Nephrin Antibody [Unconjugated]


Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. more
Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained more

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Reactivity MuSpecies Glossary
Applications IHC

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Nephrin Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse Nephrin
Accession # NP_062332
Detects mouse Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Nephrin is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
Read Publications using
AF3159 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephrin Antibody [Unconjugated]

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor


Nephrin is a 185 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse Nephrin consists of a 1042 amino acid (aa) extracellular domain (ECD) with eight Ig-like C2-set domains and one fibronectin type III domain, a 22 aa transmembrane segment, and a 156 aa cytoplasmic tail (2, 3). Within the ECD, mouse Nephrin shares 84% and 95% aa sequence identity with human and rat Nephrin, respectively. Usage of the alternate exon 1B results in a distinct N-terminal sequence that lacks a clearly defined signal peptide cleavage site (4). Nephrin is expressed primarily on podocytes in the renal glomerulus and to a lesser extent in the brain and pancreas (3, 5). The 1B isoform is not expressed in the kidney (4). Nephrin localizes to intercellular junctions between podocyte foot processes where it functions as a homophilic adhesion molecule (2, 6). Nephrin is required for formation and maintenance of the slit diaphragm between these processes (7). It associates with Neph1, podicin, P-cadherin, and multiple scaffolding proteins which couple it to the actin cytoskeleton (8-12). Nephrin expression is required for the anti-apoptotic effect of VEGF on podocytes as well as for the ability of podocytes to upregulate Glut1 and Glut4 glucose transporters in response to insulin (13, 14). Nephrin downregulation contributes to diabetic nephropathy, and Nephrin mutations underlie the lethal congenital nephritic syndrome NPHS1 (5, 15).

  1. Kawachi, H. et al. (2006) Nephrology 11:274.
  2. Holzman, L.B. et al. (1999) Kidney Int. 56:1481.
  3. Putaala, H. et al. (2000) J. Am. Soc. Nephrol. 11:991.
  4. Beltcheva, O. et al. (2003) J. Am. Soc. Nephrol. 14:352.
  5. Putaala, H. et al. (2001) Hum. Mol. Genet. 10:1.
  6. Khoshnoodi, J. et al. (2003) Am. J. Pathol. 163:2337.
  7. Ruotsalainen, V. et al. (2000) Am. J. Pathol. 157:1905.
  8. Barletta, G.M. et al. (2003) J. Biol. Chem. 278:19266.
  9. Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
  10. Lehtonen, S. et al. (2004) Am. J. Pathol. 165:923.
  11. Lehtonen, S. et al. (2005) Proc. Natl. Acad. Sci. USA 102:9814.
  12. Verma, R. et al. (2006) J. Clin. Invest. 116:1346.
  13. Foster, R.R. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F48.
  14. Coward, R.J. et al. (2007) Diabetes 56:1127.
  15. Cooper, M.E. et al. (2002) Semin. Nephrol. 22:393.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nephrin Antibody (AF3159)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.

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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using AF3159 Applications Species
JH Kim, KH Hwang, BTN Dang, M Eom, ID Kong, Y Gwack, S Yu, HY Gee, L Birnbaumer, KS Park, SK Cha Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria Nature Communications, 2021;12(1):6537. 2021 [PMID: 34764278] (IHC, Mouse) IHC Mouse
L Wang, Y Tang, AF Buckley, RF Spurney Blockade of the natriuretic peptide clearance receptor attenuates proteinuria in a mouse model of focal segmental glomerulosclerosis Physiological Reports, 2021;9(21):e15095. 2021 [PMID: 34755480] (Western Blot, Mouse) Western Blot Mouse
MC de Ponte, VG Cardoso, GL Gonçalves, JM Costa-Pess, M Oliveira-S Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury Scientific Reports, 2021;11(1):19028. 2021 [PMID: 34561469] (IHC, Mouse) IHC Mouse
S Ozawa, M Matsubayas, H Nanaura, M Yanagita, K Mori, K Asanuma, N Kajiwara, K Hayashi, H Ohashi, M Kasahara, H Yokoi, H Kataoka, E Mori, T Nakagawa Proteolytic Cleavage of Podocin by Matriptase Exacerbates Podocyte Injury J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32907879] (IHC, Mouse) IHC Mouse
HM Kang, JH Lim, KH Noh, D Park, HS Cho, K Susztak, CR Jung Effective reconstruction of functional organotypic kidney spheroid for in vitro nephrotoxicity studies Sci Rep, 2019;9(1):17610. 2019 [PMID: 31772214] (ICC, Mouse) ICC Mouse
X Gao, A Rosales, H Karttunen, GM Bommana, B Tandoh, Z Yi, Z Habib, V Agati, W Zhang, MJ Ross The HIV protease inhibitor darunavir prevents kidney injury via HIV-independent mechanisms Sci Rep, 2019;9(1):15857. 2019 [PMID: 31676833] (IHC-Fr, Mouse) IHC-Fr Mouse
CL Lin, YC Hsu, YT Huang, YH Shih, CJ Wang, WC Chiang, PJ Chang A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction EMBO Mol Med, 2019;11(5):. 2019 [PMID: 30948420] (IHC, Mouse) IHC Mouse
CJ Chang, PC Wang, TC Huang, A Taniguchi Change in Renal Glomerular Collagens and Glomerular Filtration Barrier-Related Proteins in a Dextran Sulfate Sodium-Induced Colitis Mouse Model Int J Mol Sci, 2019;20(6):. 2019 [PMID: 30909435] (IHC-Fr, Mouse) IHC-Fr Mouse
F Li, X Mao, Q Zhuang, Z Zhao, Z Zhang, H Wu Inhibiting 4E-BP1 re-activation represses podocyte cell cycle re-entry and apoptosis induced by adriamycin Cell Death Dis, 2019;10(3):241. 2019 [PMID: 30858353] (ICC, Mouse) ICC Mouse
T Goto, H Hara, M Sanbo, H Masaki, H Sato, T Yamaguchi, S Hochi, T Kobayashi, H Nakauchi, M Hirabayash Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats Nat Commun, 2019;10(1):451. 2019 [PMID: 30723213] (IHC, Mouse) IHC Mouse
Show All 23 Publications.

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Gene Symbol NPHS1