210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225). The ND50 is typically 0.008-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Human TNF RI/TNFRSF1A (Catalog # 372-R1) inhibits Recombinant Human TNF-a (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225). The ND50 is typically 0.008-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />
HAF109). A specific band was detected for TNF RI/TNFRSF1A at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of L-929 mouse fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RI/TNFRSF1A at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-425-SP | |||
636-R1) inhibits Recombinant Human TNF-a (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB625). The ND50 is typically 1-6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1) inhibits Recombinant Human TNF-a (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB625). The ND50 is typically 1-6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB625-SP | |||
| Carrier Free | MAB625-500 | |||
Fluorescent ICC Staining of Non-adherent Cells." alt="TNF RI/TNFRSF1A was detected in immersion fixed human peripheral blood lymphocytes using 5 µg/mL Human TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF225) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF225-SP | |||
410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB430). The ND50 is typically 5-20 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Mouse TNF-a (Catalog # 410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB430). The ND50 is typically 5-20 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB430-SP | |||
| Carrier Free | MAB430-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97453-0.2mg | |||
| BSA Free | NBP1-97453-0.025mg | |||
HAF005). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of C6 rat glioma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB425-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78785SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88140-25ul | |||
HAF018). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB6251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6251-SP | |||
MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ![Representative immunohistochemical staining for TNFR1 and TNFR2 in the kidneys.(A) Images were captured at 200× (a, b, c, d, i, j, k, l) and 100× (e, f, g, h, m, n, o, p) magnification. Images show the negative controls in the glomeruli (a, i) and tubulointerstitium (e, m) and TNFR1 and TNFR2 immunostaining in the glomeruli (b, j) and tubulointerstitium (f, n) of the normal kidneys, respectively. TNFR1 and TNFR2 immunostaining is shown in the glomeruli (c, k) and tubulointerstitium (g, o) of the kidneys from selected IgAN patients who had levels of serum TNFR2 that ranked in the lowest 10 [low group (LG)]. TNFR1 and TNFR2 immunostaining in the glomeruli (d, l) and tubulointerstitium (h, p) of the kidneys from selected IgAN patients who had levels of serum TNFR2 that ranked in the highest 10 [high group (HG)], respectively, are also shown. (B) Percentage of the TNFR1 and TNFR2-positive area in the kidneys were evaluated. Glomerular and tubulointerstitial TNFR expression was elevated significantly in IgAN patients compared with those in control (Ctrl) subjects. The tubulointerstitial TNFR2-positive area was significantly larger in HG than in LG. However, there was no significant difference in the tubulointerstitial TNFR1 and glomerular TNFR areas between LG and HG. * P < 0.01, **P < 0.001, †P < 0.0001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122212), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab225_human-tnf-ri-tnfrsf1a-mab-clone-16803-41202410481119.jpg)
AB-108-C, open histo-gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # 
425-R1) was serially diluted and captured by Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # 
636-R1) was serially diluted and captured by Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB625) coated on a Clear Polystyrene Microplate (Catalog # 
636-R1) was serially diluted and captured by Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # ![Western Blot: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF-R1 Antibody [NBP1-97453] - Analysis of TNF-R1: Lane 1: MW marker, Lane 2: Hela (HS), Lane 3: Jurkat.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Western-Blot-NBP1-97453-img0005.jpg "Western Blot: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF-R1 Antibody [NBP1-97453] - Analysis of TNF-R1: Lane 1: MW marker, Lane 2: Hela (HS), Lane 3: Jurkat.")
![Immunohistochemistry-Paraffin: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF-R1 Antibody [NBP1-97453] - Analysis of human spleen tissue stained with TNF receptor 1 pAb at 10ug/ml.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Immunohistochemistry-Paraffin-NBP1-97453-img0004.jpg)
![Flow Cytometry: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF Receptor I Antibody [NBP1-97453] - MLL-AF9 transduced murine leukemia cells. Tnfr-/- cells were used as negative control. Image provided by verified customer.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Flow-Cytometry-NBP1-97453-img0006.jpg)
![Western Blot: Rabbit Polyclonal TNF RI/TNFRSF1A Antibody [NBP1-97453] -Quantification of TNF alpha in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. NBP1-97453 was used at a dilution of 1:1000 and Beta-actin antibody (Catalog # NB600-501) was used at a dilution of 1:3000. Evaluation of pre-cellularized graft in iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to tibial bone defect, G2: cellularized graft for 6 weeks applied to tibial bone defect, G3: cellularized graft for 8 weeks applied to tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 10 days after graft implantation. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97453_rabbit-polyclonal-tnf-ri-tnfrsf1a-antibody-western-blot-2952025141129.jpg)
425-R1) was serially diluted and captured by Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) coated on a Clear Polystyrene Microplate (Catalog # ![Western Blot: TNF RI/TNFRSF1A Antibody [NBP2-78785] - Total protein from human and mouse liver and placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNFR1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Western-Blot-NBP2-78785-img0002.jpg "Western Blot: TNF RI/TNFRSF1A Antibody [NBP2-78785] - Total protein from human and mouse liver and placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNFR1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: TNF RI/TNFRSF1A Antibody [NBP2-78785] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of TNFRI antibody (NBP2-78785). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly moderately strong reactivity in glandular cell cytoplasm.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Immunohistochemistry-Paraffin-NBP2-78785-img0001.jpg)
![Flow Cytometry: TNF RI/TNFRSF1A Antibody (H398) - Azide Free [NBP2-37724] - Analysis (surface staining) of CD120a in human peripheral blood with anti-human CD120a (H398) purified, GAM-APC.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-H398-Azide-Free-Flow-Cytometry-NBP2-37724-img0001.jpg "Flow Cytometry: TNF RI/TNFRSF1A Antibody (H398) - Azide Free [NBP2-37724] - Analysis (surface staining) of CD120a in human peripheral blood with anti-human CD120a (H398) purified, GAM-APC.")
