Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit



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Reactivity HuSpecies Glossary
Applications ELISA

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Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit Summary

The Quantikine Human CD40 Ligand Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CD40 Ligand in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human CD40 Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD40 Ligand showed linear curves that were parallel to the... standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD40 Ligand.
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Natural and recombinant human CD40 Ligand
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DCDL40.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit

  • CD154 antigen
  • CD154
  • CD40 antigen ligand
  • CD40 Ligand
  • CD40L
  • CD40-L
  • CD40LG
  • CD40LIGM
  • gp39
  • hCD40L
  • HIGM1
  • T-B cell-activating molecule
  • T-BAM
  • T-cell antigen Gp39
  • TNF-related activation protein
  • TNFSF5
  • TRAP
  • TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
  • tumor necrosis factor (ligand) superfamily member 5
  • Tumor necrosis factor ligand superfamily member 5


CD40 Ligand, also known as TNFSF5, CD154, TRAP, and gp39, is a trimeric protein that is expressed in transmembrane and soluble forms. It is expressed by many immune cell types as well as by endothelial and smooth muscle cells of the vasculature. It binds to CD40 on antigen presenting cells, platelets, and endothelial and epithelial cells, leading to enhanced B cell activation and T cell dependent humoral immune responses. CD40 Ligand dysregulation contributes to immune pathology in AIDS, atherosclerosis, atherothrombosis, and restenosis.
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Publications for CD40 Ligand/TNFSF5 (DCDL40)(44)

We have publications tested in 1 confirmed species: Human.

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Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using DCDL40 Applications Species
Juhász, L;L?rincz, H;Szentpéteri, A;Tóth, N;Varga, É;Paragh, G;Harangi, M; Decreased Serum Stromal Cell-Derived Factor-1 in Patients with Familial Hypercholesterolemia and Its Strong Correlation with Lipoprotein Subfractions International journal of molecular sciences 2023-10-18 [PMID: 37894988] (Human) Human
K Buschmann, Y Gramlich, R Chaban, M Oelze, U Hink, T Münzel, H Treede, A Daiber, GD Duerr Disturbed Lipid Metabolism in Diabetic Patients with Manifest Coronary Artery Disease Is Associated with Enhanced Inflammation International Journal of Environmental Research and Public Health, 2021-10-17;18(20):. 2021-10-17 [PMID: 34682638] (Human) Human
S Sendic, L Mansouri, S Lundberg, A Nopp, SH Jacobson, J Lundahl B cell and monocyte phenotyping: A quick asset to investigate the immune status in patients with IgA nephropathy PLoS ONE, 2021-03-19;16(3):e0248056. 2021-03-19 [PMID: 33740017] (Human) Human
N Aziz, R Detels, JJ Quint, D Gjertson, T Ryner, AW Butch Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests BMC Immunol., 2019-09-14;20(1):33. 2019-09-14 [PMID: 31521107] (Human) Human
T Sun, M Ju, X Dai, H Dong, W Gu, Y Gao, R Fu, X Liu, Y Huang, W Liu, Y Chi, W Wang, H Li, Y Zhou, L Shi, R Yang, L Zhang Multilevel defects in the hematopoietic niche in essential thrombocythemia Haematologica, 2019-07-09;0(0):. 2019-07-09 [PMID: 31289202] (Human) Human
DE Martínez-F, JR Padilla-Gu, F Casillas-M, E Valdés-Alv, B Parra-Reyn, M Aceves-Ram, JF Muñoz-Vall, U Zalapa Flo, JC Chávez Her, Y Valle Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome J Immunol Res, 2019-04-30;2019(0):8063983. 2019-04-30 [PMID: 31183392] (Human) Human
ZL Liu, J Hu, XF Xiao, Y Peng, SP Zhao, XZ Xiao, MS Yang The CD40 rs1883832 Polymorphism Affects Sepsis Susceptibility and sCD40L Levels Biomed Res Int, 2018-03-26;2018(0):7497314. 2018-03-26 [PMID: 29780830] (Human) Human
CD Ruediger, B John, S Kumar, HS Lim, G Rangnekar, KC Roberts-Th, GD Young, D Chase, P Sanders, SR Willoughby Influence of ethnic background on left atrial markers of inflammation, endothelial function and tissue remodelling Indian Pacing Electrophysiol J, 2017-08-30;18(1):1-5. 2017-08-30 [PMID: 29477215] (Human) Human
A Szentpéter, N Zsíros, VE Varga, H L?rincz, M Katkó, I Seres, P Fülöp, G Paragh, M Harangi Paraoxonase-1 and myeloperoxidase correlate with vascular biomarkers in overweight patients with newly diagnosed untreated hyperlipidaemia VASA, 2017-06-12;0(0):1-7. 2017-06-12 [PMID: 28602123] (Human) Human
L Lorente, MM Martín, A Pérez-Ceja, J Ferreres, J Solé-Violá, L Labarta, C Díaz, A Jiménez Non-survivor septic patients have persistently higher serum sCD40L levels than survivors J Crit Care, 2017-05-24;41(0):177-182. 2017-05-24 [PMID: 28570959] (Human) Human
Show All 44 Publications.

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FAQs for CD40 Ligand/TNFSF5 (DCDL40). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
    • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at with any questions regarding this program.

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Gene Symbol CD40LG