TRF-1 Antibody

Western Blot: TRF-1 Antibody [NB600-1391] - Analysis ofTRF1 in 20 ugs of A) Jurkat and B) 293 cell lysateusing this antibody.

Product Discontinued

TRF-1 Antibody Summary

• Immunogen: Synthetic peptides corresponding to amino acids 321-335 and 406-420 of human TRF1 were used as immunogen.
• Marker: Telomere Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TERF1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 4 - 6 ug/ml
• Control:

  Jurkat Whole Cell Lysate
  293 Whole Cell Lysate
  TRF-1 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for TRF-1 Antibody

• NIMA-interacting protein 2
• PIN-2
• PIN2hTRF1-AS
• Telomeric protein Pin2/TRF1
• telomeric repeat binding factor (NIMA-interacting) 1
• telomeric repeat binding factor 1
• telomeric repeat binding protein 1
• telomeric repeat-binding factor 1
• TERF1
• TRBF1
• TRF1
• TRF-1
• TRF1FLJ41416
• TRFt-TRF1
• TTAGGG repeat-binding factor 1

Background

TRF1 is a negative regulator of telomere length maintenance (6). TRF1 has a number of biochemical similarities to Rap1p, a distantly related DNA-binding protein that functions at telomeres in yeast (5,6). Like Rap1p, TRF1 requires two Myb motifs for DNA binding. Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (4). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats (3,4). Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. Recently, it has been shown that TRF1 expression is significantly elevated in patients with acute lymphoblastic leukemia (ALL) compared to those in acute myeloid leukemia (AML) (2). In this study, TRF1 expression tended to be higher in patients without telomere shortening and in those with hTERT expression suggesting that TRF1 may act to monitor telomere length under the condition of up-regulated telomerase activity in some neoplastic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRF-1 Antibody (NB600-1391)(2)

Publications using NB600-1391

• Nyborg AC, Jansen K, Ladd TB et al. A signal peptide peptidase (SPP) reporter activity assay based on the cleavage of type II membrane protein substrates provides further evidence for an inverted orientation of the SPP active site relative to presenilin. J Biol Chem. 2004-10-08 [PMID: 15252014]
  
  Details:
  Western Blot, Figs 1C, 2C, 2E, 3D: Analysis of a substrate consisting of the N-terminus of ATF6 fused to a transmembrane domain suspectible to SPP cleavage in vitro.
• Nikitina T, Woodcock CL. Closed chromatin loops at the ends of chromosomes. J Cell Biol. 2004-07-19 [PMID: 15249582]

Bioinformatics

• Gene Symbol: TERF1
• Entrez:
  • 7013(Human)
• Uniprot:
  • P54274()