Human TLR5 Stable Cell Line

Product Discontinued

Human TLR5 Stable Cell Line Summary

• Description: The TLR5 stable cell line can be used for TLR5 flow cytometric calibration and detection control as well as TLR5-dependent functional assays. TLR5 expression in this stable cell line has been validated by Western blotting (fig. 1) and flow cytometry (fig. 2). Functional activity of this stable cell line has been validated by the NF-kB/SEAPorter™ Assay Kit (NBP2-25286, fig. 3).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: TLR5 stable cell line is a stably transfected cell line which expresses full-length human Toll-like receptor 5 (TLR5) with an N-terminal HA tag.
• Target Species: Human
• Specificity: TLR5
• Selection Agent: Blasticidin
• Growth Properties: Adherent Morphology: Epithelial
• RCL Type: Stable Cell Line
• Host: HEK293
• Gene: TLR5

Applications/Dilutions

• Agonist:
  • Flagellin, TLR5 Ligand NBP2-25289
• Readout System:
  • NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) NBP2-25286
  • Cell Surface Staining Flow Cytometry Kit NBP2-26247

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the stable line.
- Wash hands after handling the stable line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human TLR5 Stable Cell Line

• MGC126430
• MGC126431
• SLEB1FLJ10052
• TIL-3
• TIL3Toll/interleukin-1 receptor-like protein 3
• TLR5
• toll-like receptor 5

Background

The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLR5