TNF RI/TNFRSF1A Antibody - BSA Free

Western Blot: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF-R1 Antibody [NBP1-97453] - Analysis of TNF-R1: Lane 1: MW marker, Lane 2: Hela (HS), Lane 3: Jurkat.

Immunohistochemistry-Paraffin: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF-R1 Antibody [NBP1-97453] - Analysis of human spleen tissue stained with TNF receptor 1 pAb at 10ug/ml.

Flow Cytometry: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF Receptor I Antibody [NBP1-97453] - MLL-AF9 transduced murine leukemia cells. Tnfr-/- cells were used as negative control. Image provided by verified customer.

Western Blot: Rabbit Polyclonal TNF RI/TNFRSF1A Antibody [NBP1-97453] -Quantification of TNF alpha in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. NBP1-97453 was used at a dilution of 1:1000 and Beta-actin antibody (Catalog # NB600-501) was used at a dilution of 1:3000. Evaluation of pre-cellularized graft in iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to tibial bone defect, G2: cellularized graft for 6 weeks applied to tibial bone defect, G3: cellularized graft for 8 weeks applied to tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 10 days after graft implantation. Image from a verified customer review.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Dr, Gt, Ha, Pm, Rb, Sh, Xp, Ye
• Applications: WB, Flow, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TNF RI/TNFRSF1A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TNF RI/TNFRSF1A Antibody - BSA Free (NBP1-97453) is a polyclonal antibody validated for use in IHC, WB, Flow and IP. Anti-TNF RI/TNFRSF1A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to a portion of mouse TNF-R1. The sequence is completely conserved in rat and human.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNFRSF1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 12.5 ug/ml
  • Western Blot 1:1000
• Application Notes: TNF RI/TNFRSF1A antibody validated for FLOW from a verified customer review.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Yeast TNF-R1. Mouse and Goat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TNF RI/TNFRSF1A Antibody - BSA Free

• CD120a antigen
• CD120a
• FPF
• p55
• p55-R
• p60
• TNF RI
• TNFARMGC19588
• TNF-R
• TNF-R1
• TNFR1TBP1
• TNFR55
• TNF-R55
• TNFR60
• TNFRI
• TNF-RI
• TNF-R-I
• TNFR-I
• TNFRSF1A
• tumor necrosis factor binding protein 1
• Tumor necrosis factor receptor 1
• tumor necrosis factor receptor 1A isoform beta
• tumor necrosis factor receptor superfamily member 1A
• tumor necrosis factor receptor superfamily, member 1A
• tumor necrosis factor receptor type 1
• Tumor necrosis factor receptor type I
• tumor necrosis factor-alpha receptor

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF RI/TNFRSF1A Antibody (NBP1-97453)(3)

Publications using NBP1-97453

• James M. Murphy, Kyuho Jeong, Donna L. Cioffi, Pamela Moore Campbell, Hanjoong Jo, Eun-Young Erin Ahn, Ssang-Taek Steve Lim Focal Adhesion Kinase Activity and Localization is Critical for TNF-alpha -Induced Nuclear Factor-kappa B Activation Inflammation 2021-08-01 [PMID: 33527321]
• Pasupulati A, Nishad R, Mukhi D et al. Growth hormone induces TNF-alpha in podocytes and contributes to monocyte-to-macrophage differentiation: Implications in Diabetic kidney disease Research Square 2022-02-02
• H Yamawaki et al. Chronic physiological shear stress inhibits tumor necrosis factor-induced proinflammatory responses in rabbit aorta perfused ex vivo. Circulation 108, 1619. 2003-01-01 [PMID: 12963644] (IP, WB, Rabbit)

Reviews for TNF RI/TNFRSF1A Antibody (NBP1-97453) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Goat.

reviewed by: Verified Customer WB Goat 05/13/2025

Summary

• Application: Western Blot
• Sample Tested: Bone Extracts,Bone
• Species: Goat
• Lot: P4MA030

Comments

• Comments: ***Bio-Techne Response: This review reflects a new species or application tested on a primary antibody.***
  Evaluation of pre-cellularized graft in iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to tibial bone defect, G2: cellularized graft for 6 weeks applied to tibial bone defect, G3: cellularized graft for 8 weeks applied to tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 10 days after graft implantation.

reviewed by: Andrew Volk Flow Mouse 01/31/2014

Summary

• Application: Flow Cytometry
• Sample Tested: Mouse MLL-AF9 transduced leukemia cells
• Species: Mouse
• Lot: 03051232

Bioinformatics

• Gene Symbol: TNFRSF1A
• Entrez:
  • 7132(Human)