Human TLR6 Stable Cell Line

Product Discontinued

Human TLR6 Stable Cell Line Summary

• Description: The TLR6 stable cell line can be used for TLR6 flow cytometric calibration and detection control. TLR6 expression has been validated by Western blotting (fig. 1) and flow cytometry (fig. 2).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: TLR6 stable cell line is a stably transfected cell line which expresses full-length human Toll-like receptor 6 (TLR6) with an N-terminal HA tag.
• Target Species: Human
• Specificity: TLR6
• Selection Agent: Blasticidin.
• Growth Properties: Adherent Morphology: Epithelial
• RCL Type: Stable Cell Line
• Host: HEK293
• Gene: TLR6

Applications/Dilutions

• Readout System:
  • NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) NBP2-25286
  • Cell Surface Staining Flow Cytometry Kit NBP2-26247

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the stable line.
- Wash hands after handling the stable line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human TLR6 Stable Cell Line

• CD286 antigen
• CD286
• TLR6
• toll-like receptor 6

Background

TLR6 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR6 functionally interacts with toll-like receptor 2 to mediate cellular response to bacterial lipoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLR6