TRAF3IP2 Antibody


Western Blot: TRAF3IP2 Antibody [NB100-56740] - Analysis of CIKS using NB100-56740 on 15 ugs of mouse kidney cell lysate.
Immunohistochemistry-Paraffin: TRAF3IP2 Antibody [NB100-56740] - Analysis of ACT1 in human kidney tissue using ACT1 antibody at 5 ug/ml.
Western Blot: TRAF3IP2 Antibody [NB100-56740] - Analysis of ACT1 in human kidney lysate in the A) absence and B) presence of immunizing peptide using ACT1 antibody at 2 ug/ml.
Simple Western: TRAF3IP2 Antibody [NB100-56740] - Simple Western lane view shows a specific band for TRAF3IP2 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
0.5 mg/ml

Order Details

TRAF3IP2 Antibody Summary

This antibody was developed against a synthetic peptide (QDLPRPLRSREFPQFEP) corresponding to amino acids 225-241 of human TRAF3IP2.
An approx. 60 kDa band is observed.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1-2 ug/ml
  • Simple Western 1:10
  • Immunohistochemistry-Paraffin
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Positive Control
Kidney Lysate (NB820-59231)
Kidney Lysate (NB820-59661)
Kidney Lysate (NB820-59709)
TRAF3IP2 Lysate (NBL1-17243)
Read Publications using
NB100-56740 in the following applications:

  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Predicted to react with Rat. The amino acid sequence used as immunogen for this antibody is 100% homologous in human, 88% in rat, and 82% in mouse.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified

Alternate Names for TRAF3IP2 Antibody

  • ACT1DKFZP586G0522
  • adapter protein CIKS
  • C6orf2
  • C6orf4chromosome 6 open reading frame 5
  • C6orf5DKFZp586G0522
  • C6orf6MGC3581
  • CIKSchromosome 6 open reading frame 2
  • Connection to IKK and SAPK/JNK
  • NFkB-activating protein ACT1
  • Nuclear factor NF-kappa-B activator 1
  • TRAF3 interacting protein 2
  • TRAF3-interacting protein 2


NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (3) have isolated a cDNA coding for a protein named Act1 (NF- B activator 1). Act1/CIKS cDNA codes for a protein containing 574 aa with a predicated molecular mass of 60 kDa. Act1/CIKS protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
Species: Hu, Mu, Rt
Applications: WB, ICC
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, ICC
Species: Hu
Applications: WB
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Rt
Applications: WB, ICC/IF, IHC
Supplier Logo

Publications for TRAF3IP2 Antibody (NB100-56740)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IP, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-56740 Applications Species
Padilla J, Carpenter A, Das ND et al. TRAF3IP2 mediates high glucose-induced endothelin-1 production as well as endothelin-1-induced inflammation in endothelial cells. Am. J. Physiol. Heart Circ. Physiol. 2017 Sep 29 [PMID: 28971844] (WB) WB
Yariswamy M, Yoshida T, Valente AJ et al. Cardiac-Restricted Overexpression of TRAF3 Interacting Protein 2 (TRAF3IP2) Results in Spontaneous Development of Myocardial Hypertrophy, Fibrosis and Dysfunction. J. Biol. Chem. Jul 27 2016 [PMID: 27466370] (WB, Mouse) WB Mouse
Sakamuri SS, Valente AJ, Siddesha JM et al. TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and fibrosis. Mol. Cell. Endocrinol. 2016 Apr 01 [PMID: 27040306] (WB, Mouse)

TRAF3IP2 antibody (Imgenex: IMG-563) was used for WB analysis of lysates from wild type TRAF3IP2 and TRAF3IP2-null mice hearts (Fig 1A). WB was also performed on the lysates from tissues of WT mice treated with salt alone or aldosterone + salt (Fig. 2A;
WB Mouse
Somanna NK, Yariswamy M, Garagliano JM et al. Aldosterone-induced cardiomyocyte growth, and fibroblast migration and proliferation are mediated by TRAF3IP2 Cell. Signal. 2015 Jul 04 [PMID: 26148936] (WB, Mouse)

TRAF3IP2 antibody (Imgenex: IMG-563) was used for WB on lysates of murine cardiomyocytes subjected in-vitro treatment of Aldosterone (Fig. 1A, Fig. 4A) as well as Aldosterone in different different combinations with mineralocorticoid receptors antagonist spironolactone (Fig. 1D) or diphenyleneiodonium (Fig. 2C). The antibody was also used for WB on WT or TRAF3IP2-null cardiomyocytes (Fig. 1F)
WB Mouse
Valente AJ, Irimpen AM, Siebenlist U, Chandrasekar B. OxLDL induces endothelial dysfunction and death via TRAF3IP2: Inhibition by HDL3 and AMPK activators. Free Radic. Biol. Med. 2014 Feb 20 [PMID: 24561578] (WB, IP, Human)

Human aortic endothelial cells (EC), multiple figures. Note: The specificity of the TRAF3IP2 pAb was validated with TRAF3IP2 shRNA by WB and in EC transduced with TRAF3IP2 lentrovial by IP/WB.
WB, IP Human
Valente AJ, Yoshida T, Izadpanah R et al. Interleukin-18 enhances IL-18R/Nox1 binding, and mediates TRAF3IP2-dependent smooth muscle cell migration. Inhibition by simvastatin. Cell Signal. 2013 Jun [PMID: 23541442]
Valente AJ, Yoshida T, Clark RA et al. Advanced oxidation protein products induce cardiomyocyte death via Nox2/Rac1/superoxide-dependent TRAF3IP2/JNK signaling. Free Radic Biol Med. 2013 Jul [PMID: 23453926]

Reviews for TRAF3IP2 Antibody (NB100-56740) (0)

There are no reviews for TRAF3IP2 Antibody (NB100-56740). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TRAF3IP2 Antibody (NB100-56740) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Additional TRAF3IP2 Products

Bioinformatics Tool for TRAF3IP2 Antibody (NB100-56740)

Discover related pathways, diseases and genes to TRAF3IP2 Antibody (NB100-56740). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TRAF3IP2 Antibody (NB100-56740)

Discover more about diseases related to TRAF3IP2 Antibody (NB100-56740).

Pathways for TRAF3IP2 Antibody (NB100-56740)

View related products by pathway.

PTMs for TRAF3IP2 Antibody (NB100-56740)

Learn more about PTMs related to TRAF3IP2 Antibody (NB100-56740).

Research Areas for TRAF3IP2 Antibody (NB100-56740)

Find related products by research area.

Blogs on TRAF3IP2

There are no specific blogs for TRAF3IP2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our TRAF3IP2 Antibody and receive a gift card or discount.


Gene Symbol TRAF3IP2