Nephrin Antibody

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Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained ...read more
Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

Nephrin Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Nephrin
Gln37-Thr1049
Accession # NP_062332
Specificity
Detects mouse Nephrin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human Nephrin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPHS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF3159 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephrin Antibody

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHNCNF
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor

Background

Nephrin is a 185 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse Nephrin consists of a 1042 amino acid (aa) extracellular domain (ECD) with eight Ig-like C2-set domains and one fibronectin type III domain, a 22 aa transmembrane segment, and a 156 aa cytoplasmic tail (2, 3). Within the ECD, mouse Nephrin shares 84% and 95% aa sequence identity with human and rat Nephrin, respectively. Usage of the alternate exon 1B results in a distinct N-terminal sequence that lacks a clearly defined signal peptide cleavage site (4). Nephrin is expressed primarily on podocytes in the renal glomerulus and to a lesser extent in the brain and pancreas (3, 5). The 1B isoform is not expressed in the kidney (4). Nephrin localizes to intercellular junctions between podocyte foot processes where it functions as a homophilic adhesion molecule (2, 6). Nephrin is required for formation and maintenance of the slit diaphragm between these processes (7). It associates with Neph1, podicin, P-cadherin, and multiple scaffolding proteins which couple it to the actin cytoskeleton (8-12). Nephrin expression is required for the anti-apoptotic effect of VEGF on podocytes as well as for the ability of podocytes to upregulate Glut1 and Glut4 glucose transporters in response to insulin (13, 14). Nephrin downregulation contributes to diabetic nephropathy, and Nephrin mutations underlie the lethal congenital nephritic syndrome NPHS1 (5, 15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC

Publications for Nephrin Antibody (AF3159)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Flow, IHC, IHC Frozen, IHC Paraffin-embedded, In vivo, WB, Western Blot.


Filter By Application
Flow
(1)
IHC
(4)
IHC Frozen
(1)
IHC Paraffin-embedded
(2)
In vivo
(1)
WB
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(8)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF3159 Applications Species
S Alarcón, W Garrido, C Cappelli, R Suárez, C Oyarzún, C Quezada, R San Martín Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy Sci Rep, 2017;7(1):9439. 2017 [PMID: 28842605] (IHC,, Rat) IHC Rat
Y Kato, K Mori, M Kasahara, K Osaki, A Ishii, KP Mori, N Toda, S Ohno, T Kuwabara, T Tokudome, I Kishimoto, MA Saleem, T Matsusaka, K Nakao, M Mukoyama, M Yanagita, H Yokoi Natriuretic peptide receptor guanylyl cyclase-A pathway counteracts glomerular injury evoked by aldosterone through p38 mitogen-activated protein kinase inhibition Sci Rep, 2017;7(0):46624. 2017 [PMID: 28429785] (IHC,, Mouse) IHC Mouse
SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice J. Immunol, 2017;0(0):. 2017 [PMID: 28219886] (Flow,, Mouse) Flow Mouse
Z Mohammed-A, C Lu, MK Marway, RE Carlisle, K Ask, D Lukic, JC Krepinsky, JG Dickhout Endoplasmic reticulum stress inhibition attenuates hypertensive chronic kidney disease through reduction in proteinuria Sci Rep, 2017;7(0):41572. 2017 [PMID: 28148966] (Western Blot,, Human) Western Blot Human
Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy BMC Nephrol, 2016;17(1):179. 2016 [PMID: 27855638] (In vivo,, Mouse) In vivo Mouse
Sun Y, Qu X, Howard V, Dai L, Jiang X, Ren Y, Fu P, Puelles V, Nikolic-Paterson D, Caruana G, Bertram J, Sleeman M, Li J Smad3 deficiency protects mice from obesity-induced podocyte injury that precedes insulin resistance. Kidney Int, 2015;88(2):286-98. 2015 [PMID: 25945408] (WB,, Mouse) WB Mouse
Kim S, Lee S, Wang Z, Ding Y, Haque N, Zhang J, Zhou J, Choi M TGF-beta-activated kinase 1 is crucial in podocyte differentiation and glomerular capillary formation. J Am Soc Nephrol, 2014;25(9):1966-78. 2014 [PMID: 24652804] (IHC,, Human) IHC Human
Lan, Xiqian, Rai, Partab, Chandel, Nirupama, Cheng, Kang, Lederman, Rivka, Saleem, Moin A, Mathieson, Peter W, Husain, Mohammad, Crosson, John T, Gupta, Kalpna, Malhotra, Ashwani, Singhal, Pravin C Morphine induces albuminuria by compromising podocyte integrity. PLoS ONE, 2013;8(3):e55748. 2013 [PMID: 23555556] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Dane M, van den Berg B, Avramut M, Faas F, van der Vlag J, Rops A, Ravelli R, Koster B, van Zonneveld A, Vink H, Rabelink T Glomerular endothelial surface layer acts as a barrier against albumin filtration. Am J Pathol, 2013;182(5):1532-40. 2013 [PMID: 23518410] (IHC Frozen,, Mouse) IHC Frozen Mouse
Divergent roles of Smad3 and PI3-kinase in murine adriamycin nephropathy indicate distinct mechanisms of proteinuria and fibrogenesis. Kidney Int., 2012;82(5):525-36. 2012 [PMID: 22534961] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Show All 11 Publications.

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Bioinformatics

Gene Symbol NPHS1
Entrez
Uniprot