MITF Antibody

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="MITF was detected in paraffin-embedded sections of human skin using Goat Anti-Human MITF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5769) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

MITF was detected in paraffin-embedded sections of human skin using Goat Anti-Human MITF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5769) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

HAF019). A specific band was detected for MITF at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." > HAF019). A specific band was detected for MITF at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." title="Western blot shows lysates of Bowes human melanoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MITF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5769) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MITF at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." />

Western blot shows lysates of Bowes human melanoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MITF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5769) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MITF Antibody Summary

• Immunogen: E. coli-derived recombinant human MITF
  Val119-Lys289
  Accession # O75030
• Specificity: Detects human MITF in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MITF
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MITF Antibody

• bHLHe32
• bHLHe32MI
• Class E basic helix-loop-helix protein 32
• MI
• microphthalmia-associated transcription factor
• MITF
• Waardenburg syndrome, type 2A
• WS2A

Background

MITF (Microphthalmia-associated transcription factor) is a member of the MiT/TFE family of molecules. Although it has a predicted MW of 58 kDa, it runs anomalously in SDS-PAGE at 57‑66 kDa. MITF-A1 is found in melanocytes and RPEs where it regulates melanin synthesis via tyrosinase and TRP-1 gene expression. Human MITF-A1 is 526 amino acids (aa) in length. It contains a bHLH DNA binding region (aa 309‑369) and a Leu-zipper domain (aa 374‑395). There are three phosphorylation sites at Ser54/405/414, and two SUMOylation sites at Arg289/423. MITF-A1 acts as either a homodimer, or heterodimer with TFE3, TFEB or TFEC. Multiple splice variants exist, with each variant expressing one of two isoforms that are defined by the presence (#1), or absence (#2), of aa 294‑299. One variant has a deletion of aa 139‑194, a second has an 11 aa substitution for aa 1‑118, and three others show variable substitutions over aa 1‑35. Over aa 119‑289, human MITF-A1 shares 96% aa identity with mouse MITF-A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MITF Antibody (AF5769)(12)

Publications using AF5769

• Degefu, YN;Bujnowska, M;Baumann, DG;Fallahi-Sichani, M; Cell state plasticity emerging from co-regulated, competitive, and configurable interactions within the AP-1 network bioRxiv : the preprint server for biology 2025-11-07 [PMID: 41279149] (Immunocytochemistry, Human)
• Oh, D;Hong, N;Eun, K;Lee, J;Cai, L;Kim, M;Choi, H;Jawad, A;Ham, J;Park, MG;Kim, B;Lee, SC;Moon, C;Kim, H;Hyun, SH; Generation of a genetically engineered porcine melanoma model featuring oncogenic control through conditional Cre recombination Scientific reports 2025-01-10 [PMID: 39794352] (Immunohistochemistry, Porcine)
• N Comandante, DG Baumann, M Fallahi-Si AP-1 transcription factor network explains diverse patterns of cellular plasticity in melanoma cells Cell Reports, 2022-08-02;40(5):111147. 2022-08-02 [PMID: 35926467] (ICC/IF, Human)
• Justine M. Renauld, Vibhuti Khan, Martín L. Basch Intermediate Cells of Dual Embryonic Origin Follow a Basal to Apical Gradient of Ingression Into the Lateral Wall of the Cochlea Frontiers in Cell and Developmental Biology 3/8/2022 [PMID: 35372344]
• Sophie Colombo, Valérie Petit, Roselyne Y. Wagner, Delphine Champeval, Ichiro Yajima, Franck Gesbert et al. Stabilization of beta -catenin promotes melanocyte specification at the expense of the Schwann cell lineage Development 1/15/2022 [PMID: 34878101]
• Marketa Kaucka, Bara Szarowska, Michaela Kavkova, Maria Eleni Kastriti, Polina Kameneva, Inga Schmidt et al. Nerve-associated Schwann cell precursors contribute extracutaneous melanocytes to the heart, inner ear, supraorbital locations and brain meninges Cellular and Molecular Life Sciences 8/1/2021 [PMID: 34274976]
• MS Prasad, RM Charney, LJ Patel, MI García-Cas Distinct molecular profile and restricted stem cell potential defines the prospective human cranial neural crest from embryonic stem cell state Stem Cell Research, 2020-11-11;49(0):102086. 2020-11-11 [PMID: 33370869] (ICC, Human)
• Gustavo A. Gomez, Maneeshi S. Prasad, Man Wong, Rebekah M. Charney, Patrick B. Shelar, Nabjot Sandhu et al. WNT/ beta -catenin modulates the axial identity of embryonic stem cell-derived human neural crest Development 1/7/2019 [PMID: 31399472]
• Gustavo A. Gomez, Maneeshi S. Prasad, Man Wong, Rebekah M. Charney, Patrick B. Shelar, Nabjot Sandhu et al. WNT/ beta -catenin modulates the axial identity of embryonic stem cell-derived human neural crest Development 2019-01-07 [PMID: 31399472] (Immunocytochemistry, Human)
• Ruth Halaban, Antonella Bacchiocchi, Robert Straub, Jian Cao, Mario Sznol, Deepak Narayan et al. A novel anti-melanoma SRC-family kinase inhibitor Oncotarget 3/19/2019 [PMID: 31040916]
• Gustavo A. Gomez, Maneeshi S. Prasad, Nabjot Sandhu, Patrick B. Shelar, Alan W. Leung, Martín I. García-Castro Human neural crest induction by temporal modulation of WNT activation Developmental Biology 5/1/2019 [PMID: 30826399]
• Adameyko I, Lallemend F, Furlan A, Zinin N, Aranda S, Kitambi SS, Blanchart A, Favaro R, Nicolis S, Lubke M, Muller T, Birchmeier C, Suter U, Zaitoun I, Takahashi Y, Ernfors P Sox2 and Mitf cross-regulatory interactions consolidate progenitor and melanocyte lineages in the cranial neural crest. Development, 2012-01-01;139(2):397-410. 2012-01-01 [PMID: 22186729] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: MITF
• Uniprot:
  • O75030(Human)