TNF-alpha Antibody - Azide Free


Immunocytochemistry/ Immunofluorescence: TNF-alpha Antibody - Azide Free [NB600-587] - LMP2 knockdown by shRNA reduced the expression of TNF-alpha and IL-1beta after MCAO. Immunofluorescence labeling revealed that both more
Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Analysis using Azide/BSA FREE version of NB600-587. Membrane blocked in 1% BSA-TBS-T 30 min RT, 1:1000 dilution in 1% BSA-TBS-T 4C.
Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Expression of TNF-alpha, IL-1beta and NF-kappaB in the brains of MCAO rats. Western blot confirmed that protein levels of TNF-alpha increased as early as 4 h more
Immunohistochemistry: TNF-alpha Antibody - Azide Free [NB600-587] - Staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat more
Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Expression of TNF-alpha, IL-1beta and NF-kappaB in the brains of MCAO rats. Western blot confirmed that protein levels of TNF-alpha increased as early as 4 h more

Product Details

Reactivity Hu, Mu, Rt, Ch, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Azide Free

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TNF-alpha Antibody - Azide Free Summary

This antiserum has been heated to 56 C for 30 minutes.
The whole rabbit serum was prepared by repeated immunizations with recombinant human TNF alpha produced in E.coli.
Type 2 membrane protein or soluble form derived from the membrane by proteolytic cleavage.
The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNG beta (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500 - 1:2000
  • ELISA 1:1000 - 1:5000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Reviewed Applications
Read 1 Review rated 5
NB600-587 in the following applications:

Read Publications using
NB600-587 in the following applications:

  • IHC
    2 publications
  • 3 publications
  • WB
    8 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25894537), Mouse reactivity reported in scientific literature (PMID:22567116). Chicken reactivity reported in scientific literature (PMID: 30298003).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No Preservative
Delipidation and Defibrination

Alternate Names for TNF-alpha Antibody - Azide Free

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha


Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready
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Applications: WB, Simple Western, IHC, ICC, KO
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Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for TNF-alpha Antibody (NB600-587)(18)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 3 applications: IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB600-587 Applications Species
Bhowmick S, D'Mello V, Caruso D, Abdul-Muneer PM Traumatic brain injury-induced downregulation of Nrf2 activates inflammatory response and apoptotic cell death J. Mol. Med. Nov 22 2019 [PMID: 31758217]
Lai A, Ho L, Evashwick-Rogler TW et al. Dietary polyphenols as a safe and novel intervention for modulating pain associated with intervertebral disc degeneration in an in-vivo rat model PLoS ONE 2019 Oct 02 [PMID: 31577822] (WB, Mouse) WB Mouse
Singh WR, Devi HS, Kumawat S, et al. Angiogenic and MMPs modulatory effects of icariin improved cutaneous wound healing in rats Eur. J. Pharmacol. 2019 Jun 17 [PMID: 31220437] (WB, Rat) WB Rat
Song C, Wu YS, Yang ZY et al. Astrocyte-Conditioned Medium Protects Prefrontal Cortical Neurons from Glutamate-Induced Cell Death by Inhibiting TNF-a Expression Neuroimmunomodulation Jan 30 2019 [PMID: 30699428] (WB, Rat) WB Rat
Fauzia E, Barbhuyan Tk, Shrivastava Ak et al. Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism. Front Pharmacol. Sep 21 2018 [PMID: 30298003] (Chicken) Chicken
Evashwick-Rogler TW, Lai A, Watanabe H et al. Inhibiting tumor necrosis factor-alpha at time of induced intervertebral disc injury limits long-term pain and degeneration in a rat model. JOR Spine 2018 Jun [PMID: 29963655] (Rat) Rat
Cesur MG, Gulle K, Sirin FB et al. Effects of curcumin and melatonin on bone formation in orthopedically expanded suture in rats: A biochemical, histological and immunohistochemical study Orthod Craniofac Res Jun 21 2018 [PMID: 29927045] (IHC-P, Rat) IHC-P Rat
Abdel-Zaher AO, Farghaly HSM, El-Refaiy AEM, Abd-Eldayem AM. Protective effect of the standardized leaf extract of Ginkgo biloba (EGb761) against hypertension-induced renal injury in rats Clin. Exp. Hypertens. 2018 Jan 19 [PMID: 29351002] (Rat) Rat
Al-Khalaf HH, Amir M, Al-Mohanna F et al. Obesity and p16(INK4A) down-regulation activate breast adipocytes and promote their pro-tumorigenicity. Mol. Cell. Biol. 2017 Jun 19 [PMID: 28630279] (Human) Human
Gunel C, Demirci B, Eryilmaz A et al. Inhibitory Effect of Pycnogenol() on Airway Inflammation in Ovalbumin-Induced Allergic Rhinitis. Balkan Med J. Nov 1 2016 [PMID: 27994914] (IHC, Rat) IHC Rat
Show All 18 Publications.

Review for TNF-alpha Antibody (NB600-587) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-587:
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Images Ratings Applications Species Date Details
Western Blot TNF-alpha NB600-587
reviewed by:
WB Rat 05/22/2015


ApplicationWestern Blot
Sample TestedNRK whole cell lysate


Blocking Details5% NFDM for 1 h

Primary Anitbody

Dilution RatioTNF-alpha Antibody [NB600-587] 1:1000, overnight, 4 degree

Secondary Antibody

Secondary DescriptionPeroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG Secondary Antibody
Secondary Manufacturer Cat#Jackson ImmunoReasearch #95093
Secondary Concentration1:20000


Detection NotesECL using the Femto ECL ( Pierce #34095) and image on the Chemdoc. Exposure time: 5s

Product General Protocols

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ICC/IF Video Protocol

FAQs for TNF-alpha Antibody (NB600-587). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Recent Reviews


Application: WB
Species: Rat


Gene Symbol TNF