TRAF-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: TRAF-2 Antibody [NB100-56172] - Analysis of TRAF-2 in formalin-fixed, paraffin-embedded normal human thymus sections using NB100-56172 at 1:2000. A-C, successively higher magnifications. Hematoxylin-eosin counterstain.

Immunohistochemistry: TRAF-2 Antibody [NB100-56172] - Analysis of TRAF2 in formalin-fixed, paraffin-embedded normal human bone marrow section using NB100-56172 at 1:2000. Hematoxylin-eosin counterstain.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRAF-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRAF-2 Antibody - BSA Free (NB100-56172) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-TRAF-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to amino acids 87-106 (SSSAFPDNAARREVESLPAV) of human TRAF2 was used as immunogen, GenBank no. NP_066961.2. This peptide sequence is 100% conserved in mouse and rat TRAF2. The peptide immunogen sequence is 100% conserved in multiple human TRAF2 GenBank accession numbers. These TRAF2 accession numbers are of varying amino acid (aa) lengths and include: 1. NP_066961.2 = 501 aa. 2. CAI12705.1 =121 aa. 3. CAI12704.1 = 422 aa. 4. CAI15104.1 = 193 aa. 5. BAD96681.1 = 205 aa.
• Specificity: The gene encoding TRAF2 produces, by alternative splicing, 15 different transcripts, all with introns, putatively encoding 15 different protein isoforms. These isoforms are of varying amino acid lengths. Therefore the molecular weight observed by western blot may vary, depending on the isoform expressed. Users are encouraged to use the peptide immunogen sequence information provided in the antigen section to gain additional information about the potential TRAF2 isoforms detected by the antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAF2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 33471802)
  • Immunohistochemistry
  • Immunohistochemistry-Frozen Use at an assay dependent dilution
  • Immunohistochemistry-Paraffin 1/1000 - 1/500
  • Immunoprecipitation
  • Western Blot 1/1000 - 1/200
• Control:

  TRAF-2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for TRAF-2 Antibody - BSA Free

• E3 ubiquitin-protein ligase TRAF2
• EC 6.3.2.-
• MGC:45012
• TNF receptor-associated factor 2
• TRAF2
• TRAF-2
• TRAP3
• TRAP3TRAP
• tumor necrosis factor type 2 receptor associated protein 3
• Tumor necrosis factor type 2 receptor-associated protein 3

Background

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. NB100-56172 recognizes TRAF2. TRAF2 is typically described as a 501 amino acid (aa) protein, GenBank no. NP_066961.2. However, various TRAF2 isoforms have been described. Therefore the molecular weight of TRAF2 detected by Western blot may vary depending on isoforms expressed, please see the Application Notes for additional details. IMG-5760 detects TRAF2 isoforms containing the peptide immunogen sequence listed in the Antigen section.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-2 Antibody (NB100-56172)(1)

Publication using NB100-56172

• Stanfield BA, Purves T, Palmer S, et al. IL-10 and class 1 histone deacetylases act synergistically and independently on the secretion of proinflammatory mediators in alveolar macrophages PloS one 2021-01-20 [PMID: 33471802] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: TRAF2
• Entrez:
  • 7186(Human)
  • 22030(Mouse)
  • 311786(Rat)