TLR6 Antibody (418601)

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR6 Monoclonal Antibody (Catalog # MAB1533, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 418601
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TLR6 Antibody (418601) Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with mouse TLR6
  Phe39-Thr806
  Accession # BAA78632
• Specificity: Detects mouse TLR6. Stains mouse TLR6 transfectants and not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TLR6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLR6 Antibody (418601)

• CD286 antigen
• CD286
• TLR6
• toll-like receptor 6

Background

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1‑4). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular microbial nucleic acids). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. The mouse TLR6 cDNA encodes a 795 amino acid (aa) precursor that includes a 27 aa signal sequence, a 557 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain. The ECD contains 14 Leu-rich repeats, and the cytoplasmic region contains one TIR domain (5). Within the ECD, mouse TLR6 shares 59% aa sequence identity with mouse TLR1 and 20‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 71%, 72%, and 86% aa sequence identity with bovine, human, and rat TLR6, respectively. TLR6 is expressed on the cell surface of macrophages, monocytes, neutrophils, and dermal endothelial cells in ligand-independent association with TLR2 (6‑9). TLR2 also associates with TLR1, a functional complex with specificity for distinct but related microbial ligands (6‑8). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycan, and glycosylphosphatidylinositols (7‑14). The cytoplasmic TIR domain is necessary and sufficient to initiate signal transduction which leads to activation of NF kappa B (7, 15).

1. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
2. Roeder, A. et al. (2004) Med. Mycol. 42:485.
3. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
4. Wetzler, L.M. (2003) Vaccine 21:S55.
5. Takeuchi, O. et al. (1999) Gene 231:59.
6. Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
7. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
8. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
10. Bulut, Y. et al. (2001) J. Immunol. 167:987.
11. Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
12. Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
13. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
14. Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.
15. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR6 Antibody (MAB1533)(4)

Publications using MAB1533

• Yan, D;Li, X;Wang, H;Li, B;Wang, W;Liao, Y;Tang, BZ;Wang, D; NIR-II aggregation-induced emission nanoparticles camouflaged with preactivated macrophage membranes for phototheranostics of pulmonary tuberculosis Nature protocols 2025-04-10 [PMID: 40210746] (Western Blot, Mouse)
• Li, B;Wang, W;Zhao, L;Wu, Y;Li, X;Yan, D;Gao, Q;Yan, Y;Zhang, J;Feng, Y;Zheng, J;Shu, B;Wang, J;Wang, H;He, L;Zhang, Y;Pan, M;Wang, D;Tang, BZ;Liao, Y; Photothermal therapy of tuberculosis using targeting pre-activated macrophage membrane-coated nanoparticles Nature nanotechnology 2024-02-21 [PMID: 38383890] (Flow Cytometry, Mouse)
• Yohei Takeda, Masahiro Azuma, Ryoko Hatsugai, Yukari Fujimoto, Masahito Hashimoto, Koichi Fukase et al. The second and third amino acids of Pam2 lipopeptides are key for the proliferation of cytotoxic T cells Innate Immunity 7/1/2018 [PMID: 29848176]
• C Hellwing, A Schoeniger, C Roessler, A Leimert, J Schumann Lipid raft localization of TLR2 and its co-receptors is independent of membrane lipid composition PeerJ, 2018-01-05;6(0):e4212. 2018-01-05 [PMID: 29312832] (ICC, Mouse)

Review for TLR6 Antibody (MAB1533) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 02/10/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 22633994
• Species: Mouse

Bioinformatics

• Gene Symbol: TLR6
• Uniprot:
  • BAA78632()