IRAK4 Antibody (2H9)

Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.

Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human kidney cancer tissues using IRAK4 mouse mAb with DAB staining.

Flow Cytometry: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of Hela cells using IRAK4 mouse mAb (blue) and negative control (red).

Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mAb against human IRAK4 (AA: 21-198) recombinant protein. (Expected MW is 45.4 kDa)

Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human lung cancer tissues using IRAK4 mouse mAb with DAB staining.

ELISA: IRAK4 Antibody (2H9) [NBP2-37575] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu, Pm
• Applications: WB, ELISA, Flow, IHC, IP
• Clone: 2H9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IRAK4 Antibody (2H9) Summary

• Immunogen: Purified recombinant fragment of human IRAK4 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRAK4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500 - 1:2000
• Application Notes: This IRAK4 Antibody (2H9) is validated for IP from a verified customer review.
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for IRAK4 Antibody (2H9)

• EC 2.7.11
• EC 2.7.11.1
• interleukin-1 receptor-associated kinase 4
• IPD1
• IRAK4
• IRAK-4
• NY-REN-64
• REN64
• Renal carcinoma antigen NY-REN-64

Background

Interleukin-1 receptor (see IL1R; MIM 147810)-associated kinases (e.g., IRAK1; MIM 300283) are important mediators in the signal transduction of Toll-like receptor (TLR, e.g., TLR4; MIM 603030) and IL1R family members, collectively referred to as TIRs. IRAK4 functions in this signal transduction pathway.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRAK4 Antibody (NBP2-37575)(1)

Publication using NBP2-37575

• Stanfield B, Soderblom E, Palmer S Et Al. Histone Deacetylase 3 Associates With Key Regulators of NF kappa B Signaling in Lung Epithelial Cells Under Stimulation With LPS. Research Square 2021-07-07 (IP, ICC/IF)

Review for IRAK4 Antibody (NBP2-37575) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Brent Stanfield IP Human 02/07/2020

Summary

• Application: Immunoprecipitation
• Sample Tested: A549 human alveolar adenocarcinoma cell line
• Species: Human
• Lot: 190110

Comments

• Comments: 500 ug of whole cell lysates from cells either left untreated Left or treated with 10 uM MS275 selective Class 1 HDAC inhibitor, Right were immunoprecipitated using 10 uL of IRAK4 Antibody (2H9) conjugated to 50 uL of protein G Dynabeads. Antibody was crosslinked to beads to reduce background using BS3 reagent. following precipitation/washing bound proteins were eluted and ran on a 4-15% gel. Total acetylation was visualized using rabbit anti-acetylated-lysine. bands pictured visualized at ~55 kDA.

Bioinformatics

• Gene Symbol: IRAK4