. (2003) Am. J. Pathol. 163:2337.
  7. Ruotsalainen, V. et al. (2000) Am. J. Pathol. 157:1905.
  8. Barletta, G.M. et al. (2003) J. Biol. Chem. 278:19266.
  9. Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
  10. Lehtonen, S. et al. (2004) Am. J. Pathol. 165:923.
  11. Lehtonen, S. et al. (2005) Proc. Natl. Acad. Sci. USA 102:9814.
  12. Verma, R. et al. (2006) J. Clin. Invest. 116:1346.
  13. Foster, R.R. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F48.
  14. Coward, R.J. et al. (2007) Diabetes 56:1127.
  15. Cooper, M.E. et al. (2002) Semin. Nephrol. 22:393.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nephrin Antibody (AF3159)(29)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(2)
IHC
(16)
IHC-Fr
(3)
IHC-P
(3)
Neutralization
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(2)
Mouse
(26)
Rat
(2)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using AF3159 Applications Species
S Kumar, X Fan, H Milo Rasou, R Sharma, DJ Salant, W Lu ZEB2 controls kidney stromal progenitor differentiation and inhibits abnormal myofibroblast expansion and kidney fibrosis JCI Insight, 2023-01-10;0(0):. 2023-01-10 [PMID: 36445780] (IHC, Mouse) IHC Mouse
MK Kocylowski, H Aypek, W Bildl, M Helmstädte, P Trachte, B Dumoulin, S Wittösch, L Kühne, U Aukschun, C Teetzen, O Kretz, B Gaal, A Kulik, C Antignac, G Mollet, A Köttgen, B Göcmen, J Schwenk, U Schulte, TB Huber, B Fakler, F Grahammer A slit-diaphragm-associated protein network for dynamic control of renal filtration Nature Communications, 2022-10-28;13(1):6446. 2022-10-28 [PMID: 36307401] (IHC, Rat) IHC Rat
A Gupta, K Singh, S Fatima, S Ambreen, S Zimmermann, R Younis, S Krishnan, R Rana, I Gadi, C Schwab, R Biemann, K Shahzad, V Rani, S Ali, PR Mertens, S Kohli, B Isermann Neutrophil Extracellular Traps Promote NLRP3 Inflammasome Activation and Glomerular Endothelial Dysfunction in Diabetic Kidney Disease Nutrients, 2022-07-20;14(14):. 2022-07-20 [PMID: 35889923] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
L Abbad, N Prakoura, A Michon, R Chalghoumi, S Reichelt-W, MC Banas, C Chatzianto Role of Periostin and Nuclear Factor-kappaB Interplay in the Development of Diabetic Nephropathy Cells, 2022-07-15;11(14):. 2022-07-15 [PMID: 35883655] (Western Blot, Mouse) Western Blot Mouse
JH Kim, KH Hwang, BTN Dang, M Eom, ID Kong, Y Gwack, S Yu, HY Gee, L Birnbaumer, KS Park, SK Cha Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria Nature Communications, 2021-11-11;12(1):6537. 2021-11-11 [PMID: 34764278] (IHC, Mouse) IHC Mouse
L Wang, Y Tang, AF Buckley, RF Spurney Blockade of the natriuretic peptide clearance receptor attenuates proteinuria in a mouse model of focal segmental glomerulosclerosis Physiological Reports, 2021-11-01;9(21):e15095. 2021-11-01 [PMID: 34755480] (Western Blot, Mouse, Transgenic Mouse) Western Blot Mouse, Transgenic Mouse
MC de Ponte, VG Cardoso, GL Gonçalves, JM Costa-Pess, M Oliveira-S Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury Scientific Reports, 2021-09-24;11(1):19028. 2021-09-24 [PMID: 34561469] (IHC, Mouse) IHC Mouse
S Ozawa, M Matsubayas, H Nanaura, M Yanagita, K Mori, K Asanuma, N Kajiwara, K Hayashi, H Ohashi, M Kasahara, H Yokoi, H Kataoka, E Mori, T Nakagawa Proteolytic Cleavage of Podocin by Matriptase Exacerbates Podocyte Injury J. Biol. Chem., 2020-09-09;0(0):. 2020-09-09 [PMID: 32907879] (IHC, Mouse) IHC Mouse
R Ganugula, M Arora, D Zou, SK Agarwal, C Mohan, MNVR Kumar A highly potent lymphatic system-targeting nanoparticle cyclosporine prevents glomerulonephritis in mouse model of lupus Sci Adv, 2020;6(24):eabb3900. 2020 [PMID: 32582860] (IHC, Mouse) IHC Mouse
R Ganugula, M Arora, D Zou, SK Agarwal, C Mohan, MNVR Kumar A highly potent lymphatic system-targeting nanoparticle cyclosporine prevents glomerulonephritis in mouse model of lupus Sci Adv, 2020-06-12;6(24):eabb3900. 2020-06-12 [PMID: 32582860] (IHC, Mouse) IHC Mouse
Show All 29 Publications.

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Bioinformatics

Gene Symbol NPHS1
Uniprot