TRAF-2 Antibody

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Western Blot: TRAF-2 Antibody - Unpurified [NB100-56173] - Analysis of TRAF-2 in normal human tissues using this antibody. TRAF-2 is observed at ~60-64 kDa. Additional bands of lower molecular weight were seen in some ...read more
Immunohistochemistry-Paraffin: TRAF-2 Antibody - Unpurified [NB100-56173] - Formalin-fixed, paraffin-embedded human colon carcinoma tissue sections stained for TRAF2 using this antibody at 1:2000. Hematoxylin-eosin ...read more
Western Blot: TRAF-2 Antibody - Unpurified [NB100-56173] - Detection of TRAF-2 protein in various tumor cell lines using this antibody at 1:2000 dilution.
Immunohistochemistry: TRAF-2 Antibody - Unpurified [NB100-56173] - Formalin-fixed, paraffin-embedded human melanoma tissue section stained for TRAF2 at 1:2000. A1 is a higher magnification of A. Hematoxylin-eosin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TRAF-2 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 6-31 (VTPPGSLELLQPGFSKTLLGTKLEAK) of human TRAF2 was used as immunogen, GenBank no. NP_066961.2. This peptide sequence is 92% conserved in mouse and rat TRAF2. The peptide immunogen sequence is 100% conserved in multiple human TRAF2 GenBank accession numbers. These TRAF2 accession numbers are of varying amino acid (aa) lengths and include: 1) NP_066961.2 = 501 aa. 2) CAI12705.1 =121 aa. 3) CAI12704.1 = 422 aa. 4) CAI15104.1 = 193 aa. 5) BAD96681.1 = 205 aa.
Specificity
The gene encoding TRAF2 produces, by alternative splicing, 15 different transcripts, all with introns, putatively encoding 15 different protein isoforms. These isoforms are of varying amino acid lengths. Therefore the molecular weight observed by western blot may vary, depending on the isoform expressed. Users are encouraged to use the peptide immunogen sequence information provided in the antigen section to gain additional information about the potential TRAF2 isoforms detected by the antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRAF2
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Control
TRAF-2 Overexpression Lysate
TRAF-2 Knockout 293T Cell Lysate
Publications
Read Publications using
NB100-56173 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for TRAF-2 Antibody

  • E3 ubiquitin-protein ligase TRAF2
  • EC 6.3.2.-
  • MGC:45012
  • TNF receptor-associated factor 2
  • TRAF2
  • TRAF-2
  • TRAP3
  • TRAP3TRAP
  • tumor necrosis factor type 2 receptor associated protein 3
  • Tumor necrosis factor type 2 receptor-associated protein 3

Background

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor super families and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. TRAF2 is typically described as a 501 amino acid protein, GenBank no. NP_066961.2. However, various TRAF2 isoforms have been described. Therefore, the molecular weight of TRAF2 detected by Western blot may vary depending on isoforms expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRAF-2 Antibody (NB100-56173)(3)

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRAF2
Entrez