Human CCL4/MIP-1 beta Quantikine ELISA Kit

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Reactivity HuSpecies Glossary
Applications ELISA

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Human CCL4/MIP-1 beta Quantikine ELISA Kit Summary

The Quantikine Human MIP-1 beta Immunoassay is a 3.0 or 4.5 hour solid phase ELISA designed to measure MIP-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 beta (Act-2 variant) and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant human MIP-1 beta. Resu...lts obtained using natural human MIP-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-1 beta.
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Natural and recombinant human MIP-1 beta
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DMB00.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL4/MIP-1 beta Quantikine ELISA Kit

  • ACT2
  • ACT-2
  • AT744.1
  • C-C motif chemokine 4
  • CCL4
  • chemokine (C-C motif) ligand 4
  • Exodus-3
  • G-26 T-lymphocyte-secreted protein
  • G-26
  • HC21
  • LAG1
  • LAG-1
  • Lymphocyte activation gene 1 protein
  • Macrophage inflammatory protein 1-beta
  • MIP1 beta
  • MIP-1 beta
  • MIP1B
  • MIP1B1
  • MIP-1-beta
  • MIP-1-beta(1-69)
  • PAT 744
  • Protein H400
  • SCYA2
  • SCYA4
  • secreted protein G-26
  • SIS-gamma
  • small inducible cytokine A4 (homologous to mouse Mip-1b)
  • Small-inducible cytokine A4
  • T-cell activation protein 2


CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for CCL4/MIP-1 beta (DMB00)(49)

We have publications tested in 4 confirmed species: Human, Primate, Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee).

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Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using DMB00 Applications Species
S Sengupta, NL Board, F Wu, M Moskovljev, J Douglass, J Zhang, BR Reinhold, J Duke-Cohan, J Yu, MC Reed, Y Tabdili, A Azurmendi, EJ Fray, H Zhang, EH Hsiue, K Jenike, YC Ho, SB Gabelli, KW Kinzler, B Vogelstein, S Zhou, JD Siliciano, S Sadegh-Nas, EL Reinherz, RF Siliciano TCR-mimic bispecific antibodies to target the HIV-1 reservoir Proceedings of the National Academy of Sciences of the United States of America, 2022;119(15):e2123406119. 2022 [PMID: 35394875] (Human) Human
Z Liu, J Liu, T Zhang, L Li, S Zhang, H Jia, Y Xia, M Shi, J Zhang, S Yue, X Chen, J Yu Distinct BTK inhibitors differentially induce apoptosis but similarly suppress chemotaxis and lipid accumulation in mantle cell lymphoma BMC Cancer, 2021;21(1):732. 2021 [PMID: 34174847] (Human) Human
E Pandolfi, N Panera, A Alisi, E Carloni, L Russo, I Campagna, C Rizzo, C Concato, G Linardos, L Piccioni, S Jackson, A Villani, F Midulla, AE Tozzi Cytokine expression patterns in hospitalized children with Bordetella pertussis, Rhinovirus or co-infection Scientific Reports, 2021;11(1):10948. 2021 [PMID: 34040002] (Human) Human
L Avila, ML Neves, AR Abreu, CR Fiuza, L Fukusawa, N Meziat-Fil, AR Soares San Cognitive functional therapy (CFT) compared with core training exercise (CTE) in patients with failed back surgery syndrome (FBSS): A study protocol for a randomized controlled trial Journal of bodywork and movement therapies, 2021;26(0):428-434. 2021 [PMID: 33992278] (Human) Human
M Sivina, L Xiao, E Kim, A Vaca, SS Chen, MJ Keating, A Ferrajoli, Z Estrov, N Jain, WG Wierda, X Huang, N Chiorazzi, JA Burger CXCL13 plasma levels function as a biomarker for disease activity in patients with chronic lymphocytic leukemia Leukemia, 2020;0(0):. 2020 [PMID: 33087831] (Human) Human
A Hackel, A Aksamit, K Bruderek, S Lang, S Brandau TNF-alpha and IL-1beta sensitize human MSC for IFN-gamma signalling and enhance neutrophil recruitment Eur. J. Immunol., 2020;0(0):. 2020 [PMID: 32845509] (Human) Human
L Sadeghi, G Arvidsson, M Merrien, A M Wasik, A Görgens, CIE Smith, B Sander, AP Wright Differential B-Cell Receptor Signaling Requirement for Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells Cancers (Basel), 2020;12(5):. 2020 [PMID: 32370190] (Human) Human
Y Lu, BK Yoo, AHC Ng, J Kim, S Yeom, J Tang, MM Lin, AH Zewail, JR Heath 4D electron microscopy of T cell activation Proc. Natl. Acad. Sci. U.S.A., 2019;116(44):22014-22019. 2019 [PMID: 31611413] (Human) Human
N Aziz, R Detels, JJ Quint, D Gjertson, T Ryner, AW Butch Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests BMC Immunol., 2019;20(1):33. 2019 [PMID: 31521107] (Human) Human
E Bournazou, J Samuels, H Zhou, S Krasnokuts, J Patel, T Han, J Bencardino, L Rybak, SB Abramson, U Junker, KS Brown, M Attur Vascular Adhesion Protein-1 (VAP-1) as Predictor of Radiographic Severity in Symptomatic Knee Osteoarthritis in the New York University Cohort Int J Mol Sci, 2019;20(11):. 2019 [PMID: 31146362] (Human) Human
Show All 49 Publications.

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