Human CCL4/MIP-1 beta Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CCL4/MIP-1 beta Quantikine ELISA Kit Summary

• Background: The Quantikine Human MIP-1 beta Immunoassay is a 3.0 or 4.5 hour solid phase ELISA designed to measure MIP-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 beta (Act-2 variant) and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant human MIP-1 beta. Resu...lts obtained using natural human MIP-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-1 beta.
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• Specificity: Natural and recombinant human MIP-1 beta
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL4/MIP-1 beta Quantikine ELISA Kit

• ACT2
• ACT-2
• AT744.1
• C-C motif chemokine 4
• CCL4
• chemokine (C-C motif) ligand 4
• Exodus-3
• G-26 T-lymphocyte-secreted protein
• G-26
• HC21
• LAG1
• LAG-1
• Lymphocyte activation gene 1 protein
• Macrophage inflammatory protein 1-beta
• MIP1 beta
• MIP-1 beta
• MIP1B
• MIP1B1
• MIP-1-beta
• MIP-1-beta(1-69)
• PAT 744
• Protein H400
• SCYA2
• SCYA4
• secreted protein G-26
• SIS-gamma
• small inducible cytokine A4 (homologous to mouse Mip-1b)
• Small-inducible cytokine A4
• T-cell activation protein 2

Background

CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL4/MIP-1 beta (DMB00)(49)

Publications using DMB00

• S Sengupta, NL Board, F Wu, M Moskovljev, J Douglass, J Zhang, BR Reinhold, J Duke-Cohan, J Yu, MC Reed, Y Tabdili, A Azurmendi, EJ Fray, H Zhang, EH Hsiue, K Jenike, YC Ho, SB Gabelli, KW Kinzler, B Vogelstein, S Zhou, JD Siliciano, S Sadegh-Nas, EL Reinherz, RF Siliciano TCR-mimic bispecific antibodies to target the HIV-1 reservoir Proceedings of the National Academy of Sciences of the United States of America, 2022;119(15):e2123406119. 2022 [PMID: 35394875] (Human)
• Z Liu, J Liu, T Zhang, L Li, S Zhang, H Jia, Y Xia, M Shi, J Zhang, S Yue, X Chen, J Yu Distinct BTK inhibitors differentially induce apoptosis but similarly suppress chemotaxis and lipid accumulation in mantle cell lymphoma BMC Cancer, 2021;21(1):732. 2021 [PMID: 34174847] (Human)
• E Pandolfi, N Panera, A Alisi, E Carloni, L Russo, I Campagna, C Rizzo, C Concato, G Linardos, L Piccioni, S Jackson, A Villani, F Midulla, AE Tozzi Cytokine expression patterns in hospitalized children with Bordetella pertussis, Rhinovirus or co-infection Scientific Reports, 2021;11(1):10948. 2021 [PMID: 34040002] (Human)
• L Avila, ML Neves, AR Abreu, CR Fiuza, L Fukusawa, N Meziat-Fil, AR Soares San Cognitive functional therapy (CFT) compared with core training exercise (CTE) in patients with failed back surgery syndrome (FBSS): A study protocol for a randomized controlled trial Journal of bodywork and movement therapies, 2021;26(0):428-434. 2021 [PMID: 33992278] (Human)
• M Sivina, L Xiao, E Kim, A Vaca, SS Chen, MJ Keating, A Ferrajoli, Z Estrov, N Jain, WG Wierda, X Huang, N Chiorazzi, JA Burger CXCL13 plasma levels function as a biomarker for disease activity in patients with chronic lymphocytic leukemia Leukemia, 2020;0(0):. 2020 [PMID: 33087831] (Human)
• A Hackel, A Aksamit, K Bruderek, S Lang, S Brandau TNF-alpha and IL-1beta sensitize human MSC for IFN-gamma signalling and enhance neutrophil recruitment Eur. J. Immunol., 2020;0(0):. 2020 [PMID: 32845509] (Human)
• L Sadeghi, G Arvidsson, M Merrien, A M Wasik, A Görgens, CIE Smith, B Sander, AP Wright Differential B-Cell Receptor Signaling Requirement for Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells Cancers (Basel), 2020;12(5):. 2020 [PMID: 32370190] (Human)
• Y Lu, BK Yoo, AHC Ng, J Kim, S Yeom, J Tang, MM Lin, AH Zewail, JR Heath 4D electron microscopy of T cell activation Proc. Natl. Acad. Sci. U.S.A., 2019;116(44):22014-22019. 2019 [PMID: 31611413] (Human)
• N Aziz, R Detels, JJ Quint, D Gjertson, T Ryner, AW Butch Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests BMC Immunol., 2019;20(1):33. 2019 [PMID: 31521107] (Human)
• E Bournazou, J Samuels, H Zhou, S Krasnokuts, J Patel, T Han, J Bencardino, L Rybak, SB Abramson, U Junker, KS Brown, M Attur Vascular Adhesion Protein-1 (VAP-1) as Predictor of Radiographic Severity in Symptomatic Knee Osteoarthritis in the New York University Cohort Int J Mol Sci, 2019;20(11):. 2019 [PMID: 31146362] (Human)
• N Zhang, G Zhang, N Liu, W Lin, S Ji, M Zheng, K Chen, X Liang, G Li, Y Ma, J Zhu, T Niu, LL Li, J Li, YQ Wei, SY Yang A novel orally available Syk/Src/Jak2 inhibitor, SKLB-850, showed potent anti-tumor activities in B cell lymphoma (BCL) models Oncotarget, 2017;8(67):111495-111507. 2017 [PMID: 29340070] (Human)
• NA Johdi, L Mazlan, I Sagap, R Jamal Profiling of cytokines, chemokines and other soluble proteins as a potential biomarker in colorectal cancer and polyps Cytokine, 2017;99(0):35-42. 2017 [PMID: 28689023] (Human)
• Functional Differences between IgM and IgD Signaling in Chronic Lymphocytic Leukemia J Immunol, 2016;0(0):. 2016 [PMID: 27534555] (Human)
• Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions Cytokine, 2016;84(0):17-24. 2016 [PMID: 27208752] (Human)
• Sadeghi M, Lahdou I, Oweira H, Daniel V, Terness P, Schmidt J, Weiss K, Longerich T, Schemmer P, Opelz G, Mehrabi A Serum levels of chemokines CCL4 and CCL5 in cirrhotic patients indicate the presence of hepatocellular carcinoma. Br J Cancer, 2015;113(5):756-62. 2015 [PMID: 26270232] (Human)
• Balakrishnan K, Peluso M, Fu M, Rosin N, Burger J, Wierda W, Keating M, Faia K, O'Brien S, Kutok J, Gandhi V The phosphoinositide-3-kinase (PI3K)-delta and gamma inhibitor, IPI-145 (Duvelisib), overcomes signals from the PI3K/AKT/S6 pathway and promotes apoptosis in CLL. Leukemia, 2015;29(9):1811-22. 2015 [PMID: 25917267] (Human)
• Pundir P, Catalli A, Leggiadro C, Douglas S, Kulka M Pleurocidin, a novel antimicrobial peptide, induces human mast cell activation through the FPRL1 receptor. Mucosal Immunol, 2014;7(1):177-87. 2014 [PMID: 23839065] (Human)
• de-Oliveira-Pinto LM, Marinho CF, Povoa TF, de Azeredo EL, de Souza LA, Barbosa LD, Motta-Castro AR, Alves AM, Avila CA, de Souza LJ, da Cunha RV, Damasco PV, Paes MV, Kubelka CF Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue Fever. PLoS ONE, 2012;7(7):e38527. 2012 [PMID: 22815692] (Human)
• Ponader S, Chen SS, Buggy JJ The Bruton tyrosine kinase inhibitor PCI-32765 thwarts chronic lymphocytic leukemia cell survival and tissue homing in vitro and in vivo. Blood, 2012;119(5):1182-9. 2012 [PMID: 22180443] (Human)
• Johnson TR, McLellan JS, Graham BS Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. J. Virol., 2012;86(3):1339-47. 2012 [PMID: 22090124] (Human)
• Badoux XC, Keating MJ, Wen S Lenalidomide as initial therapy of elderly patients with chronic lymphocytic leukemia. Blood, 2011;118(13):3489-98. 2011 [PMID: 21725050] (Human)
• Sivina M, Hartmann E, Kipps TJ, Rassenti L, Krupnik D, Lerner S, LaPushin R, Xiao L, Huang X, Werner L, Neuberg D, Kantarjian H, O'Brien S, Wierda WG, Keating MJ, Rosenwald A, Burger JA CCL3 (MIP-1alpha) plasma levels and the risk for disease progression in chronic lymphocytic leukemia. Blood, 2011;117(5):1662-9. 2011 [PMID: 21115978] (Human)
• Tatara Y, Ohishi M, Yamamoto K, Shiota A, Hayashi N, Iwamoto Y, Takeda M, Takagi T, Katsuya T, Ogihara T, Rakugi H Macrophage inflammatory protein-1beta induced cell adhesion with increased intracellular reactive oxygen species. J. Mol. Cell. Cardiol., 2009;47(1):104-11. 2009 [PMID: 19328808] (Human)
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008;181(11):8053-67. 2008 [PMID: 19017998] (Human)
• Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes. J. Immunol., 2008;181(9):5857-64. 2008 [PMID: 18941174] (Human)
• Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22. J. Interferon Cytokine Res., 2008;28(5):297-310. 2008 [PMID: 18547160] (Human)
• Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B Duffy antigen modifies the chemokine response in human endotoxemia. Crit. Care Med., 2008;36(1):159-65. 2008 [PMID: 18007272] (Human)
• Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions. Int. J. Cancer, 2008;122(4):769-76. 2008 [PMID: 17935139] (Human)
• Bolovan-Fritts CA, Spector SA Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction. Blood, 2007;111(1):175-82. 2007 [PMID: 17895402] (Human)
• Bolovan-Fritts CA, Trout RN, Spector SA High T-cell response to human cytomegalovirus induces chemokine-mediated endothelial cell damage. Blood, 2007;110(6):1857-63. 2007 [PMID: 17519388] (Human)
• Benson M, Langston MA, Adner M, Andersson B, Torinssson-Naluai A, Cardell LO A network-based analysis of the late-phase reaction of the skin. J. Allergy Clin. Immunol., 2006;118(1):220-5. 2006 [PMID: 16815159] (Human)
• Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES. Int. Immunol., 2006;18(8):1243-51. 2006 [PMID: 16740603] (Human)
• Kristjansson S, Bjarnarson SP, Wennergren G, Palsdottir AH, Arnadottir T, Haraldsson A, Jonsdottir I Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response. J. Allergy Clin. Immunol., 2005;116(4):805-11. 2005 [PMID: 16210054] (Human)
• Capelli A, Di Stefano A, Gnemmi I, Donner CF CCR5 expression and CC chemokine levels in idiopathic pulmonary fibrosis. Eur. Respir. J., 2005;25(4):701-7. 2005 [PMID: 15802346] (Human)
• Grygorczuk S, Pancewicz S, Zajkowska J, Kondrusik M, Rwierzbinska R, Hermanowska-Szpakowicz T Concentrations of macrophage inflammatory proteins MIP-1alpha and MIP-1beta and interleukin 8 (il-8) in lyme borreliosis. Infection, 2004;32(6):350-5. 2004 [PMID: 15597225] (Human)
• Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages. J. Gen. Virol., 2004;85(0):2603-13. 2004 [PMID: 15302954] (Human)
• Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells. J. Leukoc. Biol., 2004;75(3):504-14. 2004 [PMID: 14742635] (Human)
• Burton CT, »V6þÌ™+ìZ, Hardy GA, Sullivan AK, Nelson MR, Gazzard B, Gotch FM, Imami N Impact of NNRTI compared to PI-based highly active antiretroviral therapy on CCR5 receptor expression, beta-chemokines and IL-16 secretion in HIV-1 infection. , 2002;130(2):286-92. 2002 [PMID: 12390317] (Human)
• Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells. J. Immunol., 2002;169(8):4172-82. 2002 [PMID: 12370346] (Human)
• D'Ambrosio D Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes. Eur. J. Immunol., 2002;32(10):2934-43. 2002 [PMID: 12355447] (Human)
• Honczarenko M, Le Y, Glodek AM, Silberstein LE CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor. Blood, 2002;100(7):2321-9. 2002 [PMID: 12239139] (Human)
• Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion. Eur. J. Immunol., 2002;32(7):2037-45. 2002 [PMID: 12115625] (Human)
• Milliken D, Scotton C, Raju S, Balkwill F, Wilson J Analysis of chemokines and chemokine receptor expression in ovarian cancer ascites. Clin. Cancer Res., 2002;8(4):1108-14. 2002 [PMID: 11948121] (Human)
• Binninger-Schinzel D, 2019, Norley S, Adler HS, Oberg HH, Kurth R Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes. , 2002;294(1):209-21. 2002 [PMID: 11886279] (Human)
• Yates SL, Burgess LH, Kocsis-Angle J, Antal JM, Dority MD, Embury PB, Piotrkowski AM, Brunden KR Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia. J. Neurochem., 2000;74(3):1017-25. 2000 [PMID: 10693932] (Human)
• Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees. J. Infect. Dis., 1999;179(3):646-52. 1999 [PMID: 9952371] (Primate - Pan troglodytes (Chimpanzee))
• Gauduin MC, Glickman RL, Ahmad S, Yilma T, Johnson RP Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and beta-chemokine production. Proc. Natl. Acad. Sci. U.S.A., 1999;96(24):14031-6. 1999 [PMID: 10570193] (Primate)
• Lehner T, —îÊëÖ“§, r¹oŽ&A½ýx, Wang Y, Ïï§Ù´âoÑ‘, Cranage M, Bergmeier LA, Mitchell E, Tao L, Hall G, Dennis M, Cook N, Brookes R, Klavinskis L, Jones I, Doyle C, Ward R Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques. , 1996;2(7):767-75. 1996 [PMID: 8673922] (Primate - Macaca mulatta (Rhesus Macaque))

Bioinformatics

• Gene Symbol: CCL4