TLR9 Antibody (1138D) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
1138D
Clonality
Monoclonal
Host
Rabbit
Conjugate
Alexa Fluor 647

TLR9 Antibody (1138D) [Alexa Fluor® 647] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse TLR9
Leu26-Asp818
Accession # AAK29625
Specificity
Detects mouse TLR-9 in direct ELISAs.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLR9 Antibody (1138D) [Alexa Fluor® 647]

  • CD289 antigen
  • CD289
  • TLR9
  • toll-like receptor 9

Background

TLR9 (Toll‑like receptor 9), designated CD289, is a member of the TLR family of innate immune receptors that is mainly expressed by colonic epithelium, CD123+ plasmacytoid predendritic cells (pDC), and splenic transitional B cells (1‑9). TLR9 responds to unmethylated DNA CpG motifs that occur mainly in bacteria and viruses (1, 2). Mouse TLR9 cDNA encodes a 1032 amino acid (aa) type I transmembrane glycoprotein with a 793 aa extracellular domain (ECD) that contains 26 leucine‑rich repeats (LRRs, aa 26‑818), and a 193 aa cytoplasmic domain with a TIR sequence that dimerizes with signaling adaptors such as MyD88 (1). The mouse TLR9 ECD shares 87% aa sequence identity with rat and 71‑74% with human, feline, canine, equine, porcine, bovine and ovine TLR9. Predicted splice forms vary at the N‑terminus by initiating either upstream or downstream of the standard site. The full-length 150 kDa form, which is ligand‑binding but nonsignaling, is found in the endoplasmic reticulum. It undergoes accessory protein-mediated translocation either to the cell membrane or to lysosomes (1‑3). TLR9 is cleaved to remove LRR1‑14, producing an 80 kDa signaling fragment within acidic endolysosomes where it encounters microbial CpG DNA rather than self-DNA (2, 10, 11). However, immune complexes of self‑DNA with lupus erythematosus anti‑DNA antibodies can induce TLR9 activation and IFN‑ alpha production in pDC (4). A soluble form also found in endosomes includes all 26 LRRs and negatively regulates active TLR9 (12). Activation of TLR9 contributes to splenocyte proliferation, pDC maturation, macrophage inflammatory cytokine production, Th1 inflammatory responses, NK cell activation and recruitment, B cell surface MHC class II up‑regulation and immunoglobulin production, and generation and maintenance of memory B cells (1, 5‑9).

  1. Hemmi, H. et al. (2000) Nature 408:740.
  2. Barton, G.M. et al. (2006) Nat. Immunol. 7:49.
  3. Latz, E. et al. (2004) Nat. Immunol. 5:190.
  4. Barrat, F.J. et al. (2005) J. Exp. Med. 202:1131.
  5. Krieg, A.M. et al. (1995) Nature 374:546.
  6. Aranburu, A. et al. (2010) J. Immunol. 185:7293.
  7. Guerrier, T. et al. (2012) J. Autoimmun. 39:173.
  8. Guillerey, C. et al. (2012) Blood 120:90.
  9. Cunningham-Rundles, C. et al. (2006) J. Immunol. 176:1978.
  10. Ewald, S.E. et al. (2008) Nature 456:658.
  11. Park, B. et al. (2008) Nat. Immunol. 9:1407.
  12. Chockalingam, A. et al. (2011) Eur. J. Immunol. 41:2176.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Pathways for TLR9 Antibody (FAB7960R)

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Blogs on TLR9.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR9: Tollgate to Immunity
Toll-like receptors (TLRs) play an essential role in the activation of innate immunity, and TLRs are expressed in a large number of immune cells as well as in epithelial cells. TLR9 recognizes synthetic oligodeoxynucleotides (ODN) containing unmethyla...  Read full blog post.

TLR9, Infectious Disease and Cancer
Toll-like receptor 9 (TLR9) is a protein encoded by TLR9 gene in humans. It is also known as cluster of differentiation 289 (CD289) and is a member of TLR family. Proteins from TLR family are transmembrane proteins that expressed in both antigen-resen...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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