TRAF-2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NBP1-86914] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP1-86914] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Western Blot: TRAF-2 Antibody [NBP1-86914] - Analysis using Anti-TRAF2 antibody NBP1-86914 (A) shows similar pattern to independent antibody NBP1-86913 (B).

Analysis in control (vector only transfected HEK293T lysate) and TRAF2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRAF-2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEME
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  TRAF-2 Protein (NBP1-86914PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TRAF-2 Antibody - BSA Free

• E3 ubiquitin-protein ligase TRAF2
• EC 6.3.2.-
• MGC:45012
• TNF receptor-associated factor 2
• TRAF2
• TRAF-2
• TRAP3
• TRAP3TRAP
• tumor necrosis factor type 2 receptor associated protein 3
• Tumor necrosis factor type 2 receptor-associated protein 3

Background

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. TRAF2 is typically described as a 501 amino acid (aa) protein, GenBank no. NP_066961.2. However, various TRAF2 isoforms have been described. Therefore the molecular weight of TRAF2 detected by Western blot may vary depending on isoforms expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-2 Antibody (NBP1-86914)(1)

Publication using NBP1-86914

• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).

Bioinformatics

• Gene Symbol: TRAF2
• Entrez:
  • 7186(Human)
• Uniprot:
  • Q12933()