TLR6 Antibody [Unconjugated] (AF1533)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price

TLR6 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TLR6
Leu43-Asp594
Accession # BAA78632
Specificity
Detects mouse TLR6 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) TLR1 is observed and less than 1% cross-reactivity with rmTLR2, recombinant human (rh) TLR3 and rhTLR4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TLR6
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLR6 Antibody [Unconjugated]

  • CD286 antigen
  • CD286
  • TLR6
  • toll-like receptor 6

Background

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1‑4). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular microbial nucleic acids). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. The mouse TLR6 cDNA encodes a 795 amino acid (aa) precursor that includes a 27 aa signal sequence, a 557 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain. The ECD contains 14 Leu-rich repeats, and the cytoplasmic region contains one TIR domain (5). Within the ECD, mouse TLR6 shares 59% aa sequence identity with mouse TLR1 and 20‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 71%, 72%, and 86% aa sequence identity with bovine, human, and rat TLR6, respectively. TLR6 is expressed on the cell surface of macrophages, monocytes, neutrophils, and dermal endothelial cells in ligand-independent association with TLR2 (6‑9). TLR2 also associates with TLR1, a functional complex with specificity for distinct but related microbial ligands (6‑8). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycan, and glycosylphosphatidylinositols (7‑14). The cytoplasmic TIR domain is necessary and sufficient to initiate signal transduction which leads to activation of NF kappa B (7, 15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on TLR6.

Exploring Various Studies on TLR6 Expression
The protein TLR6 is one member of the large Toll-like receptor (TLR) family, which governs the activation of the innate immunity system and pathogen recognition in cells. The TLR family is highly conserved from Drosophila to humans, and all the family...  Read full blog post.

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Bioinformatics

Gene Symbol TLR6
Entrez
Uniprot