TOM1 Antibody (5A3) - Azide and BSA Free

Western Blot: TOM1 Antibody (5A3) [H00010043-M01] - TOM1 monoclonal antibody (M01), clone 5A3 Analysis of TOM1 expression in K-562.

Immunohistochemistry-Paraffin: TOM1 Antibody (5A3) [H00010043-M01] - Analysis of monoclonal antibody to TOM1 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 3 ug/ml.

Western Blot: TOM1 Antibody (5A3) [H00010043-M01] - Analysis of TOM1 expression in transfected 293T cell line by TOM1 monoclonal antibody (M01), clone 5A3.Lane 1: TOM1 transfected lysate(53.8 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: TOM1 Antibody (5A3) [H00010043-M01] - Detection limit for recombinant GST tagged TOM1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 5A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TOM1 Antibody (5A3) - Azide and BSA Free Summary

• Immunogen: TOM1 (NP_005479, 394 a.a. ~ 491 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GLAGALDARQQSTGAIPVTQACLMEDIEQWLSTDVGNDAEEPKGVTSEEFDKFLEERAKAADRLPNLSSPSAEGPPGPPSGPAPRKKTQEKDDDMLFA
• Specificity: TOM1 (5A3)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOM1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TOM1 Antibody (5A3) - Azide and BSA Free

• ADP-ribosylation factor-binding protein GGA3
• golgi associated, gamma adaptin ear containing, ARF binding protein 3
• golgi-associated, gamma adaptin ear containing, ARF binding protein 3
• KIAA0154Golgi-localized, gamma ear-containing, ARF-binding protein 3

Background

The specific function of this gene has not yet been determined, yet it may involve the translocation of growth factor receptor complexes to the lysosome for degradation. This gene is localized to 22q13.1, with HMOX1 and MCM5 distally and HMG2L1 proximally positioned.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOM1 Antibody (H00010043-M01)(1)

Publication using H00010043-M01

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: TOM1
• Entrez:
  • 10043(Human)
• OMIM:
  • 604700(Human)
• Uniprot:
  • NP_005479(Human)