| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56173SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3277-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56715SS | |||
| Azide and BSA Free | NBP2-80987 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56172SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61661-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86913-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77248-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03438-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3277-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86914-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26271-50ul | |||
![Western Blot: TRAF-2 Antibody [NB100-56173] - Analysis of TRAF-2 in normal human tissues using this antibody. TRAF-2 is observed at ~60-64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-2 degradation fragments.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NB100-56173-img0008.jpg "Western Blot: TRAF-2 Antibody [NB100-56173] - Analysis of TRAF-2 in normal human tissues using this antibody. TRAF-2 is observed at ~60-64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-2 degradation fragments.")
![Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NB100-56173] - Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfectionMCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies. Image collected and cropped by Citeab from the following publication (A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma. Oncotarget (2011) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56173-img0009.jpg)
![Immunohistochemistry-Paraffin: TRAF-2 Antibody [NB100-56173] - Formalin-fixed, paraffin-embedded human colon carcinoma tissue sections stained for TRAF2 using this antibody at 1:2000. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-Paraffin-NB100-56173-img0007.jpg)
![Western Blot: TRAF-2 Antibody [NB100-56173] - Detection of TRAF-2 protein in various tumor cell lines using this antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NB100-56173-img0005.jpg)
![Immunohistochemistry: TRAF-2 Antibody [NB100-56173] - Formalin-fixed, paraffin-embedded human melanoma tissue section stained for TRAF2 at 1:2000. A1 is a higher magnification of A. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-NB100-56173-img0006.jpg)
![Western Blot: TRAF-2 Antibody [NB100-56173] - Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated & HN30 cells transfected with pcDNA 3.0 NSP 5a3a & pcDNA 3.1/CT-GFP for 3 daysNT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a, & f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.306), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56173_rabbit-polyclonal-traf-2-antibody-31020241552526.jpg)
![Western Blot: TRAF-2 Antibody (33A1293) [NB100-56715] - Analysis of TRAF2 in HeLa lysate using TRAF2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-33A1293-Western-Blot-NB100-56715-img0004.jpg "Western Blot: TRAF-2 Antibody (33A1293) [NB100-56715] - Analysis of TRAF2 in HeLa lysate using TRAF2 antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: TRAF-2 Antibody (33A1293) [NB100-56715] - Human transitional cell carcinoma of the urinary bladder stained with TRAF2 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-33A1293-Immunohistochemistry-Paraffin-NB100-56715-img0005.jpg)
![Immunohistochemistry-Paraffin: TRAF-2 Antibody [NB100-56172] - Analysis of TRAF-2 in formalin-fixed, paraffin-embedded normal human thymus sections using NB100-56172 at 1:2000. A-C, successively higher magnifications. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-Paraffin-NB100-56172-img0006.jpg "Immunohistochemistry-Paraffin: TRAF-2 Antibody [NB100-56172] - Analysis of TRAF-2 in formalin-fixed, paraffin-embedded normal human thymus sections using NB100-56172 at 1:2000. A-C, successively higher magnifications. Hematoxylin-eosin counterstain.")
![Immunohistochemistry: TRAF-2 Antibody [NB100-56172] - Analysis of TRAF2 in formalin-fixed, paraffin-embedded normal human bone marrow section using NB100-56172 at 1:2000. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-NB100-56172-img0005.jpg)
![Western Blot: TRAF-2 Antibody (5C2C3) [NBP2-61661] - Analysis using TRAF2 mAb against human TRAF2 (AA: 39-188) recombinant protein. (Expected MW is 42.5 kDa)](http://images.novusbio.com/fullsize/TRAF-2-Antibody-5C2C3-Western-Blot-NBP2-61661-img0006.jpg "Western Blot: TRAF-2 Antibody (5C2C3) [NBP2-61661] - Analysis using TRAF2 mAb against human TRAF2 (AA: 39-188) recombinant protein. (Expected MW is 42.5 kDa)")
![Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody (5C2C3) [NBP2-61661] - Analysis of MCF-7 cells using TRAF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-5C2C3-Immunocytochemistry-Immunofluorescence-NBP2-61661-img0004.jpg)
![Immunohistochemistry: TRAF-2 Antibody (5C2C3) [NBP2-61661] - Analysis of paraffin-embedded cervical cancer tissues using TRAF2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-5C2C3-Immunohistochemistry-NBP2-61661-img0005.jpg)
![Flow Cytometry: TRAF-2 Antibody (5C2C3) [NBP2-61661] - Analysis of HepG2 cells using TRAF-2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TRAF-2-Antibody-5C2C3-Flow-Cytometry-NBP2-61661-img0008.jpg)
![Western Blot: TRAF-2 Antibody (5C2C3) [NBP2-61661] - Analysis using TRAF2 mAb against HEK293 (1) and TRAF2 (AA: 39-188)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-5C2C3-Western-Blot-NBP2-61661-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody (5C2C3) [NBP2-61661] - Analysis of HL-7702 cells using TRAF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-5C2C3-Immunocytochemistry-Immunofluorescence-NBP2-61661-img0003.jpg)
![Flow Cytometry: TRAF-2 Antibody (5C2C3) [NBP2-61661] - Analysis of Hela cells using TRAF2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TRAF-2-Antibody-5C2C3-Flow-Cytometry-NBP2-61661-img0002.jpg)
![ELISA: TRAF-2 Antibody (5C2C3) [NBP2-61661] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/TRAF-2-Antibody-5C2C3-ELISA-NBP2-61661-img0001.jpg)
![Western Blot: TRAF-2 Antibody [NBP1-86913] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NBP1-86913-img0008.jpg "Western Blot: TRAF-2 Antibody [NBP1-86913] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NBP1-86913] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86913-img0007.jpg)
![Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP1-86913] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86913-img0006.jpg)
![Western Blot: TRAF-2 Antibody [NBP1-86913] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NBP1-86913-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TRAF-2 Antibody [NBP1-86913] - Analysis using Anti-TRAF2 antibody NBP1-86913 (A) shows similar pattern to independent antibody NBP1-86914 (B).](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NBP1-86913-img0009.jpg)
![Western Blot: TRAF-2 Antibody [NBP1-77248] - Western blot analysis of TRAF2 in human liver tissue lysate with TRAF2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NBP1-77248-img0004.jpg "Western Blot: TRAF-2 Antibody [NBP1-77248] - Western blot analysis of TRAF2 in human liver tissue lysate with TRAF2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NBP1-77248] - Immunofluorescence of TRAF2 in Hela cells with TRAF2 antibody at 20 ug/mL. Green: TRAF2 Antibody (NBP1-77248) Blue: DAPI staining](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunofluorescence-NBP1-77248-img0009.jpg)
![Immunohistochemistry: TRAF-2 Antibody - BSA Free [NBP1-77248] - Immunohistochemistry of TRAF-2 in human liver tissue with TRAF-2 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77248_rabbit-polyclonal-traf-2-antibody-2711202420272456.jpg)
![Immunocytochemistry/ Immunofluorescence: TRAF-2 Antibody - BSA Free [NBP1-77248] - Immunofluorescence of TRAF-2 in human liver tissue with TRAF-2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77248_rabbit-polyclonal-traf-2-antibody-271120242016948.jpg)
![Western Blot: TRAF-2 Antibody [NBP3-03438] - Analysis of extracts of various cell lines, using TRAF-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NBP3-03438-img0003.jpg "Western Blot: TRAF-2 Antibody [NBP3-03438] - Analysis of extracts of various cell lines, using TRAF-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP3-03438] - Immunohistochemistry of paraffin-embedded rat testis using [KO Validated] TRAF-2 Rabbit pAb (NBP3-03438) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-Paraffin-NBP3-03438-img0007.jpg)
![Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP3-03438] - Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] TRAF-2 Rabbit pAb (NBP3-03438) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-Paraffin-NBP3-03438-img0004.jpg)
![Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP3-03438] - Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] TRAF-2 Rabbit pAb (NBP3-03438) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-Paraffin-NBP3-03438-img0005.jpg)
![Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP3-03438] - Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] TRAF-2 Rabbit pAb (NBP3-03438) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-Paraffin-NBP3-03438-img0006.jpg)
![Western Blot: TRAF-2 Antibody [NBP1-86914] - Analysis in control (vector only transfected HEK293T lysate) and TRAF2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NBP1-86914-img0012.jpg "Western Blot: TRAF-2 Antibody [NBP1-86914] - Analysis in control (vector only transfected HEK293T lysate) and TRAF2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NBP1-86914] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86914-img0011.jpg)
![Immunohistochemistry-Paraffin: TRAF-2 Antibody [NBP1-86914] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86914-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TRAF-2 Antibody [NBP1-86914] - Analysis using Anti-TRAF2 antibody NBP1-86914 (A) shows similar pattern to independent antibody NBP1-86913 (B).](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NBP1-86914-img0013.jpg)
![Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of lysates from wild type(WT) and TRAF-2 knockdown (KD) 293T cells, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15682_rabbit-traf-2-mab-3s2a8-522025173453.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of lysates from wild type(WT) and TRAF-2 knockdown (KD) 293T cells, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of various lysates, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15682_rabbit-traf-2-mab-3s2a8-52202517304918.jpg)
![Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of various lysates, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15682_rabbit-traf-2-mab-3s2a8-5220251736129.jpg)
![Immunoprecipitation: TRAF-2 Antibody (3S2A8) [TRAF-2] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug TRAF-2 antibody . Western blot was performed from the immunoprecipitate using TRAF-2 antibody at a dilution of 1:50000.](http://images.novusbio.com/fullsize/nbp3-15682_rabbit-traf-2-mab-3s2a8-52202517361223.jpg)
![Immunoprecipitation: TRAF-2 Antibody (9A5) [NBP3-26271] - Immunoprecipitating TRAF-2 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26271 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26271 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26271_rabbit-traf-2-mab-9a5-26220241322157.jpg "Immunoprecipitation: TRAF-2 Antibody (9A5) [NBP3-26271] - Immunoprecipitating TRAF-2 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26271 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26271 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] - Image of TRAF-2 Antibody (9A5) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26271_rabbit-traf-2-mab-9a5-262202414471589.jpg)
![Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] - Image of TRAF-2 Antibody (9A5) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26271_rabbit-traf-2-mab-9a5-262202415355732.jpg)
![Western Blot: TRAF-2 Antibody (9A5) [NBP3-26271] - Positive Western Blot detected in: HepG2 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, K562 whole cell lysate. All lanes: TRAF-2 Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56, 62, 55, 54 kDa Observed band size: 56 kDa](http://images.novusbio.com/fullsize/nbp3-26271_rabbit-traf-2-mab-9a5-28220241140929.jpg)
![Flow Cytometry: TRAF-2 Antibody (9A5) [NBP3-26271] - Overlay histogram showing Hela cells stained with TRAF-2 Antibody (9A5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26271_rabbit-traf-2-mab-9a5-282202414183692.jpg)