Tollip Antibody (503315)

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat Tollip Monoclonal Antibody (Catalog # MAB4678) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tollip at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Rt
• Applications: WB
• Clone: 503315
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Tollip Antibody (503315) Summary

• Immunogen: E. coli-derived recombinant human Tollip
  Met1-Pro274
  Accession # Q9H0E2
• Specificity: Detects human and rat Tollip in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOLLIP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tollip Antibody (503315)

• adapter protein
• FLJ33531
• IL-1RAcPIP
• toll interacting protein
• toll-interacting protein
• Tollip

Background

Tollip (Toll-interacting protein) is a 28 kDa, cytoplasmic protein that is involved in IL-1 and Toll receptor signaling. It is classified as an adaptor protein that performs multiple functions. It binds both IRAK and Toll receptors, inhibiting constitutive IL-1 and Toll receptor signaling. It also binds to ubiquinated IL-1 RI, directing it to the endosomal degradation compartment. Human Tollip is 274 amino acids(aa) in length. It contains a C2 Ca⁺⁺-binding region (aa 54‑154) and a CUE domain (aa 229‑270) that binds ubiquitin. One potential splice form exists that shows a deletion of aa 6‑33. Human Tollip is 92% identical to mouse Tollip.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tollip Antibody (MAB4678)(2)

Publications using MAB4678

• L Woli?ska-N, K Romaniuk, K Wojciechow, K Surga, M Kamaszewsk, H Szudrowicz, M Mi?czy?ska Tollip-deficient zebrafish display no abnormalities in development, organ morphology or gene expression in response to lipopolysaccharide FEBS Open Bio, 2022-06-27;0(0):. 2022-06-27 [PMID: 35506194] (Western Blot, Zebrafish)
• Torun A, Szymanska E, Castanon I, Wolinska-Niziol L, Bartosik A, Jastrzebski K, Mietkowska M, Gonzalez-Gaitan M, Miaczynska M Endocytic Adaptor Protein Tollip Inhibits Canonical Wnt Signaling. PLoS ONE, 2015-06-25;10(6):e0130818. 2015-06-25 [PMID: 26110841] (IHC, Zebrafish)

Bioinformatics

• Gene Symbol: TOLLIP
• Uniprot:
  • Q9H0E2(Human)